Capability of exopolysaccharide-producing Lactobacillus paraplantarum BGCG11 and its non-producing isogenic strain NB1, to counteract the effect of enteropathogens upon the epithelial cell line HT29-MTX

Živković, Milica; Hidalgo-Cantabrana, Claudio; Kojić, Milan; Gueimonde, Miguel; Golić, Nataša; Ruas‐Madiedo, Patricia

doi:10.1016/j.foodres.2015.05.012

Cited by 33 publications

(27 citation statements)

References 41 publications

(36 reference statements)

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“…In our study, the results of a SEC-MALLS analysis revealed that insertional mutagenesis of the glps_2198 gene, encoding priming glycosyltransferase, led to the absence of the P2 fraction in EPS7, related to low molecular weight EPS-SJ (13,600 Da) present in BGSJ2-83, as well as to an increase in molecular weight of the P1 and its reduced production. According to literature data the gene encoding priming glycosyltransferase is highly evolutionarily conserved ( Jolly and Stingele, 2001 ) and could be located at the beginning of the eps operons, as reported for L. helveticus NCC2745 ( Jolly et al, 2002 ) and L. paraplantarum BGCG11 ( Zivkovic et al, 2015 ), in the middle of the eps clusters, as in Streptococcus thermophilus strains ( Stingele et al, 1996 ; Almiron-Roig et al, 2000 ), L. lactis NIZO B40 ( Kleerebezem et al, 1999 ), and L. delbrueckii subsp. bulgaricus Lfi5 ( Lamothe et al, 2002 ), or in the vicinity of the end of the eps clusters as in the case of L. rhamnosus ( Péant et al, 2005 ) and here in the case of BGSJ2-8.…”

Section: Discussionmentioning

confidence: 83%

“…In this study we have shown that EPS-SJ-producing strain BGSJ2-83 exhibits higher adhesion to Caco-2 cells and is more capable of competing for the colonization of the intestinal niche than E. coli strain ATCC25922. Similarly, non-EPS producing derivative of EPS-CG11 (NB1) more efficiently reduced the association of Clostridium difficile or Yersinia enterocolitica than parental BGCG11 strain ( Zivkovic et al, 2015 ). Interestingly, the presence of EPS-CG11-producing strain BGCG11, exhibiting low adhesion to Caco-2 cells, decreased the association of E. coli ATCC25922 to Caco-2 cells, similarly to previously obtained results by Zivkovic et al (2015) .…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, non-EPS producing derivative of EPS-CG11 (NB1) more efficiently reduced the association of Clostridium difficile or Yersinia enterocolitica than parental BGCG11 strain ( Zivkovic et al, 2015 ). Interestingly, the presence of EPS-CG11-producing strain BGCG11, exhibiting low adhesion to Caco-2 cells, decreased the association of E. coli ATCC25922 to Caco-2 cells, similarly to previously obtained results by Zivkovic et al (2015) . Hence, it could be concluded that both EPS-CG11 and EPS-SJ are effective macromolecules protecting the IEC from pathogen binding regardless of their adhesion ability ( Zivkovic et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…Generally, the EPS biodiversity could be result of different sugar nucleotide biosynthesis pathways and genetic variability of the strains ( Mozzi et al, 2006 ). The genetic elements involved in the synthesis of the conserved structure of the polymer are organized into operons, located on chromosomal or plasmid DNA ( Boels et al, 2001 ; Zivkovic et al, 2015 ). The genes present in the eps operons encode proteins with specific functions: assembling of repeating units, chain length determination, polymerization, export, and regulation ( de Vuyst and Degeest, 1999 ).…”

Section: Introductionmentioning

confidence: 99%

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EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp. paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E. coli Association to Caco-2 Cells

et al. 2016

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The aim of this study was to determine the role of an exopolysaccharide produced by natural dairy isolate Lactobacillus paracasei subsp. paracasei BGSJ2-8, in the adhesion to intestinal epithelial cells and a decrease in Escherichia coli’s association with Caco-2 cells. Annotation of the BGSJ2-8 genome showed the presence of a gene cluster, epsSJ, which encodes the biosynthesis of the strain-specific exopolysaccharide EPS-SJ, detected as two fractions (P1 and P2) by size exclusion chromatography (SEC) coupled with multi-angle laser light scattering (MALLS) detection. SEC-MALLS analysis revealed that an EPS-SJ- mutant (EPS7, obtained by insertion mutagenesis of the glps_2198 gene encoding primary glycosyltransferase) does not produce the P2 fraction of EPS-SJ. Transmission electron microscopy showed that EPS7 mutant has a thinner cell wall compared to the EPS-SJ+ strain BGSJ2-83 (a plasmid free-derivative of BGSJ2-8). Interestingly, strain BGSJ2-83 showed higher adhesion to Caco-2 epithelial intestinal cell line than the EPS7 mutant. Accordingly, BGSJ2-83 effectively reduced E. coli ATCC25922’s association with Caco-2 cells, while EPS7 did not show statistically significant differences. In addition, the effect of EPS-SJ on the proliferation of lymphocytes in gastrointestinal associated lymphoid tissue (GALT) was tested and the results showed that the reduction of GALT lymphocyte proliferation was higher by BGSJ2-83 than by the mutant. To the best of our knowledge this is the first report indicating that the presence of EPS (EPS-SJ) on the surface of lactobacilli can improve communication between bacteria and intestinal epithelium, implying its possible role in gut colonization.

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp. paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E. coli Association to Caco-2 Cells

et al. 2016

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“…mental conditions, both outside and inside the host (1,3,4), and in the case of pathogens such as Streptococcus pneumoniae, they can have an important association with immune evasion and virulence (5). EPS can have immunomodulatory and protective properties in the host (6)(7)(8)(9) and can affect the composition and function of the gut microbiota (10,11). EPS can also play a crucial role in biofilm formation, adhesion to host cells, and colonization (3,(12)(13)(14)(15).…”

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confidence: 99%

Identification of Genes Required for Glucan Exopolysaccharide Production in Lactobacillus johnsonii Suggests a Novel Biosynthesis Mechanism

Mayer

D’Amato

Colquhoun

et al. 2020

Appl Environ Microbiol

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Lactobacillus johnsonii FI9785 makes two capsular exopolysaccharides-a heteropolysaccharide (EPS2) encoded by the eps operon and a branched glucan homopolysaccharide (EPS1). The homopolysaccharide is synthesized in the absence of sucrose, and there are no typical glucansucrase genes in the genome. Quantitative proteomics was used to compare the wild type to a mutant where EPS production was reduced to attempt to identify proteins associated with EPS1 biosynthesis. A putative bactoprenol glycosyltransferase, FI9785_242 (242), was less abundant in the Δeps_cluster mutant strain than in the wild type. Nuclear magnetic resonance (NMR) analysis of isolated EPS showed that deletion of the FI9785_242 gene (242) prevented the accumulation of EPS1, without affecting EPS2 synthesis, while plasmid complementation restored EPS1 production. The deletion of 242 also produced a slow-growth phenotype, which could be rescued by complementation. 242 shows amino acid homology to bactoprenol glycosyltransferase GtrB, involved in O-antigen glycosylation, while in silico analysis of the neighboring gene 241 suggested that it encodes a putative flippase with homology to the GtrA superfamily. Deletion of 241 also prevented production of EPS1 and again caused a slow-growth phenotype, while plasmid complementation reinstated EPS1 synthesis. Both genes are highly conserved in L. johnsonii strains isolated from different environments. These results suggest that there may be a novel mechanism for homopolysaccharide synthesis in the Gram-positive L. johnsonii. IMPORTANCE Exopolysaccharides are key components of the surfaces of their bacterial producers, contributing to protection, microbial and host interactions, and even virulence. They also have significant applications in industry, and understanding their biosynthetic mechanisms may allow improved production of novel and valuable polymers. Four categories of bacterial exopolysaccharide biosynthesis have been described in detail, but novel enzymes and glycosylation mechanisms are still being described. Our findings that a putative bactoprenol glycosyltransferase and flippase are essential to homopolysaccharide biosynthesis in Lactobacillus johnsonii FI9785 indicate that there may be an alternative mechanism of glucan biosynthesis to the glucansucrase pathway. Disturbance of this synthesis leads to a slowgrowth phenotype. Further elucidation of this biosynthesis may give insight into exopolysaccharide production and its impact on the bacterial cell.

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Postbiotics: Enhancing human health with a novel concept

Da,

Sun,

et al. 2024

eFood

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Postbiotics is a novel category of biotics that encompasses dead cells, metabolites, and derivatives from gut microbiota. These substances can exhibit their effects without containing cells, such as in cell‐free supernatants and spent media. Examples of widely studied postbiotics include short‐chain fatty acids, exopolysaccharides, cell wall fragments, and bacterial lysates. Evidence suggests that postbiotics have numerous benefits for human health, including antiaging, and anti‐inflammatory properties, and participation in immune and lipid metabolism regulation. Specifically, postbiotics can improve intestinal health by strengthening the intestinal barrier, reducing inflammation, and promoting antibacterial activity. These findings may pave the way for the development of postbiotic functional foods in the future. This review provides an overview of the components, health effects, and industrial applications of postbiotics, aiming to consolidate existing research conclusions and serve as a reference for future studies.

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Capability of exopolysaccharide-producing Lactobacillus paraplantarum BGCG11 and its non-producing isogenic strain NB1, to counteract the effect of enteropathogens upon the epithelial cell line HT29-MTX

Abstract: 1BGCG11 and its non-producing isogenic strain NB1, to counteract the effect of 2 enteropathogens upon the epithelial cell line HT29-MTX

Cited by 33 publications

References 41 publications

EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp. paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E. coli Association to Caco-2 Cells

EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp. paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E. coli Association to Caco-2 Cells

Identification of Genes Required for Glucan Exopolysaccharide Production in Lactobacillus johnsonii Suggests a Novel Biosynthesis Mechanism

Postbiotics: Enhancing human health with a novel concept

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