Capacitative Ca2+ influx in glial cells is inhibited by glycolytic inhibitors

ATP‐induced, sustained calcium signalling in cultured rat cortical astrocytes: evidence for a non‐capacitative, P2X7‐like‐mediated calcium entry

“…Three main categories of Ca 2+ ‐permeable channels have been reported to mediate Ca 2+ entry from the extracellular space [8]: voltage‐operated Ca 2+ channels, ligand‐gated non‐specific cation channels and receptor‐dependent Ca 2+ channels further subdivided into store‐operated (or capacitative Ca 2+ channels) and non‐capacitative Ca 2+ channels regulated independently of store depletion. The existence of store‐operated channels in astrocytes and glioma cells has been recently demonstrated [10,14,15,23,24]. Wu et al [24] reported that intracellular ATP levels are important for the maintenance of the [Ca 2+ ] i plateau, and Rzigalinski et al [15] suggested that 5,6‐epoxyeicosatrienoic acid is a molecular component of the intracellular signalling cascade coupling store depletion to Ca 2+ entry in cultured cortical astrocytes.…”

“…The existence of store‐operated channels in astrocytes and glioma cells has been recently demonstrated [10,14,15,23,24]. Wu et al [24] reported that intracellular ATP levels are important for the maintenance of the [Ca 2+ ] i plateau, and Rzigalinski et al [15] suggested that 5,6‐epoxyeicosatrienoic acid is a molecular component of the intracellular signalling cascade coupling store depletion to Ca 2+ entry in cultured cortical astrocytes. Moreover, the involvement of non‐capacitative Ca 2+ entry mechanisms was suggested to contribute to the hypoosmolarity‐ and histamine‐induced [Ca 2+ ] i responses in cultured rat astrocytes [10,23].…”

Mold Allergen, Pen c 13, Induces IL-8 Expression in Human Airway Epithelial Cells by Activating Protease-Activated Receptor 1 and 2

“…A549 cells (4 ϫ 10 5 cells) were plated on cover slides in 25-mm plates and intracellular calcium levels measured as described previously (29). In brief, the cells were loaded for 1 h at room temperature with 2 M fura-2 AM (Molecular Probes) in PBS, then a small group of cells (5-10 cells for each experiment) was excited alternately at 340 and 380 nm and emission measured at 510 nm.…”

Regulation of Store-Operated Calcium Entry by Calcium-Independent Phospholipase A2in Rat Cerebellar Astrocytes

“…SOCE can be induced, e.g., in cultured rat cerebellar astrocytes (Jung et al, 2000;Lo et al, 2002) and C6 glioma cells (Wu et al, 1997), in which it is enhanced by either cAMP (Wu et al, 1999) or nitric oxide (Li et al, 2003), and reduced by arachidonic acid (AA) (Sergeeva et al, 2003;Yang et al, 2005). In glial cells in acute brain slices, however, mechanisms of regulating SOCE have not been investigated.…”