Capillary electrophoresis enantioselective separation of vigabatrin enantiomers by precolumn derivatization with dehydroabietylisothiocyante and UV–vis detection

Zhao, Shulin; Zhang, Rongcan; Wang, Hengshan; Ling, Tang; Pan, Yinming

doi:10.1016/j.jchromb.2006.01.018

Cited by 23 publications

(10 citation statements)

References 11 publications

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“…Table 3 shows that only L-Glu and L-Asp were found in both S-1 and S-2. The type and their content are similar [29][30][31][32] …”

Section: Quantitation Of Aasmentioning

confidence: 99%

On‐column labeling technique and chiral ligand‐exchange CE with zinc(II)‐L‐arginine complex as a chiral selector for assay of dansylated D,L‐amino acids

Yang

2009

Electrophoresis

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A novel on-column labeling method of amino acid (AA) enantiomers by using dansyl chloride (Dns-Cl) has been explored combined with chiral ligand-exchange CE (CLE-CE) technique and UV detection. Efficient labeling was achieved by sequential injection of buffer, Dns-Cl, AA enantiomers, Dns-Cl and buffer at 0.2 psi for 10.0, 3.0, 24.0, 3.0, and 10.0 s, respectively. After injection, the sandwich sections were allowed to react at room temperature for 35.0 min. With this procedure, successful on-column labeling and CLE-CE separation of 17 pairs AA enantiomers have been achieved with a buffer of 100.0 mM boric acid, 5.0 mM ammonium acetate, 3.0 mM ZnSO4 and 6.0 mM L-Arg at pH 8.4, giving nine pairs fully enantioresolved with resolution in between 2.0 and 5.1. CLE-CE of some individual and mixed pairs was also demonstrated, much the same as using pre-column labeling. As validated by both artificially prepared solutions and serum samples, this new method was shown to be applicable to the quantitative analysis, with a linear range between 14.0 muM and 3.7 mM, correlation coefficient above 0.99 and recovery in between 90.4% and 111.7%. It was also demonstrated that the migration time-temperature based curve allows for temperature determination in CE by using this new method.

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“…Table 3 shows that only L-Glu and L-Asp were found in both S-1 and S-2. The type and their content are similar [29][30][31][32] …”

Section: Quantitation Of Aasmentioning

confidence: 99%

On‐column labeling technique and chiral ligand‐exchange CE with zinc(II)‐L‐arginine complex as a chiral selector for assay of dansylated D,L‐amino acids

Yang

2009

Electrophoresis

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“…In particular, vigabatrin enantiomers could be separated within 25 min, giving a calibration curve ranging from 0.3 to 6.0 mg/mL with a detection limit of 0.15 mg/mL. Quantitation of the compounds of interest was obtained in human plasma and no interferences were found from endogenous AAs [58].…”

Section: Chiral Derivatization Agentsmentioning

confidence: 99%

Recent advances in amino acid analysis by CE

et al. 2007

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This paper describes a number of articles that have been published on amino acid (AA) analysis using CE during the period from June 2005 to May 2007. This review article follows the format of the previous articles of Smith (Electrophoresis 1999, 20, 3078-3083), Prata et al.. (Electrophoresis 2001, 22, 4129-4138), and Poinsot et al.. (Electrophoresis 2003, 24, 4047-4062 and Electrophoresis 2006, 27, 176-194). Several new developments in AA analysis with CE are reported describing the use of laser-emitting diodes for LIF, MS, and chips. In addition, we describe articles concerning clinical studies and neuroclinical applications.

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“…[5][6][7] In the direct separation mode different types of chiral selectors, such as cyclodextrines, macrocyclic antibiotics, carbohydrates, chiral crown ethers, calixarenes, proteins and chiral metal complexes (Ligand-exchange) are most commonly used as chiral selectors. [8][9][10][11][12] The principle of ligand-exchange is based on the formation of diastereomeric ternary mixed metal com- plexes between the chiral selector ligand and the analyte and was first introduced by Davankov and Roghozin in the late 1960s-early 1970s for conventional columns.…”

Section: Introductionmentioning

confidence: 99%

Enantioseparation of Dansylated Amino Acids by Ligand-exchange Capillary Electrophoresis Using L-phenylalaninamide, L-lysine or L-threonine as Chiral Selector

Mohr¹,

Hägele²,

Schmid³

2011

Croat. Chem. Acta

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Abstract. In recent years enantioseparation of both active pharmaceutical ingredients and bio molecules such as amino acids became more and more necessary because in most cases the two stereo forms exhibit different pharmacological effects. This article deals with the chiral separation of dansylated amino acids by ligand-exchange capillary electrophoresis using L-phenylalaninamide, L-lysine and L-threonine as chiral selectors. Experiments with different central metal ions such as Cu(II), Co(II), Cd(II), Ni(II) and Zn(II) were carried out. Optimal conditions were found out by studying the effect of the pH and the selector molarity on the chiral resolution. Best separation was obtained for the Cu(II)/L-lysine complex, showing a chiral resolution up to 17 for Dns-DL-Met. (doi: 10.5562/cca1762)

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Capillary electrophoresis enantioselective separation of vigabatrin enantiomers by precolumn derivatization with dehydroabietylisothiocyante and UV–vis detection

Cited by 23 publications

References 11 publications

On‐column labeling technique and chiral ligand‐exchange CE with zinc(II)‐L‐arginine complex as a chiral selector for assay of dansylated D,L‐amino acids

On‐column labeling technique and chiral ligand‐exchange CE with zinc(II)‐L‐arginine complex as a chiral selector for assay of dansylated D,L‐amino acids

Recent advances in amino acid analysis by CE

Enantioseparation of Dansylated Amino Acids by Ligand-exchange Capillary Electrophoresis Using L-phenylalaninamide, L-lysine or L-threonine as Chiral Selector

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