Capillary electrophoresis-based methods for the determination of lipids—A review

Otieno, Anthony C.; Mwongela, Simon M.

doi:10.1016/j.aca.2008.06.026

Cited by 41 publications

(32 citation statements)

References 113 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to the importance of these fatty acids in human diet and the possibility of economic fraud, this work has as the main objective to apply CE as an auxiliary analytical tool able to distinguish between enriched and natural eggs using the ω fatty acid profile as a chemical marker. [13][14][15][16][17][18][19][20][21][22][23] Such fraud can be serious, since no visual difference is found between enriched and natural chicken eggs and, according to a price survey in the local market, a dozen enriched eggs can cost up to 2.0 times the price paid for a dozen natural eggs.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Omega 3 Fatty Acid in Natural and Enriched Chicken Eggs by Capillary Zone Electrophoresis

2011

View full text Add to dashboard Cite

Qualitative differentiation between natural and enriched chicken eggs through omega (ω) 3 fatty acid profiles by capillary zone electrophoresis (CZE) under direct UV detection at 200 nm is proposed. The electrolyte background consisted of 12.0 mmol L -1 tetraborate buffer (pH 9.2) mixed with 12.0 mmol L -1 Brij 35, 17% acetonitrile (ACN) and 33% methanol (MeOH). Omega 3 fatty acid profile in chicken egg samples were analyzed by CZE system and confirmed by single-quadrupole mass spectrometry with an electrospray ionization probe set to negative ionization mode after sample preparation by the Folch method. The results showed that ω fatty acid profiles analyzed by the CZE approach can be used to chemical markers to monitor fraud, presenting simplicity, short analysis time (10 min) and low cost as advantages.

show abstract

Section: Introductionmentioning

confidence: 99%

Analysis of Omega 3 Fatty Acid in Natural and Enriched Chicken Eggs by Capillary Zone Electrophoresis

2011

View full text Add to dashboard Cite

show abstract

“…A variety of cellular metabolites have been assayed using single-cell CZE, including thiol-containing compounds [16], small molecules such as glucose [17], dopamine [18], and amino acids [19], as well as measures of enzyme and peptidase activity [2023]. Single-cell CZE has also been used to study different classes of lipid metabolism in cytosolic lysates and single cells [24], such as sphingolipids [25,26], glycosphingolipids [27], and phosphatidylinositides [28]. …”

Section: Introductionmentioning

confidence: 99%

Chemical fixation to arrest phospholipid signaling for chemical cytometry

Proctor

Sims

Allbritton

2017

Journal of Chromatography A

View full text Add to dashboard Cite

Chemical cytometry is a powerful tool for measuring biological processes such as enzymatic signaling at the single cell level. Among these technologies, single-cell capillary zone electrophoresis (CZE) has emerged as a powerful tool to assay a wide range of cellular metabolites. However, analysis of dynamic processes within cells remains challenging as signaling pathways are rapidly altered in response to changes in the cellular environment, including cell manipulation and storage. To address these limitations, we describe a method for chemical fixation of cells to stop the cellular reactions to preserve the integrity of key signaling molecules or reporters within the cell and to enable the cell to act as a storage reservoir for the reporter and its metabolites prior to assay by single-cell CZE. Fluorescent phosphatidylinositol 4,5-bisphosphate reporters were loaded into cells and the cells were chemically fixed and stored prior to analysis. The reporter and its metabolites were electrophoretically separated by singlecell CZE. Chemical fixation parameters such as fixative, fixation time, storage solution, storage duration, and extraction solution were optimized. When cells were loaded with a fluorescent C6- or C16-PIP2 followed by glutaraldehyde fixation and immediate analysis, 24 ± 2% and 139 ± 12% of the lipid was recoverable, respectively, when compared to an unfixed control. Storage of the cells for 24 h yielded recoverable lipid of 61 ± 3% (C6-PIP2) and 55 ± 5% (C16-PIP2) when compared to cells analyzed immediately after fixation. The metabolites observed with and without fixation were identical. Measurement of phospholipase C activity in single leukemic cells in response to an agonist demonstrated the capability of chemical fixation coupled to singlecell CZE to yield an accurate snapshot of cellular reactions with the probe. This methodology enables cell assay with the reporter to be separated in space and time from reporter metabolite quantification while preserving assay integrity.

show abstract

“…In the analysis of PUFA, CE can be considered a viable alternative and also a complementary method to the well-established chromatographic techniques such as HPLC and GC. Although CE was used traditionally for the determination of high-molecular-mass biomolecules, its significance in the analysis of low-molecular-mass ionic species increased rapidly in the last years [4,5].…”

Section: Introductionmentioning

confidence: 99%

“…Capillary electrophoresis in the analysis of polyunsaturated fatty acids Several CE techniques can be used in the analysis of fatty acids such as capillary zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC) and microemulsion electrokinetic chromatography (MEEKC) employing different detection systems such as ultraviolet diode array detection (UV-DAD), laser induced fluorescence (LIF) or mass -spectrometry (MS) [4,5].…”

Section: Introductionmentioning

confidence: 99%

Capillary Electrophoresis in the Analysis of Polyunsaturated Fatty Acids

Hancu

Tero-Vescan

Filip

et al. 2015

Acta Medica Marisiensis

View full text Add to dashboard Cite

The aim of this study to inventory the main electrophoretic methods for identification and quantitative determination of fatty acids from different biological matrices. Critical analysis of electrophoretic methods reported in the literature show that the determination of polyunsaturated fatty acids can be made by: capillary zone electrophoresis, micellar electrokinetic chromatography and microemulsion electrokinetic chromatography using different detection systems such as ultraviolet diode array detection, laser induced fluorescence or mass -spectrometry. Capillary electrophoresis is a fast, low-cost technique used for polyunsaturated fatty acids analysis although their determination is mostly based on gas chromatography.Keywords: polyunsaturated fatty acids, capillary electrophoresis, omega 3 fatty acids Introduction Polyunsaturated fatty acids (PUFA) and especially the omega 3 fatty acids (FA) -eicosapentaenoic acid and docosahexaenoic acid and omega 6 FA -arachidonic acid present a special importance in physiological processes; fluidity of biological membranes is influenced by incorporating them into phospholipids structure.Due to the importance of FAs in different biochemistry and chemistry processes, elaboration of new methods of analysis for their determination represents a challenge and also a necessity for the analysts.The classical separation technique for FA analysis is the gas chromatography (GC)using flame ionization (FID) or mass spectrometry (MS) detectors. This FA methodology analysis involves firstly lipid fraction extraction, saponification reaction and then derivatization of the total FA content into fatty acid methyl esters (FAME) before injection in the GC equipment. Modern GC methods with high-quality capillary columns allow sensitive and reproducible FA analyses, as well as the characterization of complex mixtures of geometric isomers when combined with other chromatographic separations and spectroscopic identification. However GC analysis of FAshas its drawbacks related to the relatively long analysis times, the use of specialized expensive columns and to the necessity of precoloumn derivatization in order to improve volatility of the samples [1,2].Although GC is the predominant technique used for FAs analysis, high-performance liquid chromatography (HPLC) has also gained an important role in applications such as the handling of less usual samples, avoidance of degradation of heat-sensitive functional groups, and for micro-preparative purposes [3].

show abstract

Capillary electrophoresis-based methods for the determination of lipids—A review

Cited by 41 publications

References 113 publications

Analysis of Omega 3 Fatty Acid in Natural and Enriched Chicken Eggs by Capillary Zone Electrophoresis

Analysis of Omega 3 Fatty Acid in Natural and Enriched Chicken Eggs by Capillary Zone Electrophoresis

Chemical fixation to arrest phospholipid signaling for chemical cytometry

Capillary Electrophoresis in the Analysis of Polyunsaturated Fatty Acids

Contact Info

Product

Resources

About