Canine rotavirus C strain detected in Hungary shows marked genotype diversity

Abstract

Species C rotaviruses (RVC) have been identified in humans and animals, including pigs, cows and ferrets. In dogs, RVC strains have been reported anecdotally on the basis of visualization of rotavirus-like virions by electron microscopy combined with specific electrophoretic migration patterns of the genomic RNA segments. However, no further molecular characterization of these viruses was performed. Here, we report the detection of a canine RVC in the stool of a dog with enteritis. Analysis of the complete viral genome uncovered distinctive genetic features of the identified RVC strain. The genes encoding VP7, VP4 and VP6 were distantly related to those of other RVC strains and were putatively classified as G10, P8 and I8, respectively. The new strain was named RVC/Dog-wt/HUN/KE174/2012/G10P [8]. Phylogenetic analyses revealed that canine RVC was most closely related to bovine RVC strains with the exception of the NSP4 gene, which clustered together with porcine RVC strains. These findings provide further evidence for the genetic diversity of RVC strains.

Candidate new rotavirus species in Schreiber's bats, Serbia

“…Recent years have witnessed considerable sequence data accumulation in public data bases pointing out the enormous genetic diversity within the Rotavirus genus. Viral metagenomics largely contributed to our understanding of this genetic diversity (Asano et al, 2016;He et al, 2013;Kluge et al, 2016;Li et al, 2011;Marton et al, 2015;Mihalov-Kovács et al, 2015;Theuns et al, 2016;Xia et al, 2014). Until the early 2000s RVA to RVG were considered as the only extant RV species (Estes and Greenberg, 2013;Matthijnssens et al, 2012).…”

Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene

“…After its discovery in swine population (Saif et al 1980), RVC has been detected in human, ferret, mink, cattle and dog from many countries (Torres-Medina 1987;Tsunemitsu et al 1991;Saif and Jiang 1994;Otto et al 1999;Mawatari et al 2004;Wise et al 2009;Marton et al 2015). It is now a well-established etiological agent of diarrheal outbreaks for neonatal and adult gastroenteritis (Araujo et al 2011;Kumazaki and Usuku 2014).…”

Unbiased whole-genome deep sequencing of human and porcine stool samples reveals circulation of multiple groups of rotaviruses and a putative zoonotic infection

“…This is probably because the majority of RV investigations have been focused on RVA given its clinical and public health relevance and the large genetic distance among these groups of rotaviruses as compared with RVA, which would likely be missed by commonly used diagnostic assays. Recently, there have been increasing numbers of reports on RV groups B, C, and H in animals (Marthaler et al 2012; Amimo, Vlasova, and Saif 2013; Marthaler et al 2013; Lahon et al 2014; Marthaler et al 2014; Molinari, Alfieri, and Alfieri 2014; Molinari et al 2014; Marton et al 2015; Otto et al 2015) and humans (Alam et al 2013; Lahon et al 2013; Zhirakovskaia et al 2016), which possibly reflect improved molecular methods to detect these viruses rather than an actual increase in their prevalence. In Vietnam, the frequencies and relative role in human and animal disease of RVB, RVC, and RVH viruses are not yet known.…”