Capacitation-like changes occur in mouse spermatozoa cooled to low temperatures

Fuller, Sebastian; Whittingham, D. G.

doi:10.1002/(sici)1098-2795(199703)46:3<318::aid-mrd10>3.0.co;2-v

Cited by 42 publications

(10 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nonetheless, if the sperm maturational events are not given time to occur prior to zona hardening, a reduced number of 2-cell embryos or a complete fertilization block would be observed. The need to incubate cryopreserved C57BL/6 sperm is in contrast to previous studies, in which cooling and cryopreservation reduced the time necessary for sperm to obtain fertilization competency [9,18]. Conceivably, inherent genetic components regulate the differences observed in this phenotype.…”

Section: Promoting/maintaining Fertilization Capacitymentioning

confidence: 91%

Conserving, Distributing and Managing Genetically Modified Mouse Lines by Sperm Cryopreservation

Santos¹

2011

Genetic Engineering

View full text Add to dashboard Cite

Background: Sperm from C57BL/6 mice are difficult to cryopreserve and recover. Yet, the majority of genetically modified (GM) lines are maintained on this genetic background. Methodology/Principal Findings: Reported here is the development of an easily implemented method that consistently yields fertilization rates of 7065% with this strain. This six-fold increase is achieved by collecting sperm from the vas deferens and epididymis into a cryoprotective medium of 18% raffinose (w/v), 3% skim milk (w/v) and 477 mM monothioglycerol. The sperm suspension is loaded into 0.25 mL French straws and cooled at 3761uC/min before being plunged and then stored in LN 2. Subsequent to storage, the sperm are warmed at 2,2326162uC/min and incubated in in vitro fertilization media for an hour prior to the addition of oocyte cumulus masses from superovulated females. Sperm from 735 GM mouse lines on 12 common genetic backgrounds including C57BL/6J, BALB/cJ, 129S1/SvImJ, FVB/NJ and NOD/ ShiLtJ were cryopreserved and recovered. C57BL/6J and BALB/cByJ fertilization rates, using frozen sperm, were slightly reduced compared to rates involving fresh sperm; fertilization rates using fresh or frozen sperm were equivalent in all other lines. Developmental capacity of embryos produced using cryopreserved sperm was equivalent, or superior to, cryopreserved IVF-derived embryos. Conclusions/Significance: Combined, these results demonstrate the broad applicability of our approach as an economical and efficient option for archiving and distributing mice.

show abstract

Section: Promoting/maintaining Fertilization Capacitymentioning

confidence: 91%

Conserving, Distributing and Managing Genetically Modified Mouse Lines by Sperm Cryopreservation

Santos¹

2011

Genetic Engineering

View full text Add to dashboard Cite

show abstract

“…; Mazur and Koshimoto ). The sublethal damages leading to reduced lifespan of sperm have been termed capacitation‐like changes (Fuller and Whittingham ; Bravo et al. ) or, more recently, apoptotic‐like changes (Ortega‐Ferrusola et al.…”

Section: The Impact Of Sperm Technologies On Mitochondriamentioning

confidence: 99%

“…Sperm death during cryopreservation is due primarily to osmotic stress (Willoughby et al 1996;Mazur and Koshimoto 2002). The sublethal damages leading to reduced lifespan of sperm have been termed capacitation-like changes (Fuller and Whittingham 1997;Bravo et al 2005) or, more recently, apoptotic-like changes (Ortega-Ferrusola et al 2008). In any case, cryopreserved sperm experiences accelerated ageing and premature senescence.…”

Section: The Impact Of Sperm Technologies On Mitochondriamentioning

confidence: 99%

The Impact of Reproductive Technologies on Stallion Mitochondrial Function

Peña

Dávila

Ball

et al. 2015

Reprod Domestic Animals

View full text Add to dashboard Cite

The traditional assessment of stallion sperm comprises evaluation of sperm motility and membrane integrity and identification of abnormal morphology of the spermatozoa. More recently, the progressive introduction of flow cytometry is increasing the number of tests available. However, compared with other sperm structures and functions, the evaluation of mitochondria has received less attention in stallion andrology. Recent research indicates that sperm mitochondria are key structures in sperm function suffering major changes during biotechnological procedures such as cryopreservation. In this paper, mitochondrial structure and function will be reviewed in the stallion, when possible specific stallion studies will be discussed, and general findings on mammalian mitochondrial function will be argued when relevant. Especial emphasis will be put on their role as source of reactive oxygen species and in their role regulating sperm lifespan, a possible target to investigate with the aim to improve the quality of frozen-thawed stallion sperm. Later on, the impact of current sperm technologies, principally cryopreservation, on mitochondrial function will be discussed pointing out novel areas of research interest with high potential to improve current sperm technologies.

show abstract

“…1 In some species (bull, human, mouse, ram, Siberian tiger) chilled, rewarmed or frozen thawed spermatozoa are able to penetrate the oocyte in a short time after rewarming, while for fresh semen several hours of pre-incubation are needed. [2][3][4][5][6][7][8] Cooling/freezing/thawing of semen is routinely performed in cattle breeding industry in order to perform artificial insemination (AI). These procedures are known to produce reactive oxygen species (ROS) in the sperm samples.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant effect of Mn2+ on capacitation and acrosome reaction of fresh and chilled cattle bull semen

2012

View full text Add to dashboard Cite

The aim of this paper was to investigate the antioxidant effect of Mn 2+ (200 mM) on the sperm capacitation and acrosome reaction of fresh and chilled cattle bull semen. It has been found that Mn 2+ supplementation improves (P≤0.05) the motility at 0, 2, 4 and 6 h of incubation. MDA (malondialdehyde), end product of lipid peroxidation, decreases significantly (P≤0.05) with the supplementation of manganese at 0-and 6-hr of incubation both in fresh and chilled semen. Manganese also increases acrosome reaction significantly (P≤0.05) both in fresh and chilled semen at 0, 4 and 6 h of incubation. Therefore, our findings suggest the role of Mn 2+ supplementation in improving the quality of cattle bull semen by its scavenging property i.e. reduction in the production of reactive oxygen species during its storage at 4°C or incubation at 37°C for capacitation.

show abstract

Capacitation-like changes occur in mouse spermatozoa cooled to low temperatures

Cited by 42 publications

References 34 publications

Conserving, Distributing and Managing Genetically Modified Mouse Lines by Sperm Cryopreservation

Conserving, Distributing and Managing Genetically Modified Mouse Lines by Sperm Cryopreservation

The Impact of Reproductive Technologies on Stallion Mitochondrial Function

Antioxidant effect of Mn2+ on capacitation and acrosome reaction of fresh and chilled cattle bull semen

Contact Info

Product

Resources

About