Capillary electrophoresis as a metabolomics tool for non-targeted fingerprinting of biological samples

Barbas, Coral; Moraes, Edgar P.; Villaseñor, Alma

doi:10.1016/j.jpba.2011.02.001

Cited by 88 publications

(54 citation statements)

References 66 publications

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“…Furthermore, it is noteworthy that MS can be used to perform both qualitative and quantitative analyses, thus allowing classification of samples by means of metabolomic fingerprinting and subsequent identification of altered metabolites via fragmentation experiments. Mass spectrometry is usually coupled to various separation techniques, such as liquid chromatography (LC), gas chromatography (GC), or capillary electrophoresis (CE), in order to resolve isobaric interferences and to reduce ion suppression [3,4]. Nevertheless, these hyphenated approaches considerably increase the total analysis time and, consequently, the inter-sample variability, which hinders obtaining valid biological information from metabolomic data.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic Fingerprinting of Blood Samples by Direct Infusion Mass Spectrometry: Application in Alzheimer’s Disease Research

González‐Domínguez

2017

J. Anal. Test.

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Metabolomics is largely employed in numerous biomedical research fields, such as the study of the underlying pathology of diseases, discovery of diagnostic biomarkers, or drug development. Nowadays, the main challenge is to obtain comprehensive and unbiased metabolomic profiles due to the huge complexity, heterogeneity, and dynamism of the metabolome. To this end, mass spectrometry represents a very interesting analytical platform, since the complexity of metabolome may be overcome through the use of different orthogonal separation techniques, including liquid chromatography, gas chromatography, and capillary electrophoresis. Alternatively, direct mass spectrometry analysis has been postulated as a complementary choice to hyphenated approaches. This technique exhibits several advantages such as the ability for high-throughput screening, fast analysis, and wide metabolomic coverage, since there is not exclusion of compounds due to the separation device. The present work explores the utility of metabolomics based on direct infusion mass spectrometry for analyzing blood samples. The most important analytical concerns to be considered are discussed, including sample handling, comprehensive fingerprinting, as well as subsequent identification of metabolites, and global characterization of metabolomic profiles. To conclude, a brief review on the application of these metabolomic platforms in Alzheimer's disease research is also provided.

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Section: Introductionmentioning

confidence: 99%

Metabolomic Fingerprinting of Blood Samples by Direct Infusion Mass Spectrometry: Application in Alzheimer’s Disease Research

González‐Domínguez

2017

J. Anal. Test.

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“…(3) CE separates molecules based mainly on charge and size, and is particularly suitable for the separation of polar and charged metabolites. Combining CE with MS offers high resolution, with short analysis times (often in 10 min) and very small sample requirements (injection volumes ranging from 1 to 20 nL) [26] . Thus, CE-MS is a powerful and promising technique for high-throughput metabolomics of polar charged metabolites.…”

Section: Ms-based Methods For Metabolomics Ms Re-mentioning

confidence: 99%

Metabolomics: a novel approach to identify potential diagnostic biomarkers and pathogenesis in Alzheimer’s disease

Huang

Wang

et al. 2012

Neurosci. Bull.

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Although the pathogenesis of Alzheimer's disease (AD) is still not fully understood, it is acknowledged that intervention should be made at the early stage. Therefore, identifying biomarkers for the clinical diagnosis is critical.Metabolomics, a novel "omics", uses methods based on low-molecular-weight molecules, with high-throughput evaluation of a large number of metabolites that may lead to the identification of new disease-specific biomarkers and the elucidation of pathophysiological mechanisms. This review discusses metabolomics investigations of AD and potential future developments in this field.

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“…A CE-MS vem sendo usada como técnica complementar a LC-MS e GC-MS, [38][39][40] pois promove a separação de compostos iônicos polares. Possui alta resolução e curtos tempos de análise.…”

Section: Figura 1 Esquema Resumido Do Fluxograma De Trabalho Envolviunclassified

Metabolômica: Definições, Estado-Da-Arte E Aplicações Representativas

Canuto¹,

Costa²,

Cruz³

et al. 2017

Quim. Nova

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METABOLOMICS: DEFINITIONS, STATE-OF-THE-ART AND REPRESENTATIVE APPLICATIONS.Metabolomics is an emerging and promising omics approach used to understand biological mechanisms. By untargeted and targeted metabolomics analyses, metabolites are determined in biological samples (fluids, cells, tissues, etc.) by comparison of control groups with altered groups, undergoing different therapies, submitted to differing stress levels, dietary modulation, or promoted by a disease, or specific condition, etc., using sophisticated analytical techniques, and advanced data treatment and statistical analyses. In this review, the concepts involved in metabolomics studies were presented, describing in details all steps involved in the metabolomics workflow, for untargeted and targeted strategies. Finally, the potential of metabolomics is illustrated by applications in representative areas: clinical, environmental, food and nutrition, forensic toxicology, microbiology, parasitology, plants, and sports. Relevant reviews were compiled to characterize each of these areas, and a corresponding application of untargeted and targeted metabolomics were described.Keywords: metabolomics; untargeted metabolomics; targeted metabolomics; metabolomics workflow. INTRODUÇÃOAs ciências ômicas buscam o entendimento do funcionamento celular dos organismos e suas alterações biológicas. Fazem parte deste conjunto de ciências, a genômica (estudo da alteração dos genes), a transcriptômica (estudo das alterações dos transcritos), a proteômica (estudo das alterações das proteínas), e a metabolômica (estudo das alterações dos metabólitos). Metabólitos são produtos intermediários ou finais do metabolismo em uma amostra biológica.1 O conjunto de todos os metabólitos de baixa massa molecular (até 1500 Da), presentes ou alterados em um sistema biológico, é chamado de metaboloma (do inglês, metabolome).2 A pesquisa relacionada a metabólitos vem sendo desenvolvida há décadas, mas em 1999, Nicholson et al. definiram a metabonômica (do inglês, metabonomics), como sendo a medida quantitativa da resposta metabólica de um sistema biológico após estímulos fisiopatológicos ou modificações genéticas.3 Já o termo metabolômica (do inglês, metabolomics) foi introduzido em 2001, por Oliver Fiehn, como sendo a análise abrangente e quantitativa do metaboloma de um sistema biológico. 4 Além disso, a literatura nos apresenta outras denominações envolvendo esse campo da ciência, tais como: perfil metabólico (do inglês, metabolic profiling), descrito como sendo a análise de metabólitos previamente selecionados de rotas bioquímicas específicas, 5 a impressão digital metabólica (do inglês, metabolic fingerprinting), que é definida como sendo "uma classificação de amostras de acordo com sua origem ou sua relevância biológica", 3 e, por último, análise footprinting (ainda sem tradução para o português), para se referir aos metabólitos excretados por uma célula em condições controladas. Observa-se, portanto, uma divergência em relação às terminologias aplicadas às análises metabolômic...

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Capillary electrophoresis as a metabolomics tool for non-targeted fingerprinting of biological samples

Cited by 88 publications

References 66 publications

Metabolomic Fingerprinting of Blood Samples by Direct Infusion Mass Spectrometry: Application in Alzheimer’s Disease Research

Metabolomic Fingerprinting of Blood Samples by Direct Infusion Mass Spectrometry: Application in Alzheimer’s Disease Research

Metabolomics: a novel approach to identify potential diagnostic biomarkers and pathogenesis in Alzheimer’s disease

Metabolômica: Definições, Estado-Da-Arte E Aplicações Representativas

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