Cannabisin B induces autophagic cell death by inhibiting the AKT/mTOR pathway and S phase cell cycle arrest in HepG2 cells

Abstract

This study investigates the anticancer properties of cannabisin B, purified from hempseed hull, in HepG2 human hepatoblastoma cells. The results indicate that cannabisin B significantly inhibited cell proliferation by inducing autophagic cell death rather than typical apoptosis. Cell viability transiently increased upon the addition of a low concentration of cannabisin B but decreased upon the addition of high concentrations. Cannabisin B-induced changes in cell viability were completely inhibited by pre-treatment with 3-methyladenine (3-MA), indicating that the induction of autophagy by cannabisin B caused cell death. Additionally, cannabisin B induced S phase cell cycle arrest in a dose-dependent manner. Moreover, cannabisin B was found to inhibit survival signaling by blocking the activation of AKT and down-stream targets of the mammalian target of rapamycin (mTOR). These findings suggest that cannabisin B possesses considerable antiproliferative activity and that it may be utilised as a promising chemopreventive agent against hepatoblastoma disease.

Effect of Solvent, Preheating Temperature, and Time on the Ultrasonic Extraction of Phenolic Compounds from Cold‐Pressed Hempseed Cake

“…Pojić et al () detected 0.15 mg g −1 of N ‐ trans ‐caffeoyltyramine in whole cake fractions of hempseed and claimed that the identification of these compounds was solely based on UV spectral characteristics. N ‐ trans ‐Caffeoyltyramine and its derivatives (cannabisin B) were isolated from the hempseed kernel and hull (Chen et al, , ).…”

Hempseed Lignanamides Rich-Fraction: Chemical Investigation and Cytotoxicity towards U-87 Glioblastoma Cells

“…In particular, TOF-MS/MS spectra showed the ions at m/z 269.08 as base peak, whereas the ions at m/z 485.17 (− catechol), 432.14 (likely due to the loss of HNCO + p-hydroxystyrene), and 322.10, which could be favorably formed when the moiety weighing 163.06 Da was lost together with a catechol unit, were detected with their relative high abundance. Cannabisin B, among lignanamides from hempseed, was one of the first to be investigated for its biological behavior, and it was found that it showed a marked antiproliferative action on HepG2 cell [11]. 1468.…”

“…As some of compounds in LnHF were reported to exert neuroprotective and anti-neuroinflammatory effects [20,21], or also to be able to induce dramatic morphological changes and autophagic cell death [11], the effects of the treatment of LnHS hempseed mixture on primary glioblastoma cell lines was investigated. Indeed, a prior analysis of the effects of LnHS was carried out on undifferentiated SH-SY5Y cells.…”

“…Hemp seeds (non-drug type of Cannabis sativa L.) also contain, beyond proteins, and polyunsaturated fatty acids, bioactive lignan derivatives, known as lignanamides [9][10][11][12]. These latter could be found in hemp fruits together with their biosynthetic precursors, namely phenylamides, whose presence is functional in another seed, such as oat seed, which produces avenanthramides (AVAs) with important anti-inflammatory and antiproliferative effects [13].…”

Phyto-Medicine in Gene(s) Targeting Future Direction for Sickle Cell Disease Management

“…Oxidative stress challenges often arise from sources such as radiation, metabolism of xenobiotic, and challenges to the immune system due to abnormal functions [56,75]. From the forgone, SCD are linked to a patho-biological condition with multiple sources of pro-oxidant processes with its attendant consequence and, concomitant chronic and systemic oxidative stress [12,69].…”