Canine Infection by Rickettsiae and Ehrlichiae in Southern Brazil

Saito, Taís B.; Cunha-Filho, Nilton A.; Pacheco, Richard C.; Ferreira, Fernando; Pappen, Felipe Geraldo; Farias, Nara Amélia da Rosa; Larsson, Carlos Eduardo; Labruna, Marcelo Bahia

doi:10.4269/ajtmh.2008.79.102

Cited by 64 publications

(60 citation statements)

References 26 publications

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“…Molecular methods demonstrated similar results for the effects of sex and age relative to infection by E. canis in Cuiaba, State of Mato Grosso (SILVA et al, 2010), and Ilheus and Itabuna, State of Bahia (CARVALHO et al, 2008). This pattern was also observed with serological assessments performed in several Brazilian cities: Cuiaba, State of Mato Grosso (SILVA et al, 2010), Monte Negro, State of Rondonia (AGUIAR et al, 2007), Patos, State of Paraiba (AZEVEDO et al, 2011), and southern cities in the State of Rio Grande do Sul (SAITO et al, 2008). Serological surveys made in the USA (RODGERS et al, 1989), Israel (HARRUS et al, 1997, and Japan (INOKUMA et al, 1999) revealed similar findings.…”

Section: Resultssupporting

confidence: 60%

Occurrence of Ehrlichia canis and Anaplasma platys in household dogs from northern Parana

Silva

Benitez

Girotto

et al. 2012

Rev. Bras. Parasitol. Vet.

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Canine monocytic ehrlichiosis caused primarily by Ehrlichia canis and canine thrombocytic anaplasmosis induced by Anaplasma platys are important emerging zoonotic tick-borne diseases of dogs. There is evidence that these pathogens can also affect humans. This study evaluated the presence of E. canis and A. platys in blood samples collected from 256 domiciled dogs in the municipality of Jataizinho, located in north region of the State of Parana, Brazil, by PCR assay. The occurrence of E. canis and A. platys was 16.4% (42/256) and 19.4% (49/256), respectively; while 5.47% (14/256) of the dogs evaluated were co-infected by these two organisms. The presence of E. canis and A. platys was not significantly associated with the variables evaluated (sex, age, outdoor access, and presence of ticks during blood collection). Infection of dogs by E. canis was associated with anemia and thrombocytopenia, while infection induced by A. platys was related only to thrombocytopenia. Canine monocytic ehrlichiosis and canine thrombocytic anaplasmosis should be included in the differential diagnoses when these hematological alterations are observed during routine laboratory evaluation of dogs.Keywords: Canine monocytic ehrlichiosis, canine thrombocytic anaplasmosis, PCR, anemia, thrombocytopenia. ResumoErliquiose monocítica canina, causada principalmente por Ehrlichia canis, e anaplasmose trombocítica canina, devida a infecção com Anaplasma platys, são importantes doenças transmitidas por carrapatos que acometem os cães, com evidências que podem também acometer o homem. O presente estudo avaliou a ocorrência desses agentes em amostras de sangue de 256 cães domiciliados na cidade de Jataizinho, na região Norte do Paraná, Brasil, utilizando a técnica da Reação em Cadeia da Polimerase (PCR). A ocorrência de E. canis e A. platys foi de 16,4% (42/256) e 19,4% (49/256), respectivamente, com 5,47% (14/256) dos animais apresentando coinfecção. Não foi observada associação significativa com as variáveis sexo, idade, acesso à rua e presença de carrapatos no momento da coleta de sangue. A infecção por E. canis teve relação com anemia e com trombocitopenia, enquanto a infecção por A. platys apresentou relação apenas com trombocitopenia. Com base nos resultados obtidos, reforçou-se a necessidade de que erliquiose e anaplasmose canina devem estar entre os diagnósticos diferenciais, quando da detecção de anemia e trombocitopenia em exames laboratoriais.

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Section: Resultssupporting

confidence: 60%

Occurrence of Ehrlichia canis and Anaplasma platys in household dogs from northern Parana

Silva

Benitez

Girotto

et al. 2012

Rev. Bras. Parasitol. Vet.

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“…The frequency of E. canis in the serum of dogs in Lavras (MG) was lower than the results observed by Silva et al (19) in Cuiaba (MT), 42.5% (108/254; IFAT ≥40), and by Souza et al (20) in Salvador (BA), 35.6% (168/472; IFAT ≥80). However, Saito et al (21) observed a prevalence of 4.8% (19/389; IFAT ≥80) in Rio Grande do Sul, which was low compared to that obtained in the present study. Importantly, high prevalence rates reported in some studies may be explained by the use of a sample population with clinical suspicion for CME, increasing the possibility of finding dogs with anti-E. canis antibodies.…”

Section: Resultscontrasting

confidence: 55%

HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

et al. 2017

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The aim of this study was to determine the prevalence of anti-Ehrlichia canis and anti-Babesia vogeli IgG antibodies in dogs and correlate this prevalence with risk factors to evaluate the relation of serological status to hematological findings. Blood samples of dogs attended from September 2011 to March 2012 at the veterinary hospital of the Federal University of Lavras, Brazil, were analyzed using an indirect fluorescence antibody test (IFAT). Of the total 160 dog serum samples, 23.7% (38 dogs; CI 95 17.7% -30.7%) were seropositive for E. canis, 40.0% (64 dogs; CI 95 40.0% -59.2%) for B. vogeli, and 5.6% (9 dogs) for both hemoparasites. None of the epidemiological variables showed a significant association (P>0.05) with seropositivity to E. canis and B. vogeli. Dogs seropositive for E. canis showed lower values for hematocrit (P<0.05). However, for the erythrogram, the platelet count, the leukogram and clinical signs, no significant difference (P>0.05) was observed between dogs that were seropositive and seronegative for E. canis or for B. vogeli. Serological results suggest that infection with E. canis and B. vogeli is endemic in the canine population in question, with a prevalence of the subclinical phase (asymptomatic) in dogs that are seropositive for ehrlichiosis or babesiosis. Keywords: babesiosis; canine; ehrlichiosis; IFAT; laboratory findings. ResumoO objetivo deste estudo foi determinar a prevalência de anticorpos IgG anti-Ehrlichia canis e antiBabesia vogeli em cães e correlacionar com a prevalência e fatores de risco para avaliar a relação do estado sorológico com os achados hematológicos. Amostras de sangue de cães coletadas de setembro de 2011 a março de 2012, no Hospital Veterinário da Universidade Federal de Lavras, Brasil, foram analisadas usando o teste de reação de imunofluorescência indireta (RIFI). Do total de amostras de soro de 160 cães, 23,7% (38 cães; IC95 17,7% -30,7%) foram soropositivos para E. canis, 40,0% (64 cães; IC95 40,0% -59,2%) para B. vogeli, e 5,6% (9 cães) para os dois hemoparasitas. Nenhuma das variáveis epidemiológicas mostrou associação significativa (P> 0,05) com a soropositividade para E. canis e B. vogeli. Cães soropositivos para E. canis mostraram valores médios mais baixos para hematócrito (P<0,05). No entanto, para o eritrograma, a contagem de plaquetas, o leucograma e os sinais clínicos, nenhuma diferença significativa (P> 0,05) foi observada entre cães soropositivos e soronegativos para E. canis ou para B. vogeli. Os resultados sorológicos deste estudo sugerem que a Cienc. anim. bras., Goiânia, v.18, 1-9, e-36095, 2017 2 FONSECA, J.P., et al.infecção por E. canis e B. vogeli é endêmica na população canina em questão, com uma prevalência da fase subclínica (assintomática) em cães soropositivos para erliquiose ou babesiose.

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“…by IFA, and R. parkeri was the causative agent in 100/389 (25.7%) dogs (SAITO et al, 2008). We hypothesize that low titers may be a result of a less recent or severe infection, probably due to low or non-pathogenic Rickettsia species.…”

Section: Discussionmentioning

confidence: 99%

“…in rural and urban areas in Northern Brazil. Although forest areas are common in the region studied (dog origin) (Figure 2) and may favor tick proliferation (SAITO et al, 2008), no positive animal was found in the rural area. The fact that seropositive dogs were found only in urban areas may be explained by tick distribution associated to higher concentration of host species in these areas.…”

Section: Discussionmentioning

confidence: 99%

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Seroprevalence of Rickettsia bellii and Rickettsia felis in dogs, São José dos Pinhais, State of Paraná, Brazil

Fortes

Silveira

Moraes‐Filho

et al. 2010

Rev. Bras. Parasitol. Vet.

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Seroprevalence of Rickettsia bellii and AbstractBrazilian spotted fever (BSF) is a vector-borne zoonosis caused by Rickettsia rickettsii bacteria. Dogs can be host sentinels for this bacterium. The aim of the study was to determine the presence of antibodies against Rickettsia spp. in dogs from the city of São José dos Pinhais, State of Paraná, Southern Brazil, where a human case of BSF was first reported in the state. Between February 2006 and July 2007, serum samples from 364 dogs were collected and tested at 1:64 dilutions by indirect immunofluorescence assay (IFA) against R. rickettsii and R. parkeri. All sera that reacted at least to one of Rickettsia species were tested against the six main Rickettsia species identified in Brazil: R. rickettsii, R. parkeri, R. bellii, R. rhipicephali, R. amblyommii and R. felis. Sixteen samples (4.4%) reacted to at least one Rickettsia species. Among positive animals, two dogs (15.5%) showed suggestive titers for R. bellii exposure. One sample had a homologous reaction to R. felis, a confirmed human pathogen. Although Rickettsia spp. circulation in dogs in the area studied may be considered at low prevalence, suggesting low risk of human infection, the present data demonstrate for the first time the exposure of dogs to R. bellii and R. felis in Southern Brazil.Keywords: Brazilian spotted fever, Rickettsia rickettsii, sentinels, indirect immunofluorescence assay. ResumoA febre maculosa brasileira (FMB) é uma zoonose veiculada por carrapatos e causada pela bactéria Rickettsia rickettsii, podendo os cães ser hospedeiros sentinelas para essa bactéria. O objetivo do estudo foi determinar a presença de anticorpos contra Rickettsia spp. em cães de São José dos Pinhais, estado do Paraná, Sul do Brasil. Entre fevereiro de 2006 e julho de 2007, amostras séricas de 364 cães foram coletadas e testadas na diluição de 1:64 por Reação de Imunofluorescência Indireta (RIFI) contra R. rickettsii e R. parkeri. Todos os soros reagentes para pelo menos uma espécie de Rickettsia foram testados contra as seis principais espécies de Rickettsia identificadas no Brasil: R. rickettsii, R. parkeri, R. bellii, R. rhipicephali, R. amblyommii e R. felis. Dezesseis amostras (4,4%) reagiram para pelo menos uma espécie de Rickettsia. Dos animais positivos, dois cães (15,5%) apresentaram títulos sugestivos de exposição a R. bellii. Uma amostra apresentou reação homóloga frente à R. felis, um agente patogênico confirmado para seres humanos. Muito embora os resultados demonstrem uma baixa prevalência de Rickettsia spp. em cães, sugerindo um baixo risco de infecção humana, este estudo relatou pela primeira vez a evidência de exposição a R. bellii e R. felis em cães no Sul do Brasil.Palavras-chave: Febre maculosa brasileira, Rickettsia rickettsii, sentinelas, reação de imunofluorescência indireta.

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Canine Infection by Rickettsiae and Ehrlichiae in Southern Brazil

Cited by 64 publications

References 26 publications

Occurrence of Ehrlichia canis and Anaplasma platys in household dogs from northern Parana

Occurrence of Ehrlichia canis and Anaplasma platys in household dogs from northern Parana

HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

Seroprevalence of Rickettsia bellii and Rickettsia felis in dogs, São José dos Pinhais, State of Paraná, Brazil

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