Capillary electrophoresis and its application in the clinical laboratory

Petersen, John R.; Okorodudu, Anthony O.; Mohammad, Amin A.; Payne, Deborah

doi:10.1016/s0009-8981(03)00006-8

Cited by 108 publications

(88 citation statements)

References 197 publications

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“…In CZE, separation of protein fractions occurs in a free liquid medium created by the low viscosity buffer, in which the high voltage applied generates an electro-osmotic flow that forces the proteins to rapidly move toward the cathode (Giordano and Paltrinieri 2010). This allows better separation of proteins with similar physicochemical characteristics, thus generating multiple sub-peaks or narrower peaks (Petersen et al 2003). The higher resolution of CZE can often result in abnormal electrophoretic profiles caused by the aforementioned multiple sub-peaks of unknown significance, which is the disadvantage of this method.…”

Section: The Globulin Fractionsmentioning

confidence: 99%

Serum proteins and their diagnostic utility in veterinary medicine: a review

Tóthová¹,

Nagy²,

Kováč³

2016

Vet. Med.

130

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ABSTRACT:The evaluation of serum proteins and their electrophoretic pattern is a well-established laboratory method in the diagnosis of many diseases in humans, which has replaced the biochemical determination of the concentrations of albumin and the ratio of albumin to globulins. The measurement of serum proteins may be an important diagnostic tool for the detection, diagnosis, and monitoring of various diseases and pathological processes. The results of serum protein electrophoresis can be one of the most useful diagnostic aids in a wide spectrum of diseases, including infectious and inflammatory diseases, renal or gastrointestinal disorders, immunodeficiency states, as well as paraproteinaemias caused by lymphoid or plasma cell neoplasia. Although many studies have been carried out to determine the usefulness of the determination of serum proteins and their electrophoretic pattern in various disease conditions and disorders also in animals, serum protein evaluation is still a relatively little-used diagnostic tool in veterinary medicine. In this article, methods of serum protein determination, their diagnostic utility in animal care practice and their different patterns in dysproteinaemias and paraproteinaemias are reviewed.

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Section: The Globulin Fractionsmentioning

confidence: 99%

Serum proteins and their diagnostic utility in veterinary medicine: a review

Tóthová¹,

Nagy²,

Kováč³

2016

Vet. Med.

130

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“…[14]]. A great advantage of this over the conventional electrophoretic techniques is that, although also based on the movement of molecules in an electric field, CE is not restricted to the separation of large molecules based on size or charge.…”

Section: Capillary Electrophoresis: Potential and Use In Saliva Studymentioning

confidence: 99%

“…Besides Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS PAGE) and 2DE, salivary protein and peptide separation have been described to be achieved through capillary electrophoresis (CE). The possibility of work with small sample volumes and to perform high-resolution analysis is a great advantage of CE [14]. Although only few studies about salivary protein/peptide profiles related to ingestive behaviour have used CE, we believe that this area of research can gain from the use of this approach.…”

Section: Introductionmentioning

confidence: 99%

The Use of Electrophoresis for the Study of Saliva Involvement in Ingestive Behavior

Lamy¹,

Morzel²,

Rodrigues³

et al. 2015

Field Effect Electroosmosis - A Novel Phenomenon in Electrokinetics and Its Applications in Capillary Electrophoresis

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“…All CE modes employ the same instrumentation; samples can be easily analyzed subsequently as diff erencing separation mechanisms. Generally, CE is a very promising tool for clinical analysis at the present time [14][15][16][17][18][19] and it could be a simple and less time-consuming alternative technique to HPLC and GC which are also based on diff erent separation mechanisms than CE. The selectivity of CE can be utilized for separation of complicated mixtures of target analytes (e.g.…”

Section: Introductionmentioning

confidence: 99%

Capillary Electrophoresis as a Verification Tool for Immunochemical Drug Screening

Znaleziona¹,

Petr²,

Maier³

et al. 2007

BIOMED PAP

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Background:The aim of this work was to develop a simple capillary electrophoretic method as the verifi cation and confi rmation tool in the screening analysis for amphetamines, opiates, benzodiazepines and cocaine and their metabolites for toxicological applications.Methods: 50 mM phosphate Tris pH 2.0 with 30 % (v/v) of methanol was used as a background electrolyte that enabled fast separation of drugs and their metabolites in saliva and urine. Verifi cation of the data from the electrophoretic method was done by High Performance Thin Layer Chromatography (HPTLC) and the immunochemical screening test QuikScreen.Results: The experimental conditions of the Capillary Electrophoresis (CE) were partially optimized (mainly the infl uence of concentration and types of additives, e.g. cyclodextrines, organic solvents) and validated; the method was used for analysing samples from drug abusers.Conclusions: The non-instrumental, immunoassay tests could only confi rm qualitative addictions and are mainly employed when the emergency detection of drugs is needed. For quantitative analysis and verifi cation of obtained results the confi rmation step is strongly recommended. The simple screening capillary zone electrophoresis method allows recognition of the most abused drugs. The agreement of the results from CE, HPTLC and QuikScreen test was more than 95 %.

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Capillary electrophoresis and its application in the clinical laboratory

Cited by 108 publications

References 197 publications

Serum proteins and their diagnostic utility in veterinary medicine: a review

Serum proteins and their diagnostic utility in veterinary medicine: a review

The Use of Electrophoresis for the Study of Saliva Involvement in Ingestive Behavior

Capillary Electrophoresis as a Verification Tool for Immunochemical Drug Screening

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