Cantharidin suppresses gastric cancer cell migration/invasion by inhibiting the PI3K/Akt signaling pathway via CCAT1

Song, Mengyun; Wang, Xianfei; Luo, Yang; Liu, Zilin; Tan, Wanlong; Ye, Pengcheng; Fu, Zhiming; Lu, Fei; Xiang, Wanping; Tang, Lixia; Lin, Yu; Nie, Yongzhan; Xiao, Jiangwei

doi:10.1016/j.cbi.2020.108939

Cited by 35 publications

(17 citation statements)

References 28 publications

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“…Epigenetic silencing of ubiquitin-specific protease 4 by snail1 contributes to macrophage-dependent inflammation and therapeutic resistance in lung cancer [34]. KEGG pathway analysis showed that the network was enriched in the PI3K-Akt signalling pathway, cell cycle and FoxO signalling, which have been confirmed by a large number of studies to participate in cancer progression [35][36][37][38]. These results suggest that the circRNAs in this network may play a key role in the occurrence and development of osteosarcoma.…”

Section: Discussionmentioning

confidence: 67%

Construction of a circRNA-miRNA-mRNA network based on competitive endogenous RNA reveals the function of circRNAs in osteosarcoma

Qiu

Chen

et al. 2020

Cancer Cell Int

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Background: Osteosarcoma (OS) is a common primary malignant bone tumour. Growing evidence suggests that circular RNAs (circRNAs) are closely related to the development of tumours. However, the function of circRNAs in OS remains unknown. Here, we aimed to determine the regulatory mechanisms of circRNAs in OS. Methods:The expression profiles of OS circRNA (GSE96964), microRNA (GSE65071) and mRNA (GSE33382) were downloaded from the Gene Expression Omnibus (GEO) database to identify differentially expressed circRNAs, miRNAs and mRNAs in OS. A ceRNA network was constructed based on circRNA-miRNA pairs and miRNA-mRNA pairs. MRNAs with significant prognostic differences were identified by the TARGET database in the network. Functional and pathway enrichment analyses were performed, and interactions between proteins were predicted using Cytoscape. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to elucidate the possible functions of these differentially expressed circRNAs. Results:A total of 15 downregulated circRNAs, 136 upregulated miRNAs and 52 downregulated mRNAs were identified in OS. Finally, a circRNA-miRNA-mRNA network was constructed in OS based on 14 circRNAs, 24 miRNAs, and 52 mRNAs. GO and KEGG pathway analyses suggested that the mRNAs in the network may be involved in the pathogenesis and progression of OS. Four mRNAs identified by the TARGET database were significantly associated with OS survival prognosis. A circRNA-miRNA-mRNA subnetwork was constructed based on these four mRNAs. Conclusion:Our results provide a deeper understanding of the regulatory mechanisms by which circRNAs compete for endogenous RNAs in OS.

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Section: Discussionmentioning

confidence: 67%

Construction of a circRNA-miRNA-mRNA network based on competitive endogenous RNA reveals the function of circRNAs in osteosarcoma

Qiu

Chen

et al. 2020

Cancer Cell Int

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“…Hsa_circ_0010882 also activates PI3K/ Akt signaling and promotes gastric cancer development 25 . Previous studies p‐PI3K can activate Akt to form p‐Akt, and then participate in the process of gastric cancer development and development 26‐30 . It was shown p‐PI3K and p‐Akt were reduced after Herceptin treatment in gastric cancer cells, and hsa_circ_0000520 overexpression could restrain p‐PI3K and p‐Akt level.…”

Section: Discussionmentioning

confidence: 91%

hsa_circ_0000520 influences herceptin resistance in gastric cancer cells through PI3K‐Akt signaling pathway

Wang

et al. 2020

Clinical Laboratory Analysis

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Background To investigate whether hsa_circ_0000520 affects Herceptin resistance in gastric cancer by regulating the PI3K‐AKT signaling. Methods The expression of hsa_circ_0000520 was detected by qRT‐PCR in gastric cancer tissues and cell lines. A Herceptin‐resistant gastric cancer cell was established. PcDNA and pcDNA‐hsa_circ_0000520 were transfected into NCI‐N87R cells and treated with Herceptin at a concentration of 10 μg/mL for 24 hours. MTT tested cell proliferation, and apoptosis was measured by flow cytometry. IGF‐1 treatment was used to activate PI3K‐Akt signaling. The expression levels of related proteins were detected. Results The expression of hsa_circ_0000520 was reduced in gastric cancer tissues and cell lines, and hsa_circ_0000520 in NCI‐N87R cells was significantly lower than that of NCI‐N87 cells. Compared with the CON group, the cell viability of the Herceptin group was significantly reduced, the apoptosis rate was significantly increased, the level of Bax protein was significantly increased, and the levels of Bcl‐2, p‐PI3K, and p‐Akt protein were significantly reduced. Compared with the Herceptin + pcDNA group, the cell viability of the Herceptin + hsa_circ_0000520 group was significantly reduced, the apoptosis rate was significantly increased, the level of Bax protein was significantly increased, and the levels of p‐PI3K and p‐Akt proteins were significantly reduced. After IGF‐1 treatment, the cell viability was significantly increased, the apoptosis rate was significantly reduced, the level of Bax protein was significantly reduced, and the level of Bcl‐2 protein was significantly increased. Conclusion Hsa_circ_0000520 overexpression may reverse the Herceptin resistance of gastric cancer cells by inhibiting the PI3K‐Akt signaling pathway.

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“…eMT can significantly enhance the migratory ability of cancer cells, including breast cancer cells, which can lead to tumor migration and invasion (9); therefore, drugs targeting the eMT pathway may reduce mortality in patients with breast cancer (37). in recent years, a number of studies have reported that traditional Chinese medicine displays significant anticancer effects (38)(39)(40). aM, which is an important component of the qi-supplementing formula, is widely used in china (41) and the main extract of aM is aPS.…”

Section: Discussionmentioning

confidence: 99%

Astragalus polysaccharide inhibits breast cancer cell migration and invasion by regulating epithelial‑mesenchymal transition via the Wnt/β‑catenin signaling pathway

Yang

Sun

et al. 2020

Mol Med Report

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epithelial-mesenchymal transition (eMT) serves an important role in tumor migration and invasion. Astragalus polysaccharide (aPS), which is the main component of the traditional chinese medicine Astragalus membranaceus, has been identified to display an antitumor effect. However, the effects and mechanisms of aPS during breast cancer migration and invasion are not completely understood. The present study investigated whether aPS inhibited breast cancer migration and invasion by modulating the eMT pathway. an MTT assay and a Ki67 immunofluorescence staining assay demonstrated that aPS inhibited the proliferation of breast cancer cells. The results of the wound healing and Transwell Matrigel invasion assays suggested that aPS decreased the migration and invasion of breast cancer cells. The western blotting and immunofluorescence analyses further demonstrated that aPS had a regulatory effect on eMT-related molecules. aPS decreased the expression levels of Snail and vimentin, but increased e-cadherin expression. aPS also downregulated Wnt1, β-catenin and downstream target expression. additionally, the present results suggested that aPS decreased the proliferation, and eMT-mediated migration and invasion of cells by inhibiting the Wnt/β-catenin signaling pathway. The present study suggested that aPS may serve as a promising therapeutic agent for breast cancer.

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Cantharidin suppresses gastric cancer cell migration/invasion by inhibiting the PI3K/Akt signaling pathway via CCAT1

Cited by 35 publications

References 28 publications

Construction of a circRNA-miRNA-mRNA network based on competitive endogenous RNA reveals the function of circRNAs in osteosarcoma

Construction of a circRNA-miRNA-mRNA network based on competitive endogenous RNA reveals the function of circRNAs in osteosarcoma

hsa_circ_0000520 influences herceptin resistance in gastric cancer cells through PI3K‐Akt signaling pathway

Astragalus polysaccharide inhibits breast cancer cell migration and invasion by regulating epithelial‑mesenchymal transition via the Wnt/β‑catenin signaling pathway

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