Capillary Electrochromatography with Monolithic Poly(styrene-co-divinylbenzene-co-methacrylic acid) as the Stationary Phase

Abstract

A new kind of monolithic capillary electrochromatography column with poly(styrene-co-divinylbenzene-co-methacrylic acid) as the stationary phase has been developed. The stationary phase was found to be porous by scanning electron microscopy and the composition of the continuous bed was proved by IR spectroscopy to be the ternary polymer of styrene, divinylbenzene, and methacrylic acid. The effects of operating parameters, such as voltage, electrolyte, and organic modifier concentration in the mobile phase on electroosmotic flow were studied systematically. The retention mechanism of neutral solutes on such a column proved to be similar to that of reversedphase high performance liquid chromatography. In addition, fast analyses of phenols, chlorobenzenes, anilines, isomeric compounds of phenylenediamine and alkylbenzenes within 4.5 min were achieved.

Separation of acidic and basic compounds in capillary electrochromatography with polymethacrylate-based monolithic columns

“…As an alternative to the conventional packed columns, monolithic columns have attracted great attention in recent years due to their simple preparation procedure and no need for frits. So far, silica rod [2][3][4], polymethacrylate-based monolith [5][6][7], polyacrylamide monolithic column [8,9] and polystyrene continuous bed [10][11][12] have been fabricated and applied to the separations of peptides [13], proteins [14], steroids [15], polymers [6] and amino acids [16] and so on. Several reviews on in situ polymerization of synthetic organic polymers as monolithic columns for CEC have been published recently [17][18][19].…”

Basic Chromatographic Properties of Polyethylene Glycol-type, Polymer-based Monolithic Columns

“…such as 50 -100 μm because of shrinkage of the polymer. 9,[16][17][18][19][20][21][22][23][24][25] In this experiment, in spite of the wider 0.53 mm i.d. capillary, PEG-type monolithic polymers were formed homogeneously.…”

Recent progress in capillary electrochromatography

“…Another type of polymeric monoliths is based on polystyrene or polymethacrylate polymers [90±92, 107,162,163], which are more rigid in nature. Many different chemistries can be incorporated within the polymeric structure to give a desired selectivity.…”