The in-season estimation of crop stresses which have the potential of adversely affecting crop yield and/or quality could allow producers to make in-season management decisions to correct for the particular stress. A field study was conducted to evaluate the use of multispectral imagery for estimating corn (Zea mays L.) grain yield, in-season biomass and nitrogen (N) concentration under varying N and drought stresses. The experiment was a split-plot design with three replications using a factorial arrangement of treatments. Three *Correspondence: Shannon L. Osborne, USDA-ARS, Northern Grains Insects Research Laboratory, 2923 Medary Ave., Brookings, SD 57006, USA; E-mail: sosborne@ngirl.ars.usda.gov. www.dekker.comThis document is a U.S. government work and is not subject to copyright in the United States.irrigation (whole-plot) treatments included dry land, irrigation based on 0.5, and full evapotranspiration (ET). Sub-plot treatments included five N rates (0, 45, 90, 134, 269 kg N ha À1 ). Multi-spectral imagery consisted of four wavebands; blue (485 nm AE 35 nm), green (550 nm AE 35 nm), red (660 nm AE 30 nm), and near-infrared (NIR) (830 nm AE 70 nm). Imagery was collected on various dates throughout the growing season; biomass sampling was performed within two days of image collection. Grain yield, in-season biomass, and N concentration increased with increasing N rate regardless of sampling date or year. Yield was only affected by irrigation during 1997 due to a significant difference in rainfall between 1997 and 1998, 247 vs. 457 mm, respectively. Regression correlation coefficients for the 1998 values were generally higher compared to 1997 values across years for imagery collected at similar growth stages, possibly due to differences in sensor sensitivity or the increased plant response to applied N. Regression correlation coefficients increased as the growing season progressed. The green waveband and normalized difference greenness vegetation index (GNDVI) had the greatest ability to estimate grain yield in the presence of varying N and/or drought stresses. This study demonstrates the ability of multispectral imagery analysis to estimate grain production in the presence of N and/or drought stresses.