Canine herpesvirus 47kD and mutated 41kD glycoproteins are responsible for hemagglutination.

Xuan, Xuenan; Horimoto, Taisuke; Limcumpao, Joselito A.; Nemoto, Koji; Tohya, Yukinobu; Azetaka, Masayuki; Takahashi, Eiji; Mikami, Takeshi

doi:10.1292/jvms1939.52.899

Cited by 12 publications

(16 citation statements)

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“…Similarly, the host range of CHV is also very narrow [4]. A 47 kDa glycoprotein of CHV being responsible for HA was encoded by CHV gD gene [15,24], and cross-reacted with FHV-1 gD by an MAb 25C9 against FHV-1 gD [12]. This MAb possessed inhibition activities against both HAs by FHV-1 gD and CHV gp47 [12].…”

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confidence: 99%

Adhesion of Insect Cells Expressing the Feline Herpesvirus Type 1 Hemagglutinin(gD) to Feline Cell Lines.

Maeda

Ono

Kawaguchi

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. Feline herpesvirus type 1 (FHV-1) gD-expressing Sf9 cells adhered to two feline cell lines, but not to the porcine, bovine, or canine cell lines tested. In addition, this adhesion activity was inhibited by a monoclonal antibody against FHV-1 gD. These results showed that the FHV-1 gD might bind to a specific-molecule(s) on the surface of feline cell lines. We discussed a possible importance of the FHV-1 gD in host cell restriction to FHV-1 infection. -KEY WORDS: adhesion, FHV-1 gD, hemagglutinin.

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confidence: 99%

Adhesion of Insect Cells Expressing the Feline Herpesvirus Type 1 Hemagglutinin(gD) to Feline Cell Lines.

Maeda

Ono

Kawaguchi

et al. 1997

The Journal of Veterinary Medical Science

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“…The CHV gD expressed by recombinant baculovirus in insect cells appeared to correspond in size to the partially glycosylated form of authentic gD, which has an apparent molecular mass of 47 kDa [34]. This suggests that recombinant gD undergoes glycosylation but no further processing to complex forms.…”

Section: Discussionmentioning

confidence: 91%

“…Viruses and cells: CHV YP2 strain [34] was propagated in Madin-Darby canine kidney (MDCK) cells which were grown in Dulbecco's modified Eagle's medium (DMEM) (SIGMA, MO, U.S.A.) supplemented with 5% heatinactivated fetal calf serum (FCS) and 100 units of penicillin Canine herpesvirus (CHV) belongs to family Herpesviridae, subfamily Alphaherpesvirinae [26]. CHV causes a fatal hemorrhagic disease in neonatal puppies and an upper respiratory tract infection in adult dogs [3].…”

Section: Methodsmentioning

confidence: 99%

“…All of the MAbs against gD possessed complement-independent VN activity [35]. In addition, four of the five MAbs against CHV gD inhibited hemagglutination (HA) activity of CHV against canine red blood cells (RBC) [34].…”

Section: Methodsmentioning

confidence: 99%

“…As CHV gD consists of 345 amino acid residues with a predicted MW of approximately 38 kDa [14], the estimated MW of the TM-treated gD seems to be reasonable. HAD and HA tests: We reported that CHV has HA activity, which is inhibited by four MAbs against CHV gD, and that CHVgD might be a hemagglutinin [23,34]. Recently, Xuan et al [33] reported that CHVgD expressed by recombinant vaccinia virus agglutinated canine RBC.…”

Section: Expression Of Chv Gd In Cos-7 Cellsmentioning

confidence: 99%

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Characterization of Canine Herpesvirus Glycoprotein D (Hemagglutinin).

Maeda

Xuan

Kawaguchi

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. Glycoprotein D (gD) of canine herpesvirus (CHV) YP2 strain was expressed in COS-7 and insect (Spodoptera frugiperda; Sf9) cells. The gDs expressed in COS-7 and Sf9 cells reacted with a panel of monoclonal antibodies (MAbs) against CHV gD (hemagglutinin) and an MAb 25C9 against feline herpesvirus type 1 (FHV-1) gD by indirect immunofluorescence assay, and possessed a molecular weight (MW) of approximately 51-55 and 41-46 kilodalton (kDa), respectively, when examined by immunoblot analysis. After treatment with tunicamycin, the MW of the gD expressed in Sf9 cells became approximately 37 kDa. By hemadsorption (HAD) tests using canine or feline red blood cells (RBC), COS-7 cells expressing CHV gD adsorbed only canine RBC, but not feline RBC, whereas control COS-7 cells expressing FHV-1 gD adsorbed feline RBC, but not canine RBC. By hemagglutination (HA) tests, lysates of Sf9 cells expressing CHV gD agglutinated canine RBC, but not feline RBC. These HA and HAD activities were inhibited by HA-inhibition MAbs against CHV gD. Control lysates of Sf9 cells expressing FHV-1 gD agglutinated only feline RBC. Serum from mice inoculated with lysates of Sf9 cells expressing CHV gD possessed a high titer of virus-neutralizing activities against CHV infection. These results indicated that CHV gD is structurally similar to FHV-1 gD, but is functionally different from FHV-1 gD. -KEY WORDS : canine herpesvirus, feline herpesvirus type 1, glycoprotein D, hemagglutinin.

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Glycoprotein-specific immune response in canine herpesvirus infection

Xuan

Horimoto

Limcumpao

et al. 1992

Archives of Virology

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Sera from dogs which were infected with canine herpesvirus (CHV) were analyzed for their serological reactivities against virus-specific glycoproteins (gps). By sequential immunoblot analysis using sera from experimentally infected dogs, it was found that the antibody response to gp 145/112 appeared first followed by responses to gp47 and gp80. In addition, all sera from naturally infected dogs which showed neutralizing activity to CHV reacted with gp 145/112, whereas 77% and 70% reacted with gp47 and gp80, respectively. Furthermore, some of the sera also cross-neutralized feline herpesvirus type 1 (FHV-1) and reacted with gp 143/108 of FHV-1, indicating that gp 145/112 of CHV induced cross-neutralizing antibody response to FHV-1.

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Canine herpesvirus 47kD and mutated 41kD glycoproteins are responsible for hemagglutination.

Cited by 12 publications

References 0 publications

Adhesion of Insect Cells Expressing the Feline Herpesvirus Type 1 Hemagglutinin(gD) to Feline Cell Lines.

Adhesion of Insect Cells Expressing the Feline Herpesvirus Type 1 Hemagglutinin(gD) to Feline Cell Lines.

Characterization of Canine Herpesvirus Glycoprotein D (Hemagglutinin).

Glycoprotein-specific immune response in canine herpesvirus infection

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