Cannabis seed identification by chloroplast and nuclear DNA

Tsai, Li-Chin; Hsieh, Hsing-Mei; Huang, Li-Hung; Wang, Jenn-Che; Linacre, Adrian; Lee, James Chun-I

doi:10.1016/j.forsciint.2005.10.021

Cited by 20 publications

(12 citation statements)

References 6 publications

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“…Extracting opiate from poppy seed is particularly troublesome because poppy seed is an excellent emulsifier. Marijuana ( Cannabis sativa L), another important medicinal plant, can be identified using universal (2) or specific (3) polymerase chain reaction (PCR) primer sets. Knowledge of the genetic structure of the opium poppy would facilitate the control of drug trafficking by enabling detection from tiny fragments (or seeds) of a plant.…”

mentioning

confidence: 99%

An Assessment of the Utility of Universal and Specific Genetic Markers for Opium Poppy Identification

Lee

Hwang

Kim

et al. 2010

Journal of Forensic Sciences

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The proper identification of illicit plants such as Papaver somniferum L (opium poppy) is important for law enforcement agencies. The identification of opium poppy was presently tested using 10 genetic markers that are universal for all plants or specific to a few poppy plants. The genetic distances of universal markers such as nuclear internal transcribed spacer (ITS), 18S rRNA, plastid rbcL, and trnL-trnF intergenic spacer (IGS) of 14 species included in the Papaveraceae and Fumariaceae family were acquired by sequence comparisons. Both the ITS region and trnL-trnF IGS showed high levels of interspecific divergence. Six Papaver genera-specific markers were developed from coding regions involved in morphine biosynthesis. Three markers (TYDC, NCS, and BBE) produced amplicons only in opium poppy, providing a presence/absence test for opium poppy, while three additional markers (CYP80B1, SAT, and COR) were genus specific. These 10 markers might be useful for the forensic DNA analysis of opium poppy.

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confidence: 99%

An Assessment of the Utility of Universal and Specific Genetic Markers for Opium Poppy Identification

Lee

Hwang

Kim

et al. 2010

Journal of Forensic Sciences

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“…C. sativa seeds as a DNA source tend to limit options for efficient DNA extraction techniques, therefore the lack of a quick and accurate species identification method for seeds suspected of being C. sativa has been a problem in law enforcement. Although Sanger sequencing‐based methods have been used in the forensic identification of suspected C. sativa seeds , these DNA sequence‐based techniques are both time consuming and costly.…”

Section: Resultsmentioning

confidence: 99%

Species Identification of Cannabis sativa Using Real‐Time Quantitative PCR (qPCR)^,

Johnson

Premasuthan

Trask

et al. 2013

Journal of Forensic Sciences

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Most narcotics-related cases in the United States involve Cannabis sativa. Material is typically identified based on the cystolithic hairs on the leaves and with chemical tests to identify of the presence of cannabinoids. Suspect seeds are germinated into a viable plant so that morphological and chemical tests can be conducted. Seed germination, however, causes undue analytical delays. DNA analyses that involve the chloroplast and nuclear genomes have been developed for identification of C. sativa materials, but they require several nanograms of template DNA. Using the trnL 3' exon-trnF intragenic spacer regions within the C. sativa chloroplast, we have developed a real-time quantitative PCR assay that is capable of identifying picogram amounts of chloroplast DNA for species determination of suspected C. sativa material. This assay provides forensic science laboratories with a quick and reliable method to identify an unknown sample as C. sativa.

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“…It has been reported that DNAbased assays using PCR or real-time quantitative PCR are able to detect C. sativa, but those assays require long hours for DNA extraction and special equipment, such as a thermal cycler. [14][15][16][17] Loop-mediated isothermal amplification (LAMP) is a nucleotide acid amplification method that features high sensitivity, specificity, and rapidity under isothermal conditions.…”

Section: 7)mentioning

confidence: 99%

Development of Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of <i>Cannabis sativa</i>

Kitamura

Aragane

Nakamura

et al. 2016

Biological & Pharmaceutical Bulletin

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In many parts of the world, the possession and cultivation of Cannabis sativa L. are restricted by law. As chemical or morphological analyses cannot identify the plant in some cases, a simple yet accurate DNA-based method for identifying C. sativa is desired. We have developed a loop-mediated isothermal amplification (LAMP) assay for the rapid identification of C. sativa. By optimizing the conditions for the LAMP reaction that targets a highly conserved region of tetrahydrocannabinolic acid (THCA) synthase gene, C. sativa was identified within 50 min at 60-66°C. The detection limit was the same as or higher than that of conventional PCR. The LAMP assay detected all 21 specimens of C. sativa, showing high specificity. Using a simple protocol, the identification of C. sativa could be accomplished within 90 min from sample treatment to detection without use of special equipment. A rapid, sensitive, highly specific, and convenient method for detecting and identifying C. sativa has been developed and is applicable to forensic investigations and industrial quality control.

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Cannabis seed identification by chloroplast and nuclear DNA

Cited by 20 publications

References 6 publications

An Assessment of the Utility of Universal and Specific Genetic Markers for Opium Poppy Identification

An Assessment of the Utility of Universal and Specific Genetic Markers for Opium Poppy Identification

Species Identification of Cannabis sativa Using Real‐Time Quantitative PCR (qPCR)^,

Development of Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of <i>Cannabis sativa</i>

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Cannabis seed identification by chloroplast and nuclear DNA

Cited by 20 publications

References 6 publications

An Assessment of the Utility of Universal and Specific Genetic Markers for Opium Poppy Identification

An Assessment of the Utility of Universal and Specific Genetic Markers for Opium Poppy Identification

Species Identification of Cannabis sativa Using Real‐Time Quantitative PCR (qPCR),

Development of Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of <i>Cannabis sativa</i>

Contact Info

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Species Identification of Cannabis sativa Using Real‐Time Quantitative PCR (qPCR)^,