Canine scent detection of sinonasal-inverted papilloma in blood plasma and nasal secretions

Mallikarjun, Amritha; Collins, Amanda; Verta, Alec; Hanadari-Levy, Amit; Chaskes, Mark B.; Rosen, Marc; Nyquist, Gurston; Toskala, Elina; Rabinowitz, Mindy; Otto, Cynthia M.

doi:10.1016/j.jveb.2023.02.008

Cited by 3 publications

(3 citation statements)

References 34 publications

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“…In this case, dogs were trained on fecal matter from CWD-positive deer, but they saw training aids incubated with CWDpositive fecal matter during the test. Previous studies have suggested that changes in the target medium can reduce the rate of trained final alerts for the target odor; however, dogs still show a change in behavior for the new target medium, suggesting that they still recognize the odor in a different medium and can differentiate it from the control and negative samples [34].…”

Section: Change In Behavior Analysesmentioning

confidence: 92%

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Assessing Different Chronic Wasting Disease Training Aids for Use with Detection Dogs

Mallikarjun,

Charendoff,

Moore

et al. 2024

Animals

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Chronic wasting disease (CWD) is a highly infectious, fatal prion disease that affects cervid species. One promising method for CWD surveillance is the use of detection dog–handler teams wherein dogs are trained on the volatile organic compound signature of CWD fecal matter. However, using fecal matter from CWD-positive deer poses a biohazard risk; CWD prions can bind to soil particles and remain infectious in contaminated areas for extended periods of time, and it is very difficult to decontaminate the affected areas. One solution is to use noninfectious training aids that can replicate the odor of fecal matter from CWD-positive and CWD-negative deer and are safe to use in the environment. Trained CWD detection dogs’ sensitivity and specificity for different training aid materials (cotton, GetXent tubes, and polydimethylsiloxane, or PDMS) incubated with fecal matter from CWD-positive and CWD-negative deer at two different temperatures (21 °C and 37 °C) for three different lengths of time (6 h, 24 h, and 48 h) were evaluated. Cotton incubated at 21 °C for 24 h was identified as the best aid for CWD based on the dogs’ performance and practical needs for training aid creation. Implications for CWD detection training and for training aid selection in general are discussed.

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Section: Change In Behavior Analysesmentioning

confidence: 92%

“…Given that dogs spent, on average, 0.65 s (st. dev. = 0.30 s) checking control odors in previous studies [12,34], if the dogs spent 1 s or more at any odor source, this activity was considered a change in behavior.…”

Section: Change In Behavior Analysesmentioning

confidence: 99%

Assessing Different Chronic Wasting Disease Training Aids for Use with Detection Dogs

Mallikarjun,

Charendoff,

Moore

et al. 2024

Animals

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“…Another study demonstrated canines' ability to detect sinonasal-inverted papilloma (SNIP) after initial training with UDC. Four canines were trained to detect UDC searching an eight-port circular scent wheel and then were trained to detect SNIP within blood plasma; the canines were also able to generalize the SNIP odor and detected SNIP in nasal secretions without additional training ( 58 ). UDC has also been used to assess impacts on canine olfactory capabilities including demonstrating that canine ability to detect UDC was not affected by intravenous fentanyl sedation followed by naloxone reversal.…”

Section: Role In Research Development Test and Evaluation (Rdtande)mentioning

confidence: 99%

Calibrating canines—a universal detector calibrant for detection dogs

Maughan,

Gadberry,

Sharpes

et al. 2024

Front. Allergy

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Since the advent of the Universal Detector Calibrant (UDC) by scientists at Florida International University in 2013, this tool has gone largely unrecognized and under-utilized by canine scent detection practitioners. The UDC is a chemical that enables reliability testing of biological and instrumental detectors. Training a biological detector, such as a scent detection canine, to respond to a safe, non-target, and uncommon compound has significant advantages. For example, if used prior to a search, the UDC provides the handler with the ability to confirm the detection dog is ready to work without placing target odor on site (i.e., a positive control), thereby increasing handler confidence in their canine and providing documentation of credibility that can withstand legal scrutiny. This review describes the UDC, summarizes its role in canine detection science, and addresses applications for UDC within scent detection canine development, training, and testing.

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Dogs can detect an odor profile associated with Staphylococcus aureus biofilms in cultures and biological samples

Ramos,

Chang,

Wilson

et al. 2024

Front. Allergy

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IntroductionThe study investigated the utilization of odor detection dogs to identify the odor profile of Staphylococcus aureus (S. aureus) biofilms in pure in vitro samples and in in vivo biosamples from animals and humans with S. aureus periprosthetic joint infection (PJI). Biofilms form when bacterial communities aggregate on orthopedic implants leading to recalcitrant infections that are difficult to treat. Identifying PJI biofilm infections is challenging, and traditional microbiological cultures may yield negative results even in the presence of clinical signs.MethodsDogs were trained on pure in vitro S. aureus biofilms and tested on lacrimal fluid samples from an in vivo animal model (rabbits) and human patients with confirmed S. aureus PJI.ResultsThe results demonstrated that dogs achieved a high degree of sensitivity and specificity in detecting the odor profile associated with S. aureus biofilms in rabbit samples. Preliminary results suggest that dogs can recognize S. aureus volatile organic compounds (VOCs) in human lacrimal fluid samples.DiscussionTraining odor detection dogs on in vitro S. aureus, may provide an alternative to obtaining clinical samples for training and mitigates biosecurity hazards. The findings hold promise for culture-independent diagnostics, enabling early disease detection, and improved antimicrobial stewardship. In conclusion, this research demonstrates that dogs trained on in vitro S. aureus samples can identify the consistent VOC profile of PJI S. aureus biofilm infections. The study opens avenues for further investigations into a retained VOC profile of S. aureus biofilm infection. These advancements could revolutionize infectious disease diagnosis and treatment, leading to better patient outcomes and addressing the global challenge of antimicrobial resistance.

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Canine scent detection of sinonasal-inverted papilloma in blood plasma and nasal secretions

Cited by 3 publications

References 34 publications

Assessing Different Chronic Wasting Disease Training Aids for Use with Detection Dogs

Assessing Different Chronic Wasting Disease Training Aids for Use with Detection Dogs

Calibrating canines—a universal detector calibrant for detection dogs

Dogs can detect an odor profile associated with Staphylococcus aureus biofilms in cultures and biological samples

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