Canine Lymphomas: a Morphological and Immunohistochemical Study of 55 Cases, with Observations on p53 Immunoexpression

Sueiro, Felipe Augusto Ruiz; Alessi, Antônio Carlos; Vassallo, José

doi:10.1016/j.jcpa.2004.04.002

Cited by 45 publications

(47 citation statements)

References 26 publications

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“…The majority of studies performed on canine lymphomas determined that B cell tumors are more common (51-64%) then T cell lymphomas (26-38% lymphomas in dogs) (Jagielski et al 2002, Sueiro et al 2004, Sokołowska 2005, Dzimira 2007, Guija de Arespacochaga et al 2007). The results of the present study confirmed this observation, and also indicate that B cell lymphomas are more common -more then 77% of cases.…”

Section: Discussionmentioning

confidence: 99%

Practical aspects of immunocytochemistry in canine lymphomas

Sapierzyński

2010

Polish Journal of Veterinary Sciences

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The aim of this study was to evaluate the utility of immunocytochemistry in a standard veterinary practice and to determine the immunophenotype of tumor cells in cases of multicentric lymphoma in dogs by immunocytochemical analysis of fine-needle biopsy specimens. The study was performed on cytological samples collected from 54 dogs, in which multicentric lymphoma was recognised based on clinical data, cytology or cytology and histology, and follow-up information. Diagnosis of lymphoma was established according to the updated Kiel classification. Immunocytochemical assays were conducted using commercially available antibodies to the pan T-lymphocyte marker CD3 and B cell antigen receptor complex CD79 alpha. Among all animals examined B cell lymphoma was recognized in 42/54 (77.8%) of cases, while in the remaining 12/54 (22.2%) of dogs T cell lymphoma was recognized. In 11 animals with lymphoma recognized cytologically, in which an entire lymph node was obtained for histology, the results of routine cytology and immunocytochemistry fully corresponded with findings revealed by histology and immunohistochemistry. Immunocytochemistry can be successfully conducted in smears stored at room temperature for 24 hours without changes of staining results. It can be stated that application of standard cytopathological assessment in connection with immunocytochemistry of lymph nodes samples collected from dogs with lymphoma is a method of choice for establishing final diagnosis, and avoids the need for reexamination or collection of tissue samples for histopathology and immunohistochemistry during surgical procedures in ambiguous cases.

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Section: Discussionmentioning

confidence: 99%

Practical aspects of immunocytochemistry in canine lymphomas

Sapierzyński

2010

Polish Journal of Veterinary Sciences

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“…La clasificación final del tumor se efectuó por descarte, ya que el examen IHQ de la muestra fue negativo para los anticuerpos (antihumano) contra los marcadores CD3 y CD20 (8,11).…”

Section: Hallazgos De Necropsiaunclassified

“…Los linfomas caninos presentan inmunoexpresión aberrante de antígenos CD, como CD3 y CD79a, que son los marcadores más utilizados para la determinación del linaje celular T y B, respectivamente (8). En el caso de este reporte, se realizó inmunotinción con anticuerpos para CD20, como alternativa a la utilización de anticuerpos CD79a para la identificación de linfoma de células B (9).…”

Section: Hallazgos De Necropsiaunclassified

Plasmocitoma extramedular nasal en un perro

López

Carmona

2012

Rev MVZ Córdoba

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RESUMENSe describe un caso de plasmocitoma nasal en un canino, macho entero, de raza Akita de 30 Kg de peso y veintiún meses de edad, con historia de epistaxis unilateral crónica. Se practicó examen clínico y biopsia de la neoplasia visible en la cavidad nasal izquierda. La ubicación y extensión del tumor fue determinada mediante tomografía computarizada de cabeza y cuello. Se realizaron análisis histopatológico e inmunohistoquímico (IHQ) del tejido tumoral. La tomografía computarizada evidenció una masa con densidad de tejido blando de 10 cm de longitud x 3.5 cm de diámetro y escasa captación de medio de contraste, que comprometía en su totalidad la cavidad nasal derecha y parte de la porción posterior de la coana izquierda. El análisis histopatológico reveló numerosas células redondas pleomórficas con poco citoplasma, rodeadas por trama escasa de tejido conectivo y bajo índice mitótico. En el examen IHQ la muestra fue negativa a los antígenos CD3 y CD20 para linfocitos T y B, respectivamente. Los hallazgos clínicos y de la tomografía computarizada, así como los resultados del análisis histopatológico del tejido tumoral, fueron compatibles con un plasmocitoma extramedular nasal de bajo grado de malignidad. Palabras clave: Cavidad nasal, perro, plasmocitoma, tomografía (Fuente: MeSH MEDLINE). ABSTRACTA case of nasal plasmacytoma with history of chronic unilateral epistaxis in a twenty-one month old, male, Akita dog, weighing 30 kg is described. Clinical examination and biopsy of the visible tumor in the left nasal cavity were performed. The location and extent of the tumor were determined by head and neck computer tomography. Histopathological and immune-histochemical (IHC) analyses of tumoral tissue were performed. Computer tomography showed a mass with soft tissue density of 10 cm long x 3.5 cm in diameter and low uptake of the contrast medium that entirely compromised the right nasal cavity and part of the rear portion of the left choana. The histopathological analysis revealed the presence of numerous pleomorphic round cells with little cytoplasm, surrounded by a scarce network of connective tissue, and a low mitotic index. The IHC analysis was negative for CD3 and CD20 antigens or T and B lymphocytes, respectively. Clinical and computer tomography findings, such as the results of the histopathology of the tumoral tissue were consistent with a low-grade malignancy extramedular plasmacytoma.

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“…The usefulness of these markers to differentiate histiocytic and myeloid neoplasms from lymphoma and other small cell neoplasia was demonstrated elsewhere (22) .…”

Section: Introductionmentioning

confidence: 99%

“…Veterinary medicine has progressively improved its therapeutic spectrum, especially in oncology, demanding Lymphoid markers are largely used in human pathology and have been shown to cross react in various animal species (8,9,14,22,29,30,32) . Nevertheless, the list of antibodies for use in veterinary pathology is still small.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemistry in diagnostic veterinary pathology: a critical review

Ruiz¹,

Alessi²,

Chagas³

et al. 2005

J. Bras. Patol. Med. Lab.

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key words unitermos abstractImmunohistochemistry has become a practical and widely used tool for diagnosis in human pathology since the 70's. However, its application in veterinary diagnostic pathology has not been so common, especially due to the lack of specific antibodies. To overcome this drawback, antibodies which present cross reactivity with human and animal antigens have been applied. The purpose of the present study was to test the cross reactivity of some antibodies intended for the human pathology, which may be used in animal tissues, with the help of antigen retrieval and amplification systems. In the present study it was confirmed that many of the antibodies produced for use in human histopathology might be applied in veterinary pathology. Further studies are needed to increase the list of applicability of these antibodies to different animal species. It must be stressed that in this type of study some variables, such as clone of antibody, dilution, antigen retrieval method, and detection system, have to be evaluated. Lab • v. 41 • n. 4 • p. 263-70 • agosto 2005 a more accurate diagnosis. This fact has brought to the attention of veterinary pathologists the need to improve the application of immunohistochemistry in their daily routine, following the tendency of human diagnostic pathology. Immunohistochemistry

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Canine Lymphomas: a Morphological and Immunohistochemical Study of 55 Cases, with Observations on p53 Immunoexpression

Cited by 45 publications

References 26 publications

Practical aspects of immunocytochemistry in canine lymphomas

Practical aspects of immunocytochemistry in canine lymphomas

Plasmocitoma extramedular nasal en un perro

Immunohistochemistry in diagnostic veterinary pathology: a critical review

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