Canine intestinal secretion during and after rapid distention of the small bowel

Caren, JF; Meyer, J.-U.; Grossman, M. I.

doi:10.1152/ajplegacy.1974.227.1.183

Cited by 64 publications

(31 citation statements)

References 5 publications

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“…The possibility that these fibers may have a role to play in function, particularly in the control of salt and water transport, is suggested by several pieces of evidence. Sympathetic denervation in animals induced intestinal secretion which was blocked by atropine (1,12) and the spontaneous secretion of dog Thirty-Vlella loops, and that induced by tactile stimulation, was also inhibited by atropine (2,3 changes in response to high concentrations of acetyl choline were not affected by neostigmine or atropine, confirming that they were not mediated by muscarinic cholinergic receptors.…”

Section: Methodsmentioning

confidence: 73%

See 1 more Smart Citation

In vitro behavior of human intestinal mucosa. The influence of acetyl choline on ion transport.

Isaacs¹,

Corbett²,

Riley³

et al. 1976

J. Clin. Invest.

109

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A B S T R A C T The possibility that the autonomic nervous system may influence the function of intestinal mucosa was investigated by assessing the effect of acetyl choline on ion transport in human intestine. Isolated pieces of stripped ileal mucosa were mounted in Perspex flux-chambers and bathed in isotonic glucose Ringer's solution. Acetyl choline caused a rise in mean potential difference (8.8-12.3 mV, P < 0.002) and short circuit current (287.7-417.2 A'A -cm-2, P < 0.01) (n = 12), observable at a concentration of 0.01 mM and maximal at 0.1 mM. This effect was enhanced by neostigmine and blocked by atropine. Isotopic flux determinations revealed a change from a small mean net Cl absorption (+ 0.58) to a net Cl secretion (-4.3 Aeq-cmf'-h P < 0.001) due predominantly to an increase in the serosal to mucosal unidirectional flux of Cl (10.63-14.35 ueq. cm . h-' P < 0.05) and a smaller reduction in the mucosal to serosal flux (11.22 to 10.02 /Aeq cm-' h' P < 0.05). Unidirectional and net Na transport was unaffected. A similar electrical and ion transport response was observed in a single study of two pieces of jejunal mucosa. In the absence of glucose net chloride secretion was produced and again an insignificant effect on net sodium transport was noted. Acetyl choline did not provoke a sustained effect on mucosal cyclic adenine nucleotide levels although a short-lived cyclic adenine nucleotide response was seen in some tissues 20-30 s after drug addition.These studies demonstrate that acetyl choline does influence human intestinal ion transport by stimulating chloride secretion and suggest a possible mechanism by A preliminary report of this work was presented at the British Society of Gastroenterology, Southampton, April 1975 and at the European Society for Clinical Investigation, Rotterdam, April, 1975. Received for publication 20 August 1975 and in revised formn 13 May 1976. which the parasympathetic nervous system could be concerned in the control of ion transport. INTRODUCTION It has long been thought that the autonomic nervous system may influence intestinal absorption and secretion. Sympathetic denervation in the dog and the cat caused an intestinal secretion which was blocked by atropine(1) and the spontaneous secretion of Thirty-Vella loops of dog ileum was similarly prevented by atropine (2).More recently Caren and co-workers (3) showed that the secretion provoked by tactile stimulation in such isolated loops was inhibited by atropine and hexamethonium. Pilocarpine (4) and eserine (5) caused intestinal secretion and bethanechol, a cholinergic agent, was shown to induce a net secretion of chloride in dog jejunum (6). In view of these suggestive pieces of evidence the present investigations were undertaken to examine in more detail the influence of acetyl choline on human intestinal ion transport. The results indicate that acetyl choline does influence ion transport in vitro and suggest a possible mechanism by which the parasympathetic nervous system could be involved in the control of intestina...

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Section: Methodsmentioning

confidence: 73%

“…More recently Caren and co-workers (3) showed that the secretion provoked by tactile stimulation in such isolated loops was inhibited by atropine and hexamethonium. Pilocarpine (4) and eserine (5) caused intestinal secretion and bethanechol, a cholinergic agent, was shown to induce a net secretion of chloride in dog jejunum (6).…”

mentioning

confidence: 99%

In vitro behavior of human intestinal mucosa. The influence of acetyl choline on ion transport.

Isaacs¹,

Corbett²,

Riley³

et al. 1976

J. Clin. Invest.

109

View full text Add to dashboard Cite

show abstract

“…az-2 adrenergic agonists, for example, stimulate Na and Cl absorption in several animal models (56). Reflex arcs involving cholinergic neurons, conversely, may mediate secretion induced by luminal obstruction (29). In our study, motor activity was not monitored, but significant distention of the bowel was not observed at faster flow rates because there was little change in pressure and volume.…”

Section: Discussionmentioning

confidence: 73%

“…Three mechanisms were considered whereby increased flow rate may have increased Na absorption: (a) Increased intraluminal hydrostatic pressure could act as a driving force for fluid and electrolyte absorption (27), although higher pressures could also obstruct venous outflow (21,28) or stimulate secretory processes by inducing cholinergic reflexes (29); (b) Increased intraluminal volume (surface area) could result in recruitment of epithelial transport sites (7,8); and (c) Decreased unstirred layers could facilitate access of electrolytes to epithelial transport sites (11,12). Fig.…”

Section: Methodsmentioning

confidence: 99%

Augmentation of neutral sodium chloride absorption by increased flow rate in rat ileum in vivo.

Harris¹,

Dobbins²,

Binder³

1986

J. Clin. Invest.

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Studies in intact animals have shown that intestinal solute absorption is enhanced with increasing flow rates; the mechanism of this phenomenon has not been explored in detail. We used single pass perfusions of rat ileum to study the effect of higher flow rate on electrolyte absorption. Augmenting perfusion rate from 0.5 to 5.0 ml/min resulted in increased rates of sodium (11.0±0.9 vs. 23.5±2.7 jseq/min * g) and chloride (12.1±0.8 vs. These studies demonstrate that neutral sodium chloride absorption is enhanced in rat ileum at higher flow rates, perhaps as a result of a decrease in the thickness of unstirred layers.

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“…Because net water, Na and Cl secretion occurred over a wide range of Na and Cl concentrations in luminal fluid, including the Ringer's solution, it was not possible to achieve a steady-state condition (or to observe fluid movement at a rate close to zero). Although the mechanism of secretion under this experimental condition was not elucidated, it was assumed to reflect the effect oflimited distension of the colon, which in small intestine is associated with net fluid, Na, and Cl secretion (10).…”

Section: Resultsmentioning

confidence: 99%

Demonstration of active potassium transport in the mammalian colon.

Kliger¹,

Binder²,

Bastl³

et al. 1981

J. Clin. Invest.

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Canine intestinal secretion during and after rapid distention of the small bowel

Cited by 64 publications

References 5 publications

In vitro behavior of human intestinal mucosa. The influence of acetyl choline on ion transport.

In vitro behavior of human intestinal mucosa. The influence of acetyl choline on ion transport.

Augmentation of neutral sodium chloride absorption by increased flow rate in rat ileum in vivo.

Demonstration of active potassium transport in the mammalian colon.

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