Canine Mast Cell Tumors: A Comparison of Staining Techniques

Simões, J.; Schoning, Polly

doi:10.1177/104063879400600410

Cited by 40 publications

(36 citation statements)

References 26 publications

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“…22 It has been assumed that this decrease in the staining pattern is due to the lack of production or storage of cellular components that react with the stain. 22 We hypothesized that well-differentiated MCTs have a stronger cytoplasmic expression of tryptase than poorly differentiated MCTs and that such a difference in expression could be used to predict the biologic behavior of canine cutaneous MCTs. To test these hypotheses a retroprospective study was conducted.…”

Section: Introductionmentioning

confidence: 99%

The Use of KIT and Tryptase Expression Patterns as Prognostic Tools for Canine Cutaneous Mast Cell Tumors

et al. 2004

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Abstract. Cutaneous mast cell tumors (MCTs) are one of the most common tumors in dogs. Currently, prognostic and therapeutic determinations for MCTs are primarily based on the histologic grade of the tumor, but a vast majority of MCTs are of an intermediate grade, and the prognostic relevance is highly questioned. A more detailed prognostic evaluation, especially of grade 2 canine MCTs, is greatly needed. To evaluate the prognostic significance of KIT and tryptase expression patterns in canine cutaneous MCTs, we studied 100 cutaneous MCTs from 100 dogs that had been treated with surgery only. The total survival and disease-free survival time and the time to local or distant recurrence of MCTs were recorded for all dogs. Using immunohistochemistry, 98 of these MCTs were stained with anti-KIT and antitryptase antibodies. Three KIT-and three tryptase-staining patterns were identified. The KIT-staining patterns were identified as 1) membrane-associated staining, 2) focal to stippled cytoplasmic staining with decreased membrane-associated staining, and 3) diffuse cytoplasmic staining. The tryptase-staining patterns were identified as 1) diffuse cytoplasmic staining, 2) stippled cytoplasmic staining, and 3) little to no cytoplasmic staining. Based on univariate and multivariate survival analysis, increased cytoplasmic KIT staining was significantly associated with an increased rate of local recurrence and a decreased survival rate. The tryptase-staining patterns were not significantly associated with any survival parameter. On the basis of these results, we propose a new prognostic classification of canine cutaneous MCTs, according to their KIT-staining pattern, that can be used for the routine prognostic evaluation of canine cutaneous MCTs.

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Section: Introductionmentioning

confidence: 99%

The Use of KIT and Tryptase Expression Patterns as Prognostic Tools for Canine Cutaneous Mast Cell Tumors

et al. 2004

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“…However, Gray et al, reported that 400 mg/kg carbendazim did not cause any histopathological damage the liver of rats dosed from weaning until 105 days of age (Gray et al,1990). In contrast male mice fed 5000 mg/kg carbendazim in diet showed centrilobular hypertrophy, necrosis and swelling of liver cells (Simoes and Schoning, 1994). Igbedioh and Akinyele have observed necrotized cells and oedema in the liver of rats given benomyl (WHO/IPCS, 1993).…”

Section: Figmentioning

confidence: 99%

Toxic effects of carbendazim at low dose levels in male rats

et al. 2008

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-Carbendazim is a systemic broad-spectrum fungicide controlling a wide range of pathogens. It is also used as a preservative in paint, papermaking and leather industry, and as a preservative of fruits. In the present study, low dose intracellular effect of carbendazim was investigated employing 5, 10, 25 and 50 mM of the compound administered to male rats intradermally. Blood and liver of each animal was collected 6 hrs later to analyze serum and tissue enzyme activities, tissue lipid peroxidation and hematological and biochemical parameters. The experimental results of low dosage carbendazim use indicated augmentation of investigated parameters. However, the higher dosage of carbendazim use resulted in renormalization of investigated parameters to control levels or to values below control, providing a Ushaped hormesis type dose-response profile. Histopathological sections revealed portal vein congestion, mononuclear cell infiltration and hydropic degeneration of the liver tissue. These results indicated that carbendazim even at low dose exhibited toxicity, affected the liver and also caused specific changes in hematological and biochemical parameters in the rat.

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“…It is expressed with a more intense staining in macrophages than in monocytes but also stains granulocytes and mast cells. 22 The purpose of the current study was to characterize the histological features of the multiple atypical mucosal xanthomas observed in the oral cavity and upper gastrointestinal tract of a dog and to compare these features with those of human verruciform xanthomas.…”

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confidence: 99%

Multiple Atypical Mucosal Xanthomas in a Dog Similar to Human Verruciform Xanthoma

et al. 2009

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Abstract. A case of atypical mucosal xanthomas in a 14-year-old Shi-Tzu dog is reported. Grossly, 1-6-mm granular plaques or slightly elevated papillary nodules were found in the oral cavity (dorsal and ventral sides of the tongue, inner upper lip) and in the upper digestive tract (esophagus, stomach). Microscopically, subepithelial aggregates of large foamy cells were found in strong association with papillary epithelial hyperplasia. By immunohistochemistry, the majority of these cells tested positive for lysozyme and ACM1. The cells had cytoplasmic lipid content that stained positively with oil red O. These findings confirmed a monocyte/macrophage lineage. On the basis of macroscopic observations, microscopic changes, and the absence of a clinical metabolic defect, the condition in this dog appears similar to that of humans with oral verruciform xanthoma. The pathogenesis of these xanthomas in the dog remains obscure, although this condition appears to affect people and dogs with an Asian origin.

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Canine Mast Cell Tumors: A Comparison of Staining Techniques

Cited by 40 publications

References 26 publications

The Use of KIT and Tryptase Expression Patterns as Prognostic Tools for Canine Cutaneous Mast Cell Tumors

The Use of KIT and Tryptase Expression Patterns as Prognostic Tools for Canine Cutaneous Mast Cell Tumors

Toxic effects of carbendazim at low dose levels in male rats

Multiple Atypical Mucosal Xanthomas in a Dog Similar to Human Verruciform Xanthoma

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