Cannabinoids as anti-ROS in aged pancreatic islet cells

Baeeri, Maryam; Rahimifard, Mahban; Daghighi, Seyed Mojtaba; Khan, Fazlullah; Salami, Seyed Alireza; Moini-Nodeh, Shermineh; Haghi‐Aminjan, Hamed; Bayrami, Zahra; Rezaee, Farhad; Abdollahi, Mohammad

doi:10.1016/j.lfs.2020.117969

Cited by 28 publications

(23 citation statements)

References 41 publications

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“…Furthermore, CB 1/2 -independent mechanisms of action for CBD have also been identified, including PPAR-γ [ 13 , 19 , 139 , 140 ], TRPV1 receptor [ 18 ], GPR55 [ 16 ], 5-HT receptors [ 19 , 20 , 144 , 145 ] and μ-/δ-opioid receptors [ 146 ]. Much data suggest that CBD has antioxidant capacity, and CBD has been shown to reduce oxidative metabolism in polymorphonuclear leukocytes [ 168 ] and H 2 O 2 -treated nucleus pulposus cells [ 169 ], and furthemore reduces oxidative stress parameters in aged pancreatic cells [ 170 ]. In addition, CBD has the proclivity to improve cell viability following H 2 O 2 treatment [ 169 ].…”

Section: Antioxidant and Anti-inflammatory Effects Of Cannabinoids: Focus On Cbdmentioning

confidence: 99%

“…In support of this, CBD reduces chemotactic peptide-induced ROS production in polymorphonuclear leukocytes [ 168 ] and furthermore in vivo administration of CBD ameliorates the production of ROS, in addition to lipid peroxides, in myocardial tissue of diabetic mice [ 188 ]. Finally, recent data from Baeeri and colleagues (2020) indicate that CBD attenuates age-related increases in ROS production in pancreatic islets [ 170 ], overall indicating the proclivity of CBD to exert free radical scavenging capacity in response to a multitude of stimuli.…”

Section: Antioxidant Mechanisms Of Action Of Cbdmentioning

confidence: 99%

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Cannabidiol modulation of oxidative stress and signalling

et al. 2021

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Cannabidiol (CBD), one of the primary non-euphoric components in the Cannabis sativa L. plant, has undergone clinical development over the last number of years as a therapeutic for patients with Lennox-Gastaut syndrome and Dravet syndromes. This phytocannabinoid demonstrates functional and pharmacological diversity, and research data indicate that CBD is a comparable antioxidant to common antioxidants. This review gathers the latest knowledge regarding the impact of CBD on oxidative signalling, with focus on the proclivity of CBD to regulate antioxidants and control the production of reactive oxygen species. CBD is considered an attractive therapeutic agent for neuroimmune disorders, and a body of literature indicates that CBD can regulate redox function at multiple levels, with a range of downstream effects on cells and tissues. However, pro-oxidant capacity of CBD has also been reported, and hence caution must be applied when considering CBD from a therapeutic standpoint. Such pro- and antioxidant functions of CBD may be cell- and model-dependent, and may also be influenced by CBD dose, the duration of CBD treatment and the underlying pathology.

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Section: Antioxidant and Anti-inflammatory Effects Of Cannabinoids: Focus On Cbdmentioning

confidence: 99%

Section: Antioxidant Mechanisms Of Action Of Cbdmentioning

confidence: 99%

Cannabidiol modulation of oxidative stress and signalling

et al. 2021

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“…It is believed that oxidative stress can cause DNA damage, followed by DNA Damage Response (DDR). DDR consists of complex cascades, such as cell cycle arrest, that finally may result in cellular senescence [45,46]. One of the main things in DDR is P38 activation by MAPKs that results in P16 activation.…”

Section: Discussionmentioning

confidence: 99%

Vinpocetine Effect on the Juncture of Diabetes and Aging: An in-vitro study

et al. 2021

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Background The rapid-growing population of diabetic patients and the elderly are among the direst challenges that the science of medicine is facing today. Targeting these two challenges can shed light on new means to control and ideally reverse this trend. In this experiment, Vinpocetine’s effect on aged pancreatic beta-cell functions in correlation with oxidative stress was studied. Methods Islet cells were isolated from the pancreas of aged rats and exposed to Vinpocetine, dissolved in acetone and RPMI, for 48 h. Then, senescence-associated molecular parameters, including P16 and P38 gene expressions and β-galactosidase activity, were investigated along with diabetic and inflammation markers. Results Experimental results showed that Vinpocetine could significantly increase aged islets insulin secretion and also make a meaningful reduction in oxidative stress markers. This drug can also decrease expression levels of P16 and P38, the primary genes responsible for the aging pathway. TNF-α, IL-6, and NF-κB expressions were also reduced noticeably after treatment with Vinpocetine. Conclusion The current study showed that Vinpocetine, a derivative of the secondary plant metabolite called Vincamine, could break this vicious cycle of oxidative stress and aging by reducing oxidative stress and inflammation, thus inhibiting cellular aging.

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“…The supernatant was collected then the protein concentration was measured using a BCA Protein Assay Kit. Next, ELISA was used to detect effects of PNO treatment on SA-β-Gal activity in replicative senescent NIH-3T3 cells according to the manufacturer’s instructions ( Baeeri et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

A Novel Biochemical Study of Anti-Dermal Fibroblast Replicative Senescence Potential of Panax Notoginseng Oligosaccharides

Zhai

Liu

et al. 2021

Front. Pharmacol.

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Dermal fibroblast replicative senescence that often occurs in aging skin is characterized by loss of cell proliferative capacity, cell cycle arrest, decreased cell elongation, and decreased synthesis of dermal extracellular matrix (ECM) components. Although Panax notoginseng is known for its effectiveness in alleviating many age-related degenerative diseases, few studies have evaluated P. notoginseng components for efficacy or mechanisms of action in delaying cell replicative senescence. In this study, P. notoginseng oligosaccharides (PNO) were isolated using a stepwise purification procedure involving water extraction and alcohol precipitation followed by DEAE Sepharose Fast Flow column chromatography, preparative high performance liquid chromatography, and size-exclusion chromatography. Monosaccharides detected in PNO constituents included mannose, galactose, and sorbitose in relative molar proportions of 14.2:12.3:1, respectively, aligning with PNO absorption spectrum results resembling typical known spectra for sugars. In vitro, PNO treatment of replicative senescent NIH-3T3 fibroblasts significantly promoted cell vitality, inhibited SA-β-galactosidase (SA-β-Gal) activity, and reduced p16 and p21 protein-level expression. Moreover, PNO treatment of senescent fibroblasts led to a lower proportion of G1 phase cells and higher proportion of S phase cells, while also inducing aging NIH-3T3 cells to migrate and synthesize collagen-I (CoL-I). Mechanistically, PNO treatment up-regulated expression of proliferating cell nuclear antigen (PCNA), cyclin E, cyclin D1, and cyclin-dependent kinase 4 (CDK4) proteins and promoted phosphorylation of MEK, p38, and ERK1/2 to trigger cell cycle progression. Additionally, PNO treatment also up-regulated protein-level expression of TGF-β1 and levels of p-Smad2/3, p-FAK, and p-Pax to trigger CoL-I synthesis and cell migration. Taken together, these findings demonstrate that oligosaccharides purified from P. notoginseng could reverse fibroblast replicative senescence by promoting fibroblast cell proliferation, migration, and CoL-I production.

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Cannabinoids as anti-ROS in aged pancreatic islet cells

Cited by 28 publications

References 41 publications

Cannabidiol modulation of oxidative stress and signalling

Cannabidiol modulation of oxidative stress and signalling

Vinpocetine Effect on the Juncture of Diabetes and Aging: An in-vitro study

A Novel Biochemical Study of Anti-Dermal Fibroblast Replicative Senescence Potential of Panax Notoginseng Oligosaccharides

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