Capacitance of bilayers in the presence of lipophilic ions

Pickar, Arnold D.; Brown, William C.

doi:10.1016/0005-2736(83)90104-9

Cited by 7 publications

(8 citation statements)

References 10 publications

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“…We use hydrophobic anions and cations in this study to detect changes of membrane properties. Previous work (Andersen and Fuchs, 1975; Bruner, 1975; Benz et al, 1976; Pickar and Brown, 1983) provides a firm basis to understand their function in membranes, including biological membranes (Chanda et al, 2005). DPA was used previously as a novel means to “amplify” capacitive signals associated with exocytosis (Oberhauser and Fernandez, 1995).…”

Section: Methodsmentioning

confidence: 99%

Mechanistic analysis of massive endocytosis in relation to functionally defined surface membrane domains

Hilgemann

Fine

2011

Journal of General Physiology

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A large fraction of endocytosis in eukaryotic cells occurs without adaptors or dynamins. Here, we present evidence for the involvement of lipid domains in massive endocytosis (MEND) activated by both large Ca transients and amphipathic compounds in baby hamster kidney and HEK293 cells. First, we demonstrate functional coupling of the two MEND types. Ca transients can strongly facilitate detergent-activated MEND. Conversely, an amphipath with dual alkyl chains, ditridecylphthalate, is without effect in the absence of Ca transients but induces MEND to occur within seconds during Ca transients. Ca transients, like amphipaths, enhance the extraction of lipids from cells by β-cyclodextrins. Second, we demonstrate that electrical and/or optical signals generated by selected membrane probes are nearly insensitive to MEND, suggesting that those probes segregate into membrane domains that are not taken up by MEND. Triphenylphosphoniums are increasingly excluded from domains that internalize as the carbon chain length increases from 4 to 12. The small cationic membrane dye, FM 4–64, binds well to domains that internalize, whereas a closely related dye with a larger hydrophobic moiety, di-4-ANEPPDHQ (ANEPPDHQ) is excluded. Multiple carrier-type ionophores and a small amphipathic anion, niflumic acid, are also excluded. Probes with modest MEND sensitivity include the hydrophobic anion, dipicrylamine, carbonyl cyanide m-chlorophenylhydrazone, and NBD-phosphatidylethanolamine. Third, we demonstrate that large Ca transients can strongly enhance the extracellular binding of several membrane probes, monitored electrically or optically, consistent with a more disordered membrane with more amphipath-binding sites. Fluorescence shifts of ANEPPDHQ report increased disorder of the extracellular monolayer after large Ca transients, consistent with an increased propensity of the membrane to phase separate and vesiculate. Collectively, the results indicate that >50% of the outer monolayer is ordered and can be selectively internalized during MEND responses initiated by two very different cell perturbations.

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Section: Methodsmentioning

confidence: 99%

Mechanistic analysis of massive endocytosis in relation to functionally defined surface membrane domains

Hilgemann

Fine

2011

Journal of General Physiology

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“…The conductance and capacitance of BLMs were increased by the lipophilic ions, and the dynamic electric properties obtained by charge-pulse experiments showed fast and slow relaxation processes that correspond to charging of the "geometric" membrane and to translocation of mobile ions in the membranes, respectively. The slow relaxation was alternatively measured as low-frequency (LF) dielectric dispersion by impedance (or dielectric) spectroscopy [6].…”

Section: Introductionmentioning

confidence: 99%

Dielectric properties of dipicrylamine-doped erythrocytes, cultured cells and lipid vesicles

Asami

2013

Bioelectrochemistry

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Horse erythrocytes, murine lymphoblasts (L5178Y) and lipid vesicles that were treated with dipicrylamine (DPA) as a lipophilic ion were studied by dielectric spectroscopy over a frequency range of 10 Hz to 10 MHz. The DPA-treated cells and lipid vesicles showed low-frequency (LF) dielectric dispersion around 1-10 kHz in addition to β-dispersion due to the Maxwell-Wagner effect. The LF dispersion corresponds to that found in previous electrorotation (ROT) studies on DPA-treated cells, being due to the translocation of mobile ions in the plasma membranes. Analysis of the LF dispersion based on the mobile charge model provided the area-specific concentration Nt of DPA ions adsorbed at the membrane interfaces and their translocation rate constant ki between the interfaces. The values of Nt and ki were respectively 13-21 nmol/m(2) and 0.7-1.6×10(4) s(-1) for both horse erythrocytes and L5178Y cells at 10 μM DPA, being consistent with those determined by ROT for human erythrocytes and cultured cells.

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“…The chloride carrier in V. utricularis and the chloride pump in A. mediterranea have been shown to increase the apparent specific capacity of the cell membrane. Similarly, absorbed lipophilic ions increase the specific capacity of artificial and biological membranes (Benz and Conti, 1981;Pickar and Brown, 1983). Furthermore, transport systems are able to increase the specific conductance of lipid bilayer membranes and are probably in certain cases responsible for the much higher specific conductance of biological membranes as compared with that of artificial membranes (Lauger et al, 1981).…”

Section: Introductionmentioning

confidence: 99%

Contribution of electrogenic ion transport to impedance of the algae Valonia utricularis and artificial membranes

Wang

Zimmermann

Benz

1994

Biophysical Journal

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The cell membrane of Valonia utricularis contains an electrogenic carrier system for chloride (Wang et al., Biophys J. 59:235-248 (1991)). The electrical impedance of V. utricularis was measured in the frequency range between 1 Hz and 50 kHz. The analysis of the impedance spectra from V. utricularis and its comparison with equivalent circuit models showed that the transport system created a characteristic contribution to the impedance in the frequency range between 10 Hz and 5 kHz. The fit of the impedance spectra with the formalism derived from the theory of carrier-mediated transport allowed the determination of the kinetic parameters of chloride transport through the cell membrane of V. utricularis, and its passive electrical properties. Simultaneous measurements of the kinetic parameters with the charge pulse method demonstrated the equivalence of both experimental approaches with respect to the evaluation of the translocation rate constants of the free and the charged carriers and the total density of carriers within the membrane. Moreover, the impedance spectra of the protonophor-mediated proton transport by FCCP (carbonylcyanide p-trifluoromethoxyphenyl-hydrazone) were measured in model membranes. The carrier system made a substantial contribution to the impedance of the artificial membranes. The analysis of the spectra in terms of a simple carrier system (Benz and McLaughlin, 1983, Biophys. J. 41:381-398) allowed the evaluation of the kinetic and equilibrium parameters of the FCCP-mediated proton transport. The possible application of the measurement of impedance spectra for the study of biological transport systems is discussed.

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Capacitance of bilayers in the presence of lipophilic ions

Cited by 7 publications

References 10 publications

Mechanistic analysis of massive endocytosis in relation to functionally defined surface membrane domains

Mechanistic analysis of massive endocytosis in relation to functionally defined surface membrane domains

Dielectric properties of dipicrylamine-doped erythrocytes, cultured cells and lipid vesicles

Contribution of electrogenic ion transport to impedance of the algae Valonia utricularis and artificial membranes

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