Canine MHC‐class II antigens on B and T lymphocytes¹

Abstract

The Nethe-lands MHC-class I1 antigens on canine lymphoid cells were detected with monoclonal antibodies (Mabs). On the basis of reactivity with resting or activated T lymphocytes two groups of Mabs were reported earlier. Non-activated T lymphocytes expressed MHC-class I1 antigens recognized by the first group whereas the second group recognized only MHC-class I1 antigens on activated T lymphocytes. In this study we analysed the reactivity pattern of both groups of Mabs with purified canine B lymphocytes. One-and two-colour immunoflowcytometric analysis, immunoprecipitation, immunohistology and MLC inhibition were performed. The Mabs which only reacted with activated T lymphocytes appeared to stain also purified sIg' lymph node cells. Two-colour fluorescence and immunohistology confirmed the reactivity of these Mabs with B lymphocytes. Immunoprecipitation showed the bimolecular structure of these antigens. It was concluded that two subsets of MHCclass I1 antigens can be detected on canine lymphoid cells. One subset showed an aberrant distribution being expressed on both nonactivated and activated T and on B lymphocytes. The second subset of MHC-class I1 antigens could only be detected on B lymphocytes and activated T lymphocytes, a distribution pattern similar to that found in most other species, for instance man.

Comparative Analysis of mRNA Expression of Surface Antigens between Histiocytic and Nonhistiocytic Sarcoma in Dogs

“…This value was superior to the diagnostic reliability previously reported for other methodologies for diagnosing HS in dogs . MHC class IIα was expressed in particular leukocytes, including lymphoid cells, DCs, macrophages, and other antigen‐presenting cells . The present results showed that MHC class IIα mRNA expression was comparatively higher in HS cells than in other cells originating from leukocytes, including inflammatory and lymphomatous cells.…”

“…MHC class IIα, CD11b, and CD11c are expressed to some extent in tissues and organs of normal dogs, as well as on antigen‐presenting cells such as DCs and macrophages . We experimentally determined the mRNA expression levels of these SAs in several organs of normal dogs, including the bladder, bone marrow, gastrointestinal tract, heart, kidney, liver, lung, lymph nodes, muscle, pancreas, spleen, and subcutaneous tissues.…”

“…The cDNA samples were subjected to activation at 95°C for 3 minutes, followed by 40 cycles of denaturation at 95°C for 20 seconds, and annealing/extension at 60°C for 20 seconds. The selected SAs are expressed relatively at low levels in leukocytes, including as DCs and macrophages, and in normal tissues and organs in dogs . Therefore, all SA expression levels were calibrated using organs or tissues of healthy dogs matching the location of the HS lesion .…”

Joint Report of the Fifth International Workshop on Lymphocyte Alloantigens of the Horse, Baton Rouge, Louisiana, 31 October‐1 November 1987

“…If class I1 antigens are normally expressed on a majority of equine peripheral blood lymphocytes, as is the case in dogs (Doveren et al 1986), this would make serological detection of class I1 antigens possible on unseparated PBL. At the same time, these results may reflect a variability in the expression of class I1 antigens on peripheral blood cells in the horse causing these apparently conflicting reports and also reflected in the technical difficulties encountered by laboratories during the workshop.…”

Role of MHC class II expressing CD4+ T cells in proteolipid protein91–110‐induced EAE in HLA‐DR3 transgenic mice

“…In cattle and horses, activated T cells were observed to express class II on their cell surface [27,28]. MHC class II canine expression has been found on both naïve as well as activated T cells [29]. Activated rat T cells have been reported to express MHC class II molecules [30,31].…”