Cannabidiol-loaded microparticles embedded in a porous hydrogel matrix for biomedical applications

Abstract

In this study, poly (lactic-co-glycolic acid) (PLGA) microparticles loaded with cannabidiol (CBD) were synthesized (PLGA@CBD microparticles) and embedded up to 10 wt% in a chondroitin sulfate/polyvinyl alcohol hydrogel matrix. In vitro chemical, physical, and biological assays were carried out to validate the potential use of the modified hydrogels as biomaterials. The microparticles had spherical morphology and a narrow range of size distribution. CBD encapsulation efficiency was around 52%, loading was approximately 50%. Microparticle addition to the hydrogels caused minor changes in their morphology, FTIR and thermal analyses confirmed these changes. Swelling degree and total porosity were reduced in the presence of microparticles, but similar hydrophilic and degradation in phosphate buffer solution behaviors were observed by all hydrogels. Rupture force and maximum strain at rupture were higher in the modified hydrogels, whereas modulus of elasticity was similar across all materials. Viability of primary human dental pulp cells up to 21 days was generally not influenced by the addition of PLGA@CBD microparticles. The control hydrogel showed no antimicrobial activity against Staphylococcus aureus, whereas hydrogels with 5% and 10% PLGA@CBD microparticles showed inhibition zones. In conclusion, the PLGA@CBD microparticles were fabricated and successfully embedded in a hydrogel matrix. Despite the hydrophobic nature of CBD, the physicochemical and morphological properties were generally similar for the hydrogels with and without the CBD-loaded microparticles. The data reported in this study suggested that this original biomaterial loaded with CBD oil has characteristics that could enable it to be used as a scaffold for tissue/cellular regeneration. Graphical Abstract

How Biodegradable Polymers Can be Effective Drug Delivery Systems for Cannabinoids? Prospectives and Challenges

“…Higher concentration of PLGA-CBD microparticles in hydrogels exhibited increased antimicrobial activity. [ 246 ] Micelles CBD Cinnamyl modified polyglycolide/PCL PG 50 - b -PPO 4 - b -[P(CyCL) 4 - co -(CL) 40 ]- b- PPO 4 - b- PG 50 51.0 ± 1.8 nm ζ = −6.61 ± 3.10 mV EE = 95.0% In PBS pH 7.4: about 35% of the drug was released within 24h. HL-60 and HUT-78 Against HL-60: IC 50 = 3.00 µg/mL Against HUT-78: IC 50 = 8.30 µg/mL - - - - CBD was loaded during micelle formation.…”

Antimicrobial, Probiotic, and Immunomodulatory Potential of Cannabis sativa Extract and Delivery Systems

“…Developing delivery systems is essential for enhancing their therapeutic potential. So far, various delivery systems, including nanostructured lipid carriers for encapsulating cannabinoids (URB597, AM251, and rimonabant), zeolite-thymol composites, microencapsulated cannabidiol in liposomes, and poly(lactic-co-glycolic acid) microparticles loaded with cannabidiol were prepared [67][68][69][70]. These systems have impacted stability and antimicrobial potential and offer implementation in food and personal care applications, such as sanitary materials or biomedical applications.…”