Capacitation of Hamster Spermatozoa With Adrenal Gland Extracts

Bavister, Barry D.; Yanagimachi, Ryuzo; Teichman, R.

doi:10.1095/biolreprod14.2.219

Cited by 43 publications

(11 citation statements)

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“…of 4 observations) significantly different from those of respective control without A23187 (P < 005). Effects of tissue extracts on sperm cyclic nucleotide levels Hamster serum and adrenal extracts have been reported to contain a substance of low molecular weight which stimulates hamster sperm motility (Bavister, Yanagimachi & Teichman, 1976). In the present study only sea urchin FRE caused an elevation in sperm cyclic AMP levels (Table 4).…”

Section: Resultsmentioning

confidence: 99%

Effects of factors released from eggs and other agents on cyclic nucleotide concentrations of sea urchin spermatozoa

Garbers¹,

Kopf²

1978

Reproduction

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Factors released from eggs (FRE) of the sea urchin, Strongylocentrotus purpuratus, caused up to 20-fold increases in sperm cyclic AMP levels after a 1-min incubation. Putative cyclic nucleotide phosphodiesterase inhibitors such as theophylline acted in a synergistic manner with FRE to cause even greater increases in sperm cyclic AMP levels. This effect appeared to be specific for egg factors since various hormones (triiodothyronine, norepinephrine, histamine), nucleosides (adenosine, guanosine), nucleophiles (axide), anaesthetics (procaine), ionophores (X537A, A23187), metals (Mn2+) and neurotransmitters (acetylcholine) did not increase sperm cyclic AMP levels. Various mammalian tissue extracts (serum, uterus, adrenal, ovary, lung) also had no effect. We suggest that the activity which elevates the cyclic AMP of sea urchin spermatozoa is specifically associated with sea urchin eggs.

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Section: Resultsmentioning

confidence: 99%

Effects of factors released from eggs and other agents on cyclic nucleotide concentrations of sea urchin spermatozoa

Garbers¹,

Kopf²

1978

Reproduction

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“…Until recently it was impossible to analyze ion requirements for some aspects of the physiology of hamster gametes and fertilization because of the lack of chemically defined media that supported sperm capacitation and fertilization. This is now possible, thanks to the endeavor by Meizel, Bavister, and their associates [Bavister et al, 1976;Cornett and Meizel, 1978;Meizel et al, 1980;Leibfried and Bavister, 19811, who developed semi-chemically defined media that support capacitation and acrosome reaction of spermatozoa as well as fertilization in the hamster. a supporting capacitation and acrosome reaction of spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

Requirement of extracellular calcium ions for various stages of fertilization and fertilization‐related phenomena in the hamster

1982

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Using a semi‐chemically defined medium, the requirement of extracellular Ca2+ for survival, capacitation, and acrosome reaction of spermatozoa as well as various stages of fertilization in the hamster was studied. A Ca2+‐deficient environment is unfavorable for long‐term survival of spermatozoa. Sperm capacitation may occur in Ca2+‐deficient media, but not as efficiently as in normal media. The acrosome reaction definitely requires extracellular Ca2+. Other processes or phenomena that require extracellular Ca2+ are initiation and maintenance of hyperactivated motility of spermatozoa, penetration of acrosome‐reacted spermatozoa into the zona pellucida, fusion of the spermatozoa with eggs, and the development of pronuclear eggs into two‐cell embryos. Extracellular Ca2+ is apparently unnecessary for the attachment of spermatozoa to the zona and egg surfaces, decondensation of the sperm nucleus, and the development of sperm and egg pronuclei within the egg. These results were compared with data obtained in other species such as the sea urchin, mouse, rat and guinea pig.

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“…Recently, it has been reported that the dialyzable factor can be replaced by a "motility factor" of less than 1000 molecular weight obtained from extracts of hamster adrenal gland (10) or hamster, guinea pig, or human spermatozoa (11,12). The motility factor derived from spermatozoa also seems to maintain hamster sperm viability upon dilution in vitro (12).…”

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confidence: 99%

Stimulation of in vitro activation and the acrosome reaction of hamster spermatozoa by catecholamines

Cornett

Meizel

1978

Proc. Natl. Acad. Sci. U.S.A.

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Capacitation and the acrosome reaction of mammalian spermatozoa are essential for fertilization. In vitro results are presented that demonstrate that catecholamines stimulate activation (a whiplash flagellar movement characteristic of capacitated hamster spermatozoa) and the acrosome reaction. Protein-free ultrafiltrates of bovine adrenal cortex and medulla preparations stimulated motility, activation, and acrosome reactions of hamster spermatozoa in the presence of bovine serum albumin. The medulla preparation was more effective than the cortex preparation in the stimulation of activation and acrosome reactions. Epinephrine (0.5-50 paM) and norepinephrine (50.0 pM) in the presence of bovine serum albumin and a partially purified protein-free cortex preparation also stimulated activation and the acrosome reactions. Both activation and acrosome reactions in the presence of epinephrine were inhibited by the adrenergic antagonists phentolamine and propranolol, suggesting the involvement of a-and a-adrenergic receptors in the stimulation of capacitation and the acrosome reaction. In addition, phenylephrine, an a-adrenergic agonist, was as potent as epinephrine in the stimulation of acrosome reactions, but activation was reduced. Isoproterenol, a P-adrenergic agonist, was as potent as epinephrine in the stimulation of activation, but acrosome reactions were reduced. High percentages of both activation and acrosome reactions were observed only in the presence of epinephrine, norepinephrine, or phenylephrine and isoproterenol together.Sperm capacitation (subtle cellular changes occurring within the female reproductive tract or in vitro) and the resulting acrosome reaction (a fusion and vesiculation of the outer acrosomal membrane and its overlying sperm head plasma membrane) are essential for mammalian fertilization (1, 2). The molecular events of capacitation and of the acrosome reaction, including its initiation, are only partially understood (3). Capacitation and the acrosome reaction can be induced in hamster spermatozoa in vitro by detoxified follicular fluid (4,5) or by blood sera (6). A nondialyzable serum or follicular fluid factor (labile at 900) was required for the acrosome reaction to occur and a dialyzable heat-stable factor derived from serum or follicular fluid was necessary for sperm motility, activation (the whiplash flagellar movement characteristic of capacitated hamster spermatozoa), and the occurrence of the optimum number of acrosome reactions (4-6). It has now been shown that certain serum albumins can replace the nondialyzable factor (6-9) and that the bovine follicular fluid factor is serum albumin (9).Recently, it has been reported that the dialyzable factor can be replaced by a "motility factor" of less than 1000 molecular weight obtained from extracts of hamster adrenal gland (10) or hamster, guinea pig, or human spermatozoa (11,12). The motility factor derived from spermatozoa also seems to maintain hamster sperm viability upon dilution in vitro (12). In the present paper the bovin...

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Capacitation of Hamster Spermatozoa With Adrenal Gland Extracts

Abstract: The hamster adrenal gland was found to be a new and very potent source of "sperm motility factor" (SMF); the activity of SMF in adrenal extracts was approximately 100 to 200 times that in hamster blood serum.Hamster

Cited by 43 publications

References 0 publications

Effects of factors released from eggs and other agents on cyclic nucleotide concentrations of sea urchin spermatozoa

Effects of factors released from eggs and other agents on cyclic nucleotide concentrations of sea urchin spermatozoa

Requirement of extracellular calcium ions for various stages of fertilization and fertilization‐related phenomena in the hamster

Stimulation of in vitro activation and the acrosome reaction of hamster spermatozoa by catecholamines

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