Capillary electrophoresis for the determination of albumin binding capacity and free bilirubin in jaundiced neonates

Impact of Protein Binding Capacity and Daily Dosage of a Drug on Total Serum Bilirubin Levels in Susceptible Infants

“…Currently, TSB is the biochemical standard used to evaluate and manage neonatal hyperbilirubinemia [ 2 ]. Bilirubin, which is predominantly derived from hemoglobin degradation, is mainly bound to albumin in the blood, inhibiting its ability to cross the BBB [ 31 , 32 ]. Among the total bilirubin, not more than 0.01% constitutes circulating as free bilirubin (an unbound form) [ 33 ], and an even ratio of cord blood bilirubin/cord blood albumin at birth has been reported to be a good indicator for the prediction of neonatal hyperbilirubinaemia [ 34 ].…”

70 years of bilirubin sensing: towards the point-of-care bilirubin monitoring in cirrhosis and hyperbilirubinemia

“…CE has also been coupled with frontal analysis (FA) to monitor the evolution of free bilirubin in infants, thus revealing the information about bilirubin–albumin binding affinity. 68,69 A CE-based microfluidic microsystem has also been reported for bedside monitoring. 70,71 Although CE is a low cost, quick, and reproducible technique, it requires expertise for handling.…”

Drug affinity to immobilized target bio-polymers by high-performance liquid chromatography and capillary electrophoresis

“…One of the major advantage in the use of CE-FA is the small consumption of the sample subjected to the analysis; a promising application for clinical use has been proposed for the determination of the free bilirubin in the jaundiced serum in the neonatal management of hyperbilirubinaemia [138,139].…”