Canine zona pellucida glycoprotein-3: Up-scaled production, immunization strategy and its outcome on fertility

Shrestha, Anmol Purna; Srichandan, Sudeepa; Minhas, Vidisha; Panda, Amulya K.; Gupta, Seema

doi:10.1016/j.vaccine.2014.11.003

Cited by 14 publications

(12 citation statements)

References 33 publications

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“…Similarly, their AMH values were intermediate between pZP and control groups between one and 6 months post-V2. This finding encourages further research aimed at improved efficacy of reZP in mares, such as the administration of additional booster vaccinations [28] or alternative formulations such as the incorporation of liposomes [7].…”

Section: Discussionmentioning

confidence: 62%

Serum anti-Müllerian hormone dynamics in mares following immunocontraception with anti-zona pellucida or -GnRH vaccines

et al. 2018

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Circulating anti-Müllerian hormone concentration (AMH) is positively correlated to the number of small growing follicles in the mare and may reflect ovarian function. Dynamics of AMH during immunocontraception have not previously been investigated. This study aimed to compare serum AMH in mares following treatment with native porcine zona pellucida (pZP), recombinant pZP3 and pZP4 (reZP) or gonadotrophin releasing hormone (GnRH) vaccines, and saline-treated controls. Stored sera collected during two previous studies examining ovarian activity in mares during zona pellucida (ZP) or GnRH immunocontraception were analysed for serum AMH. Data were compared among treatment groups using mixed-effects linear regression and one-way ANOVA with post hoc testing. Correlations between AMH and previously reported clinical variables were estimated using Spearman's rho. Mares immunized against GnRH showed variable but detectable AMH throughout successive breeding and non-breeding seasons that were not significantly different to unvaccinated control mares. Mares treated with pZP demonstrated marked, reversible suppression of AMH. Mares immunized using reZP showed an intermediate effect. In the ZP study, AMH was positively correlated to serum progesterone concentrations, mean ovarian volumes and antral follicle counts, whereas no correlations between AMH and serum progesterone concentrations, mean ovarian volumes, or the presence of one or more follicles ≥20 mm in diameter were detected in the GnRH study. In conclusion, marked suppression of AMH during pZP immunocontraception, but not during GnRH immunocontraception, suggested enhanced suppression of ovarian follicular development and, or follicular function during pZP immunocontraception. Serum AMH concentrations may provide a novel tool for the assessment of ovarian function during ZP-based immunocontraception.

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Section: Discussionmentioning

confidence: 62%

Serum anti-Müllerian hormone dynamics in mares following immunocontraception with anti-zona pellucida or -GnRH vaccines

et al. 2018

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“…Recently, it was shown that 2 boosters of recombinant dog ZP3, instead of one, showed better contraceptive efficacy in female mice [31]. A third possibility would be to increase the number of booster vaccinations.…”

Section: Discussionmentioning

confidence: 99%

“…A third possibility would be to increase the number of booster vaccinations. Recently, it was shown that 2 boosters of recombinant dog ZP3, instead of one, showed better contraceptive efficacy in female mice [31].…”

Section: Discussionmentioning

confidence: 99%

Ovarian function and pregnancy outcome in pony mares following immunocontraception with native and recombinant porcine zona pellucida vaccines

Joone

Bertschinger

Gupta

et al. 2016

Equine Veterinary Journal

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“…Indeed, a correlation between antibody titer and infertility was observed in this group of mice compared to those immunized with TT‐KK‐Sp17 N , whose antisera was effective at blocking in vitro fertilization but not in vivo fertility (Tables and ). A previous study demonstrating that actively immunized female mice with TT‐KK‐ZP3 also identified a correlation between antibody titer and infertility (Shrestha et al, )—although the predictive power of antibody titer versus contraception is still unclear (Goldberg et al, ; O'Hern et al, ). Interestingly, 80% of the Sp17 C ‐GnRH 2 ‐immunized female mice failed to conceive, compared to 50% of mice immunized with TT‐KK‐Sp17 C , suggesting that incorporation of GnRH increased the contraceptive efficacy of Sp17 C .…”

Section: Discussionmentioning

confidence: 99%

Novel Sperm and Gonadotropin‐releasing Hormone‐based Recombinant Fusion Protein: Achievement of 100% Contraceptive Efficacy by Co‐immunization of Male and Female Mice

Minhas

Shrestha

Wadhwa

et al. 2016

Molecular Reproduction Devel

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Improvements in long-term female contraception can be achieved by vaccinating with sperm-derived proteins. Here, recombinant proteins comprising either (i) N- (amino acid residues 1-80) or C- (amino acid residues 76-126) terminal fragments of mouse sperm protein 17 (Sp17) fused to the promiscuous T non-B cell epitope of tetanus toxoid (TT), amino acid residues 830-844 followed by di-lysine linker (KK) (TT-KK-Sp17 or TT-KK-Sp17 , respectively) or (ii) mouse equatorin (amino acid residues 21-185) fused to the T non-B cell epitope of bovine RNase (amino acid residues 94-104) were expressed in Escherichia coli. Immunization of female FVB/J mice, using alum as an adjuvant, led to the generation of high antibody titers against the above proteins. Antibodies against both N- and C-terminal fragments of Sp17 reacted with the entire capacitated mouse spermatozoa, whereas those against equatorin reacted exclusively with the equatorial region. Despite the reactivity of all immune sera, only sera from mice immunized with TT-KK-Sp17 and TT-KK-Sp17 significantly reduced mouse in vitro fertilization. Mating studies of the immunized females with un-immunized male mice revealed the highest infertility in the TT-KK-Sp17 -immunized group. In an attempt to further boost the immune response, the C-terminal fragment of Sp17 was expressed as fusion protein with a tandem repeat of gonadotropin-releasing hormone (GnRH) (Sp17 -GnRH ). Immunization of both male and female mice with Sp17 -GnRH led to higher contraceptive efficacy compared to mice immunized with TT-KK-Sp17 . Interestingly, mating studies wherein partners were both immunized with Sp17 -GnRH showed a complete failure of female mice to conceive. Thus, immunization of both males and females with Sp17 -GnRH has the potential to increase contraceptive efficacy. Mol. Reprod. Dev. 83: 1048-1059, 2016. © 2016 Wiley Periodicals, Inc.

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Canine zona pellucida glycoprotein-3: Up-scaled production, immunization strategy and its outcome on fertility

Cited by 14 publications

References 33 publications

Serum anti-Müllerian hormone dynamics in mares following immunocontraception with anti-zona pellucida or -GnRH vaccines

Serum anti-Müllerian hormone dynamics in mares following immunocontraception with anti-zona pellucida or -GnRH vaccines

Ovarian function and pregnancy outcome in pony mares following immunocontraception with native and recombinant porcine zona pellucida vaccines

Novel Sperm and Gonadotropin‐releasing Hormone‐based Recombinant Fusion Protein: Achievement of 100% Contraceptive Efficacy by Co‐immunization of Male and Female Mice

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