Capacity To Utilize Raffinose Dictates Pneumococcal Disease Phenotype

Minhas, Vikrant; Harvey, Richard M.; McAllister, Lauren J.; Seemann, Torsten; Syme, Anna; Baines, Sarah L.; Paton, James C.; Trappetti, Claudia

doi:10.1128/mbio.02596-18

Cited by 25 publications

(47 citation statements)

References 36 publications

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“…However, the decline in this tri-saccharide during colonization of P. expansum may indicate the ability of this pathogen to degrade raffinose in order to utilize it as carbon source (Fang and St Leger, 2010). This ability to degrade raffinose may be significant to its virulence, as was previously shown for bacterial pathogens (Minhas et al, 2019). These results are in line with the transcriptomic analysis, which indicated that P. expansum has a significantly higher carbon metabolism than C. gloeosporioides.…”

Section: Effects Of Colletotrichum and Penicillium Colonization On Hosupporting

confidence: 86%

Sugar‐regulated susceptibility of tomato fruit toColletotrichumandPenicilliumrequires differential mechanisms of pathogenicity and fruit responses

Ziv

Kumar

Sela

et al. 2020

Environmental Microbiology

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Summary Colletotrichum gloeosporioides and Penicillium expansum cause postharvest diseases in tropical and deciduous fruit. During colonization, C. gloeosporioides and P. expansum secrete ammonia in hosts with low sugar content (LowSC) and gluconic acid in hosts with high sugar content (HighSC), respectively, as a mechanism to modulate enhanced pathogenicity. We studied the pathogens interactions with tomato lines of similar genetic background but differing in their sugar content. Colletotrichum gloeosporioides showed enhanced colonization of the LowSC line with differential expression response of 15% of its genes including enhanced relative expression of glycosyl hydrolases, glucanase and MFS‐transporter genes. Enhanced colonization of P. expansum occurred in the HighSC line, accompanied by an increase in carbohydrate metabolic processes mainly phosphoenolpyruvate carboxykinase, and only 4% of differentially expressed genes. Gene response of the two host lines strongly differed depending on the sugar level. Limited colonization of HighSC line by C. gloeosporioides was accompanied by a marked alteration of gene expression compared the LowSC response to the same pathogen; while colonization by P. expansum resulted in a similar response of the two different hosts. We suggest that this differential pattern of fungal/host responses may be the basis for the differential of host range of both pathogens in nature.

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Section: Effects Of Colletotrichum and Penicillium Colonization On Hosupporting

confidence: 86%

Sugar‐regulated susceptibility of tomato fruit toColletotrichumandPenicilliumrequires differential mechanisms of pathogenicity and fruit responses

Ziv

Kumar

Sela

et al. 2020

Environmental Microbiology

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“…6B). Interestingly, Spn ear isolates demonstrate a reduced capacity to use the sugar raffinose comparing with blood isolates 61 .…”

Section: Spn Growth Stimulation Is Hemoglobin-dependent and Utilizes mentioning

confidence: 99%

Hemoglobin stimulates vigorous growth of Streptococcus pneumoniae and shapes the pathogen's global transcriptome

Akhter

Womack

Vidal

et al. 2020

Sci Rep

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Streptococcus pneumoniae (Spn) must acquire iron from the host to establish infection. We examined the impact of hemoglobin, the largest iron reservoir in the body, on pneumococcal physiology. Supplementation with hemoglobin allowed Spn to resume growth in an iron-deplete medium. Pneumococcal growth with hemoglobin was unusually robust, exhibiting a prolonged logarithmic growth, higher biomass, and extended viability in both iron-deplete and standard medium. We observed the hemoglobin-dependent response in multiple serotypes, but not with other host proteins, free iron, or heme. Remarkably, hemoglobin induced a sizable transcriptome remodeling, effecting virulence and metabolism in particular genes facilitating host glycoconjugates use. Accordingly, Spn was more adapted to grow on the human α − 1 acid glycoprotein as a sugar source with hemoglobin. A mutant in the hemoglobin/heme-binding protein Spbhp-37 was impaired for growth on heme and hemoglobin iron. The mutant exhibited reduced growth and iron content when grown in THYB and hemoglobin. In summary, the data show that hemoglobin is highly beneficial for Spn cultivation in vitro and suggest that hemoglobin might drive the pathogen adaptation in vivo. The hemoglobin receptor, Spbhp-37, plays a role in mediating the positive influence of hemoglobin. These novel findings provide intriguing insights into pneumococcal interactions with its obligate human host.

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“…In contrast to iCJ415, iDS372 cannot grow on either stachyose or raffinose, which is due to lack of a raffinose degrading reaction (Stachyose is degraded to raffinose). Raffinose utilization by S. pneumoniae has been shown to be an independent predictor for disease spectrum (Minhas et al, 2019).…”

Section: Experimental Verification Of Carbonand Nitrogen Utilization mentioning

confidence: 99%

Reconstruction and Validation of a Genome-Scale Metabolic Model of Streptococcus oralis (iCJ415), a Human Commensal and Opportunistic Pathogen

et al. 2020

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The mitis group of streptococci (MGS) is a member of the healthy human microbiome in the oral cavity and upper respiratory tract. Troublingly, some MGS are able to escape this niche and cause infective endocarditis, a severe and devastating disease. Genome-scale models have been shown to be valuable in investigating metabolism of bacteria. Here we present the first genome-scale model, iCJ415, for Streptococcus oralis SK141. We validated the model using gene essentiality and amino acid auxotrophy data from closely related species. iCJ415 has 71-76% accuracy in predicting gene essentiality and 85% accuracy in predicting amino acid auxotrophy. Further, the phenotype of S. oralis was tested using the Biolog Phenotype microarrays, giving iCJ415 a 82% accuracy in predicting carbon sources. iCJ415 can be used to explore the metabolic differences within the MGS, and to explore the complicated metabolic interactions between different species in the human oral cavity.

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Capacity To Utilize Raffinose Dictates Pneumococcal Disease Phenotype

Cited by 25 publications

References 36 publications

Sugar‐regulated susceptibility of tomato fruit toColletotrichumandPenicilliumrequires differential mechanisms of pathogenicity and fruit responses

Sugar‐regulated susceptibility of tomato fruit toColletotrichumandPenicilliumrequires differential mechanisms of pathogenicity and fruit responses

Hemoglobin stimulates vigorous growth of Streptococcus pneumoniae and shapes the pathogen's global transcriptome

Reconstruction and Validation of a Genome-Scale Metabolic Model of Streptococcus oralis (iCJ415), a Human Commensal and Opportunistic Pathogen

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