Capillary electrophoresis apparatus equipped with a bioluminescence detector using a batch- or flow-type detection cell

Tsukagoshi, Kazuhiko; Tahira, Masayuki; Nakajima, Riichiro

doi:10.1016/j.chroma.2005.09.048

Cited by 11 publications

(3 citation statements)

References 17 publications

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“…Fluorescence spectroscopy can therefore be used not only as a detector of analytes, but as a probe into the fundamental processes of electrophoretic separations. Other techniques that can be used to monitor electrophoretic separations are bioluminescence , chemiluminescence , phosphorescence , and electrogenerated chemiluminescence ; however these techniques will not be discussed in order to focus on tools to study interfaces and mass transport.…”

Section: Introductionmentioning

confidence: 99%

A fluorescence toolbox: A review of investigation of electrophoretic separations, process, and interfaces

Thompson

Pappas

2018

Electrophoresis

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This review focuses on fluorescence spectroscopy techniques for the investigation of electrophoretic separations. Fluorescence has been used as a sensitive detector for capillary, gel, and microchip electrophoresis for decades. However, advanced fluorescence methods can be used to study transport, interfacial phenomena, intermolecular and affinity interactions, and other processes that occur during separation. This so-called spectroscopic toolkit can be implemented to understand fundamental behavior in electrophoresis and electrokinetic chromatography. Techniques such as fluorescence recovery after photobleaching, fluorescence correlation spectroscopy, and fluorescence anisotropy are discussed in relation to electrophoretic separations. Newer methods such as super-resolution microscope are also introduced.

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Section: Introductionmentioning

confidence: 99%

A fluorescence toolbox: A review of investigation of electrophoretic separations, process, and interfaces

Thompson

Pappas

2018

Electrophoresis

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“…Later, Tsukagoshi et al [80] used a batch-type BL-detection cell to detect ATP using the firefly luciferase reaction, with an LOD of 1 μM ATP.…”

Section: Other Luminescence Approachesmentioning

confidence: 99%

Electrochemical and optical detectors for capillary and chip separations

Weber

2007

TrAC Trends in Analytical Chemistry

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In separations in capillaries or on chips, the most predominant detectors outside of the field of proteomics are electrochemical (EC) and optical. These detectors operate in the μM to pM range on nL peak volumes with ms time resolution. The driving forces for improvement are different for the two classes of detectors.With EC detectors, there are two limitations that the field is trying to overcome. One is the everpresent surface of the electrode which, while often advantageous for its catalytic or adsorptive properties, is also frequently responsible for changes in sensitivity over time. The other is the decoupling of the electrical systems that operate electrokinetic separations from the system operating the detector.With optical detectors, there are similarly a small number of important limitations. One is the need to bring the portability (size, weight and power requirements) of the detection system into the range of EC detectors. The other is broadening and simplifying the applications of fluorescence detection, as it almost always involves derivatization.Limitations aside, the ability to make detector electrodes and focused laser beams of the order of 1 μm in size, and the rapid time response of both detectors has vaulted capillary and chip separations to the forefront of small sample, fast, low mass-detection limit analysis.

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“…[6][7][8][9][10] Many types of reaction spaces have been proposed and investigated in CL analysis systems to achieve the above-mentioned CL advantages; however, they can be roughly classified into two types of reaction area, i.e., batch reaction and flow-mixing reaction types. [11][12][13][14][15] We have been studying a specific CL reaction area around the liquid-liquid interface in a micro-channel under laminar-flow conditions. [16][17][18][19] In this report, we call the analytical system taking advantage of the specific CL area micro-channel chemiluminescence analysis (MCCLA).…”

Section: Introductionmentioning

confidence: 99%

Micro-channel Chemiluminescence Analysis Using a Peroxyoxalate Reaction that Works through Liquid-Liquid Interface Collapse under Laminar-Flow Conditions

et al. 2008

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Capillary electrophoresis apparatus equipped with a bioluminescence detector using a batch- or flow-type detection cell

Cited by 11 publications

References 17 publications

A fluorescence toolbox: A review of investigation of electrophoretic separations, process, and interfaces

A fluorescence toolbox: A review of investigation of electrophoretic separations, process, and interfaces

Electrochemical and optical detectors for capillary and chip separations

Micro-channel Chemiluminescence Analysis Using a Peroxyoxalate Reaction that Works through Liquid-Liquid Interface Collapse under Laminar-Flow Conditions

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