Capillary electrophoresis as a sample separation step to mass spectrometry analysis: A primer

Lapizco‐Encinas, Blanca H.; Zhang, Y. Victoria; Gqamana, Putuma P.; Křenková, Jana; Foret, František

doi:10.1016/j.trac.2023.117093

Cited by 2 publications

(1 citation statement)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The recent developments in the area of MS detection in CE methods have been described in several reviews dealing with CE-MS in general [195][196][197][198] and with its application to peptide, protein, peptidomic, proteomic, and metabolomic analyses especially [8,16,44,[199][200][201].…”

Section: Mass Spectrometrymentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

View full text Add to dashboard Cite

This review article brings a comprehensive survey of developments and applications of high‐performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical characterization of peptides in the period from 2021 up to ca. the middle of 2023. Progress in the study of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, adsorption suppression, electroosmotic flow regulation, and detection, are presented. New developments in the particular capillary electromigration methods are demonstrated, and several types of their applications are reported. They cover qualitative and quantitative analysis of synthetic or isolated peptides and determination of peptides in complex biomatrices, peptide profiling of biofluids and tissues, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They include also amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical characterization of peptides and their interactions with other (bio)molecules by the above CE methods are described.

show abstract