Canine Distemper Virus Associated with a Lethal Outbreak in Monkeys Can Readily Adapt To Use Human Receptors

Sakai, Kouji; Yoshikawa, Tomoki; Seki, Fumio; Fukushi, Shuetsu; Tahara, Maino; Nagata, Noriyo; Ami, Yasushi; Mizutani, Tetsuya; Kurane, Ichiro; Yamaguchi, Ryoji; Hasegawa, Hideki; Saijo, Masayuki; Komase, Katsuhiro; Morikawa, Shigeru; Takeda, Makoto

doi:10.1128/jvi.03479-12

Cited by 70 publications

(57 citation statements)

References 27 publications

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“…However, distemper has been known to occur in vaccinated dogs and outbreaks in various other hosts naturally infected, such as non-human primates have been investigated (BEINEkE et al, 2009;SAkAI et al, 2013). The definitive diagnosis of distemper often requires the laboratory tests to confirm disease without the typical symptoms and the similarity of clinical signs with other ailments.…”

Section: Resultsmentioning

confidence: 99%

Canine distemper virus detection by different methods of One-Step RT-qPCR

et al. 2016

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Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Canine Distemper Virus. Using the kit that showed better performance, two systems of Realtime RT-PCR (RT-qPCR) assays were tested and compared for analytical sensitivity to Canine Distemper RESUMO Três kits comerciais de One-Step RT-qPCR foram avaliados para o diagnóstico molecular do Vírus da Cinomose Canina.Utilizando o kit que apresentou melhor desempenho, dois sistemas de RT-PCR em tempo real (RT-qPCR) foram comparados quanto à sensibilidade analítica na detecção do RNA do Vírus da Cinomose Canina:One-Step RT-qPCR (Sistema A) e One-Step RT-qPCR seguido da NESTED-qPCR (Sistema B).Os limites de detecção dos dois sistemas foram determinados utilizando diluição

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Section: Resultsmentioning

confidence: 99%

Canine distemper virus detection by different methods of One-Step RT-qPCR

et al. 2016

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“…Besides the possible antigenic relevance of these changes, we speculate that outcomes from these changes might also be at the base of the molecular plasticity that has allowed CDV to become such a successful viral agent with a continuously growing host range (19,(34)(35)(36).…”

Section: Discussionmentioning

confidence: 99%

Identification of Amino Acid Substitutions with Compensational Effects in the Attachment Protein of Canine Distemper Virus

Sattler

Khosravi

Avila

et al. 2014

J Virol

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The hemagglutinin (H) gene of canine distemper virus (CDV) encodes the receptor-binding protein. This protein, together with the fusion (F) protein, is pivotal for infectivity since it contributes to the fusion of the viral envelope with the host cell membrane. Of the two receptors currently known for CDV (nectin-4 and the signaling lymphocyte activation molecule [SLAM]), SLAM is considered the most relevant for host susceptibility. To investigate how evolution might have impacted the host-CDV interaction, we examined the functional properties of a series of missense single nucleotide polymorphisms (SNPs) naturally accumulating within the H-gene sequences during the transition between two distinct but related strains. The two strains, a wild-type strain and a consensus strain, were part of a single continental outbreak in European wildlife and occurred in distinct geographical areas 2 years apart. The deduced amino acid sequence of the two H genes differed at 5 residues. A panel of mutants carrying all the combinations of the SNPs was obtained by site-directed mutagenesis. The selected mutant, wild type, and consensus H proteins were functionally evaluated according to their surface expression, SLAM binding, fusion protein interaction, and cell fusion efficiencies. The results highlight that the most detrimental functional effects are associated with specific sets of SNPs. Strikingly, an efficient compensational system driven by additional SNPs appears to come into play, virtually neutralizing the negative functional effects. This system seems to contribute to the maintenance of the tightly regulated function of the Hgene-encoded attachment protein. IMPORTANCETo investigate how evolution might have impacted the host-canine distemper virus (CDV) interaction, we examined the functional properties of naturally occurring single nucleotide polymorphisms (SNPs) in the hemagglutinin gene of two related but distinct strains of CDV. The hemagglutinin gene encodes the attachment protein, which is pivotal for infection. Our results show that few SNPs have a relevant detrimental impact and they generally appear in specific combinations (molecular signatures). These drastic negative changes are neutralized by compensatory mutations, which contribute to maintenance of an overall constant bioactivity of the attachment protein. This compensational mechanism might reflect the reaction of the CDV machinery to the changes occurring in the virus following antigenic variations critical for virulence.

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“…Nectin-4 is highly-conserved and does not appear to enforce species-specific limitations in morbilliviruses, since canine distemper virus (CDV) can utilize human Nectin-4 with no adaptation (Bieringer et al 2013; Sakai et al 2013b). However, nectin-4 is only required for morbillivirus exit from the host; indeed, recombinant MeV that is selectively blind for nectin-4 can infect and cause primary disease in non-human primates, but is unable to transmit or be shed (Frenzke et al 2013).…”

Section: Virus-host Molecular Interactions Affecting Paramyxovirusmentioning

confidence: 99%

Zoonotic Potential of Emerging Paramyxoviruses

Thibault

Watkinson

Moreira-Soto

et al. 2017

Advances in Virus Research

107

102

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The risk of spillover of enzootic paramyxoviruses, and the susceptibility of recipient human and domestic animal populations, are defined by a broad collection of ecological and molecular factors that interact in ways that are not yet fully understood. Nipah and Hendra viruses were the first highly-lethal zoonotic paramyxoviruses discovered in modern times, but other paramyxoviruses from multiple genera are present in bats and other reservoirs that have unknown potential to spill over into humans. We outline our current understanding of paramyxovirus reservoir hosts and the ecological factors that may drive spillover, and we explore the molecular barriers to spillover that emergent paramyxoviruses may encounter. By outlining what is known about enzootic paramyxovirus receptor usage, mechanisms of innate immune evasion, and other host-specific interactions, we highlight the breadth of unexplored avenues that may be important in understanding paramyxovirus emergence.

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Canine Distemper Virus Associated with a Lethal Outbreak in Monkeys Can Readily Adapt To Use Human Receptors

Cited by 70 publications

References 27 publications

Canine distemper virus detection by different methods of One-Step RT-qPCR

Canine distemper virus detection by different methods of One-Step RT-qPCR

Identification of Amino Acid Substitutions with Compensational Effects in the Attachment Protein of Canine Distemper Virus

Zoonotic Potential of Emerging Paramyxoviruses

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