Canine vector-borne pathogens from dogs and ticks from Tamil Nadu, India

Manoj, Ranju Ravindran Santhakumari; Iatta, Roberta; Latrofa, Maria Stefanía; Capozzi, Loredana; Raman, Muthusamy; Colella, Vito; Otranto, Domenico

doi:10.1016/j.actatropica.2019.105308

Cited by 33 publications

(23 citation statements)

References 72 publications

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“…B. vogeli has also been identified in nymphal and adult ticks, indicating a significant transstadial transmission in the lifecycle of this Babesia species and the possible existence of parasite competition for the tick vector, Rhipicephalus sanguineus [78,79]. Another PCR assay with the V4 variable region of 18S rRNA gene as a target, combined with Reverse Line Blot (RLB) hybridization and DNA sequencing of the amplified products [80], was used to identify B. vogeli in R. sanguineus ticks from Tunisia [81], R. sanguineus and R. turanicus from Israel [82], R. sanguineus and R. haemaphysaloides from India [83], and to define the local vector for canine babesiosis in Taiwan [84] (Table 2). In Taiwan, primers used for PCR amplification were RLB-F2/RLB-R2, and commercial oligonucleotides probes with B. vogeli and B. gibsoni as targets were used for RLB hybridization.…”

Section: Molecular Toolsmentioning

confidence: 99%

Challenges in Tick-Borne Pathogen Detection: The Case for Babesia spp. Identification in the Tick Vector

et al. 2021

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The causative agents of Babesiosis are intraerythrocytic protozoa of the genus Babesia. Babesia parasites are present around the world, affecting several mammals including humans, pets and livestock, hence its medical and veterinary relevance. Babesia spp. detection in its invertebrate host is a main point in understanding the biology of the parasite to acquire more knowledge on the host–Babesia–vector interactions, as increasing knowledge of the Babesia lifecycle and babesiosis epidemiology can help prevent babesiosis outbreaks in susceptible mammals. The aim of the present review is to highlight the newest findings in this field, based on a bibliographic compilation of research studies recently carried out for the detection of the main Babesia species found in tick vectors affecting mammalian hosts, including the different tick stages such as adult ticks, larvae, nymphs and eggs, as well as the detection method implemented: microscopic tools for parasite identification and molecular tools for parasite DNA detection by conventional PCR, nested-PCR, PCR-RFLP, PCR-RLB hybridization, real time-PCR, LAMP and RAP assays. Although molecular identification of Babesia parasites has been achieved in several tick species and tissue samples, it is still necessary to carry out transmission experiments through biological models to confirm the vectorial capacity of various tick species.

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Section: Molecular Toolsmentioning

confidence: 99%

Challenges in Tick-Borne Pathogen Detection: The Case for Babesia spp. Identification in the Tick Vector

et al. 2021

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“…Contrary the prognosis remains good for B. vogeli infections, the large form of canine Babesia infecting dogs in India (Solano-Gallego et al, 2016). However, Laha et al (2014) and Manoj et al (2020) reported higher prevalence of B. canis/B. vogeli infection (54.05% and 10.00%, respectively) in dogs in Assam and Tamil Nadu, respectively, compared to that of B. gibsoni infection (48.64% and 0.4%, respectively) using conventional PCR.…”

Section: Resultsmentioning

confidence: 95%

“…Though canine babesiosis has been reported from different parts of India based on microscopic examination and molecular assays (Shaw et al, 2001;Senthil Kumar et al, 2009;Abd Rani et al, 2011;Laha et al, 2014;Manoj et al, 2020), there is paucity of information regarding the prevalence and molecular confirmation of Babesia species in dogs in Andhra Pradesh (AP), India. Keeping in view of the above information, the present study was aimed to detect Babesia species in naturally infected dogs in Andhra Pradesh, India by microscopy and conventional PCR and to determine possible factors associated with canine babesiosis in the study area.…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection and Risk Factor Analysis of Babesia gibsoni and Babesia vogeli in Naturally Infected Dogs in Andhra Pradesh, India

Kopparthi

Sreedevi

Vukka

et al. 2021

IJAR

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Background: Babesiosis in dogs is endemic in tropical countries like India because of presence of tick vectors as well as reservoir stray dog population round the year. There is paucity of information regarding the prevalence and molecular confirmation of Babesia species in dogs in Andhra Pradesh. The present study was aimed to detect Babesia species in naturally infected dogs in Andhra Pradesh.Methods: During the one-year study i.e. from June 2019 to July 2020, blood samples were collected from 442 tick infected dogs showing clinical signs suggestive of babesiosis to determine the prevalence of Babesia species by microscopy and molecular technique. Factors associated with prevalence of babesiosis were analyzed by logistic regression models.Result: Microscopic examination of stained blood smears revealed 11.8% prevalence of Babesia spp. infection in dogs in the study area, while 28.3% of dogs were found to be positive for Babesia spp. in the first Babesia genus-specific PCR. Further analysis of positive samples with species-specific PCR, Babesia gibsoni and B. vogeli were detected 19.9% and 8.6% of dogs, respectively. Risk factor analysis by binary logistic regression model revealed that the living condition of the dogs, gender, age, medium and tick infestation as risk factors for B. gibsoni and B. vogeli infection.

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“…4 Furthermore, Candidatus D. hongkongensis has been identified in domestic and wild canine populations in South India. 8,16,17 Likewise, Dirofilaria sp. closely related with Candidatus D. hongkongensis has been isolated from dogs in Thailand.…”

Section: Discussionmentioning

confidence: 99%

Case Report: An Eyelid Nodule Caused by Candidatus Dirofilaria hongkongensis Diagnosed by Mitochondrial 12S rRNA Sequence

Tirakunwichcha

Sansopha

Putaporntip

et al. 2021

The American Journal of Tropical Medicine and Hygiene

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A 59-year-old female living in Rayong Province, eastern Thailand, presented with painless, right upper eyelid nodule for 3 months. Upon removal of the eyelid mass, a well-circumscribed, firm globular mass with diameter about 1 cm was found. Histopathological examination revealed an immature female dirofilarial worm reminiscent of Dirofilaria repens, characterized by prominent sharp longitudinal ridges at external surface of the cuticle. Analysis of the mitochondrial 12S rRNA sequence showed that the worm belongs to Candidatus Dirofilaria hongkongensis. It is likely that some infections previously reported as D. repens based on histological examination may have actually been due to Candidatus D. hongkongensis.

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Canine vector-borne pathogens from dogs and ticks from Tamil Nadu, India

Cited by 33 publications

References 72 publications

Challenges in Tick-Borne Pathogen Detection: The Case for Babesia spp. Identification in the Tick Vector

Challenges in Tick-Borne Pathogen Detection: The Case for Babesia spp. Identification in the Tick Vector

Molecular Detection and Risk Factor Analysis of Babesia gibsoni and Babesia vogeli in Naturally Infected Dogs in Andhra Pradesh, India

Case Report: An Eyelid Nodule Caused by Candidatus Dirofilaria hongkongensis Diagnosed by Mitochondrial 12S rRNA Sequence

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