Capillary-based immunoassays, immunosensors and DNA sensors – steps towards integration and multi-analysis

Mastichiadis, Christos; Niotis, Aristeidis E.; Petrou, Panagiota; Kakabakos, Sotirios; Μισιακός, Κωνσταντίνος

doi:10.1016/j.trac.2008.08.003

Cited by 24 publications

(12 citation statements)

References 65 publications

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“…Die Robustheit von Immunassays kann durch Verwendung neuer Antikörper und die Veränderung der Matrix des Testsystems verbessert werden. [111][112][113][114][115][116][117] Bei der Probenvorbereitung können chromatographische Säulen zur Abtrennung von Störsubstanzen verwendet werden. Die Zellanalytik mithilfe der Durchflusszytometrie wird durch Einführung…”

Section: Forschungen Zu Etablierten Methoden Und Implementierung Von unclassified

Die klinische Chemie – Herausforderung der Medizin für die analytische Chemie und die Nanowissenschaften

Durner

2010

Angewandte Chemie

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Die klinische Chemie und Laboratoriumsmedizin blickt auf eine über 150jährige, ereignisreiche Geschichte zurück. Das Fach repräsentiert einen Querschnitt zu allen medizinischen Disziplinen und den übrigen Naturwissenschaften. Die klinische Chemie zeigt, wie neue Erkenntnisse aus der biochemischen, biologischen, chemisch‐analytischen, technischen und informationstechnologischen Grundlagenforschung zum Nutzen einer verbesserten Diagnostik, Therapiekontrolle und Prävention in den medizinischen Alltag transferiert werden können. Im vorliegenden Aufsatz wird zunächst die Entwicklung der klinischen Chemie dargelegt. Anschließend werden die einzelnen Schritte von der Blutabnahme bis zum interpretierten Laborwert vorgestellt; hierbei werden sowohl Fallstricke als auch das Qualitätsmanagementsystem beschrieben. Der Einzug neuer Methoden und Trends in die medizinische Analytik wird ebenso erörtert wie Chancen und Probleme der personalisierten Medizin.

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Section: Forschungen Zu Etablierten Methoden Und Implementierung Von unclassified

Die klinische Chemie – Herausforderung der Medizin für die analytische Chemie und die Nanowissenschaften

Durner

2010

Angewandte Chemie

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“…[111][112][113][114][115][116][117] Chromatographic columns can be used during the sample preparation for removal of interfering substances. The cell analysis with flow cytometry was extended by the introduction of new markers in leukemia diagnostics, the study of stem cells and precursor cells, as well as the detection of pathological microorganisms in blood.…”

Section: Nanomechanical Cantilever Sensorsmentioning

confidence: 99%

Clinical Chemistry: Challenges for Analytical Chemistry and the Nanosciences from Medicine

Durner

2010

Angew Chem Int Ed

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Clinical chemistry and laboratory medicine can look back over more than 150 years of eventful history. The subject encompasses all the medicinal disciplines as well as the remaining natural sciences. Clinical chemistry demonstrates how new insights from basic research in biochemical, biological, analytical chemical, engineering, and information technology can be transferred into the daily routine of medicine to improve diagnosis, therapeutic monitoring, and prevention. This Review begins with a presentation of the development of clinical chemistry. Individual steps between the drawing of blood and interpretation of laboratory data are then illustrated; here not only are pitfalls described, but so are quality control systems. The introduction of new methods and trends into medicinal analysis is explored, along with opportunities and problems associated with personalized medicine.

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“…In the past decade, capillary electrophoretic immunoassay (CEIA) has been proved to be a powerful technique for the separation and analysis of biological compounds . CEIA can rapidly separate antibody and antigen/antigen–antibody complexes using small amounts of sample and reagent, while requiring highly sensitive detector for the detection of trace analytes from complex biological compound.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive capillary electrophoretic immunoassay of rheumatoid factor in human serum with gold nanoparticles enhanced chemiluminescence detection

et al. 2014

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A new CE-based immunoassay method for the determination of rheumatoid factor was developed using chemiluminescent reaction of luminol and hydrogen peroxide catalyzed by gold nanoparticles (AuNPs). In this method, AuNPs were synthesized and conjugated with anti-RF (antibody, Ab) to form tagged Ab (AuNPs-Ab, Ab*), which subsequently linked to limited amount of RF (antigen, Ag) to produce Ab*-Ag complex by a noncompetitive immunoreaction. AuNPs were used to label antibody and amplify chemiluminescent signal. Under the optimized conditions, the mixture of free Ab* and Ab*-Ag complex was well separated and detected. This method yields a wide linear range of 0.01-20 μg/mL with a correlation coefficient of 0.997, and the detection limit of RF reaches 5.95 ng/mL (ca. 6.0 pmol/L, S/N = 3). The proposed method was successfully applied for the quantification of RF in human sera from patients with rheumatoid arthritis. This highly sensitive and selective method could be developed into a promising and useful technique for biological molecules determination in clinical analysis.

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Capillary-based immunoassays, immunosensors and DNA sensors – steps towards integration and multi-analysis

Cited by 24 publications

References 65 publications

Die klinische Chemie – Herausforderung der Medizin für die analytische Chemie und die Nanowissenschaften

Die klinische Chemie – Herausforderung der Medizin für die analytische Chemie und die Nanowissenschaften

Clinical Chemistry: Challenges for Analytical Chemistry and the Nanosciences from Medicine

Highly sensitive capillary electrophoretic immunoassay of rheumatoid factor in human serum with gold nanoparticles enhanced chemiluminescence detection

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