Capillary Electrophoresis

Gordon, Manuel J.; Huang, Xiaoxia; Pentoney, Stephen L.; Zare, Richard N.

doi:10.1126/science.242.4876.224

Cited by 312 publications

(90 citation statements)

References 66 publications

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“…Several types of CD have been proposed [296][297][298][299]. Analytical grade CDs utilizing an AC impedance measurement allow the avoidance of artifacts of sensor surface polarization and interferences from the galvanically coupled HV source.…”

Section: Contact and Contactless Conductivity Detection (Cd Ccd C 4mentioning

confidence: 99%

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

Boudko

2007

Journal of Chromatography B

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This review briefly summarizes recent progress in fundamental understanding and analytical profiling of the L-arginine/nitric oxide (NO) pathway. It focuses on key analytical references of NO actions and on the experimental acquisition of these references in vivo, with capillary electrophoresis (CE) and high-performance capillary electrophoresis (HPCE) comprising one of the most flexible and technologically promising analytical platform for comprehensive high-resolution profiling of NO-related metabolites. Second aim of this review is to express demands and bridge efforts of experimental biologists, medical professionals and chemical analysis-oriented scientists who strive to understand evolution and physiological roles of NO and to develop analytical methods for use in biology and medicine.

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Section: Contact and Contactless Conductivity Detection (Cd Ccd C 4mentioning

confidence: 99%

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

Boudko

2007

Journal of Chromatography B

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“…Capillary electrophoretic methods including open-column zone electrophoresis, disc electrophoresis in gels, isotachophoresis and isoelectric focusing have received considerable attention from the analytical community over the last three or four years (80,81,82). In capillary zone electrophoresis (CZE), nanogram quantities of sample are placed in a silica capillary, 50 to 300 microns in diameter and 50 to 100 cm long.…”

Section: High-resolution Purification Techniques Electrophoresismentioning

confidence: 99%

Modeling and Applications of Downstream Processing

Hamel

Hunter

1990

ACS Symposium Series

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Downstream processing is playing an increasingly important role in the biochemical industry, especially since the advent of recombinant DNA technology. The use of recombinant DNA technology not only enables improvements in the production efficiency of therapeutic and industrial proteins, but it also permits the modification and improvement of protein structure and thus function.However, the commercial application of such technology was initially accompanied by concerns over product safety.Quality criteria have been made especially stringent for products derived from genetically-modified microorganisms. The establishment of strict quality guidelines was the result of early concern about the oncogenic potential related to products contaminated by DNA sequences of the host mammalian cells (1). The quest for high quality has created a growing need for high-resolution techniques at the process scale as well as for novel strategies for the isolation and purification of bioproducts. Since the typical environment for producing biologicals is a complex one and quality criteria need to be strict, primary recovery techniques are typically implemented in a purification scheme prior to (or in conjunction with) high-resolution techniques. The most sophisticated and useful schemes take advantage of both the different physical and chemical properties of the components in complex mixtures and of the interactive nature of the downstream processing techniques (see Figure 1).

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“…One of the most important advancements in 2-D PAGE is the realization of immobilized pH gradients (IPGs) [4], which brings improved reproducibility and higher loading capacity [5]. Capillary electrophoresis (CE) is an easier and more rapid alternative to slab gel electrophoresis [6][7][8][9]. Among the various modes of CE, capillary isoelectric focusing (CIEF) is a powerful method that separates ampholytic substances based on their isoelectric points (pI) [10].…”

Section: Introductionmentioning

confidence: 99%

Repeatedly usable immobilized pH gradient in a monolithic capillary column

Yang¹,

Zhu²,

Zhang³

et al. 2004

Electrophoresis

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Repeatedly usable immobilized pH gradient in a monolithic capillary columnGlycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) were used to synthesize a monolithic capillary column containing reactive epoxy groups. Glutaraldehyde was introduced and linked to the monolith after a process of amination. An aqueous solution of commercial carrier ampholytes (CAs, Ampholine) was focused in such a polymer column. The primary amino groups of CAs reacted with glutaraldehyde along the capillary. CAs were immobilized at different positions in the column according to their isoelectric points (pI), resulting in a monolithic immobilized pH gradient (M-IPG). Isoelectric focusing (IEF) was performed without CAs in such an M-IPG column. Due to the covalent attachment of the CAs this M-IPG can be repeatedly used after its preparation. Good stability, linearity, and reproducibility were obtained.

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Capillary Electrophoresis

Abstract: Electrophoretic separations carried out in capillary tubes offer the possibilities of rapid and automated analyses of small volumes of complex mixtures with unprecedented resolution and sensitivity. Some emerging developments of this new instrumentation are reviewed.

Cited by 312 publications

References 66 publications

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

Modeling and Applications of Downstream Processing

Repeatedly usable immobilized pH gradient in a monolithic capillary column

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