Capillary electrophoresis and capillary electrophoresis ‐ electrospray ‐ mass spectroscopy for the analysis of heterocyclic amines

Zhao, Yining; Schelfaut, Marc; Sandra, Pat; Banks, Fred

doi:10.1002/elps.1150191228

Cited by 28 publications

(6 citation statements)

References 25 publications

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“…It has been reported that interfacing CE to MS may lead to peak broadening; however, it was observed here that the relative contribution to broadening varied with the amount of pressure used. The data in Tables and can be used to compare peak parameters between CE-UV and CE-MS detection for peptide KR for this purpose.…”

Section: Resultscontrasting

confidence: 57%

Advantages of Pressurization in Capillary Electrophoresis/Electrospray Ionization Mass Spectrometry

Hau

Roberts

1999

Anal. Chem.

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In this paper we present a simple interface for coupling capillary electrophoresis (CE) to mass spectrometry (MS) which can operate without significant user intervention. The use of a dedicated interface allows rapid changeover between CE/MS and liquid chromatography (LC)/MS, which is particularly important as CE and LC are often used on the same mass spectrometer. The suitability of the interface construction is illustrated on a triple quadrupole mass spectrometer and a high-resolution time-offlight mass spectrometer with compounds such as peptides, vitamins, and carbohydrates. Separations of these compounds are also used to evaluate the robustness of the CE-MS interface under pressurized and nonpressurized modes of operation. The simultaneous application of pressure and voltage overcomes two of the major problems commonly encountered when coupling CE to MS, i.e. unreliable long-term operation and protracted analysis times due to the long separation capillaries needed to connect to the mass spectrometer. Concomitant application of high voltage and hydrostatic pressure significantly reduces analysis time without compromising the overall separation. It was observed that pressurized CE exhibits less run-to-run-variations, leading to run-to-run repeatability better than 0.5% for migration time and below 5% for peak area with the MS as detector.

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Section: Resultscontrasting

confidence: 57%

Advantages of Pressurization in Capillary Electrophoresis/Electrospray Ionization Mass Spectrometry

Hau

Roberts

1999

Anal. Chem.

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“…At pH 2, the peaks were completely resolved, but the resolution gradually decreased towards pH 4, where the peaks were not resolved (data not shown). This is in agreement with results from other studies, which have shown that CE separation of HAs is best performed around pH 2-3 [17,19,29]. The decreasing resolution with increasing pH is a result of the pK a of the HAs.…”

Section: Ce-uv Experiments For Evaluation Of Electrolyte Ph For Separasupporting

confidence: 92%

“…Commonly, HAs are separated using HPLC [15,16]. HAs have also been analysed using CE-UV [17,18,19] and CE-MS [19].…”

Section: Introductionmentioning

confidence: 99%

In-capillary micro solid-phase extraction and capillary electrophoresis separation of heterocyclic aromatic amines with nanospray mass spectrometric detection

Viberg

Nilsson

Skog

2004

Analytical and Bioanalytical Chemistry

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A miniaturised technique to analyse and detect heterocyclic aromatic amines (HAs) using micro solid-phase extraction ( microSPE) coupled on-line (in-capillary) to capillary electrophoresis (CE) separation with nanospray (nESI) mass spectrometry (MS) detection has been developed. HAs are mutagenic and carcinogenic compounds formed at low levels in protein-rich food during cooking. Due to the low concentrations of HAs and the high complexity of the matrix in which they exist, sensitive and selective analytical methods are required for quantification. microSPE was performed on a packed bed of C(18 )particles inside the CE capillary, which minimised the dead volume. The on-line coupling of SPE, CE and nESI-MS reduced the time for extraction and identification to less than half an hour, which will allow for screening of several samples per day. The new technique provides short analysis time, low sample and solvent consumption, and HAs in standard solutions were easily detected at 12-17 fmol injections, and in spiked urine samples at 750-810 fmol injections.

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“…Only a few groups reported the use of ion trap instruments in the MS 32 or MS/MS mode 27, 28, 35. A small number of studies based on CE/MS have been described 36…”

Section: Introductionmentioning

confidence: 99%

Multistep mass spectrometry of heterocyclic amines in a quadrupole ion trap mass analyser

et al. 2002

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The fragmentation of heterocyclic amines (HAs) in an ion trap was studied by means of the infusion of methanolic solutions containing the compounds under assay, and using an atmospheric pressure chemical ionization (APCI) as ion source. The MS(n) spectra obtained for compounds included in the same family, either aminoimidazoazaarenes (AIAs) or carbolines, were compared in order to propose fragmentation pathways for each HA. Moreover, labelled AIAs were used to establish the mechanisms. The protonated molecule was always obtained, but subsequent fragmentation was different for both families. In the case of AIAs, major product ions came from the fragmentation of the aminoimidazole moiety, thus the base peak in MS(2) corresponded to the loss of the methyl group, and losses of C(2)NH(3) and CN(2)H(2) were also observed. Further fragmentation occurred in the heterocyclic rings, mainly with losses of HCN and CH(3)CN. For carbolines, the most important product ions came from the loss of ammonia, except for harman and norharman, the loss of a methyl group for methylated carbolines or the loss of diverse fragments from the heterocyclic rings. In some cases, ion-molecule reactions into the ion trap were observed. For instance, for AalphaC or MeAalphaC one ion originating from these reactions corresponded to the base peak.

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Capillary electrophoresis and capillary electrophoresis ‐ electrospray ‐ mass spectroscopy for the analysis of heterocyclic amines

Cited by 28 publications

References 25 publications

Advantages of Pressurization in Capillary Electrophoresis/Electrospray Ionization Mass Spectrometry

Advantages of Pressurization in Capillary Electrophoresis/Electrospray Ionization Mass Spectrometry

In-capillary micro solid-phase extraction and capillary electrophoresis separation of heterocyclic aromatic amines with nanospray mass spectrometric detection

Multistep mass spectrometry of heterocyclic amines in a quadrupole ion trap mass analyser

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