Canine discrimination of ovarian cancer through volatile organic compounds

Kane, Sarah A.; Lee, Y E; Essler, L J; Mallikarjun, Amritha; Preti, George; Plymouth, Victoria L.; Verta, Alec; DeAngelo, Annemarie; Otto, Cynthia M.

doi:10.1016/j.talanta.2022.123729

Cited by 9 publications

(4 citation statements)

References 26 publications

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“…In future studies, researchers can also utilize dogs’ CWD detection data to help narrow down characteristic VOC biomarkers of CWD; these biomarkers could then be confirmed with an analytical instrument method such as GC-MS (gas chromatography/mass spectrometry) [ 53 ]. This information could aid in the development of a mechanical/electronic nose for CWD detection.…”

Section: Discussionmentioning

confidence: 99%

Canine detection of chronic wasting disease (CWD) in laboratory and field settings

Mallikarjun

Swartz

Kane

et al. 2023

Prion

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Chronic wasting disease (CWD) is a fatal transmissible spongiform encephalopathy that affects both free-ranging and farmed cervid species, including mule deer, white-tailed deer, and elk ( Odocoileus hemionus, Odocoileus virginianus , and Cervus canadensis ). Due to the long incubation period and variability of clinical signs, CWD can expand and spread to new areas before they reach diagnostically detectable levels. Antemortem testing methods currently available can be difficult to obtain and to be applied to the large numbers required for adequate surveillance. However, key volatile biomarkers could be harnessed for non-invasive antemortem surveillance. Detection dogs are the most effective tool currently available for volatile detection; dogs can effectively complete wildlife surveys at rates surpassing that of humans. This study is the first to demonstrate that trained detection dogs can be used as an antemortem test for CWD. First, we trained three dogs to differentiate between CWD-positive and CWD-negative white-tailed deer faeces in a laboratory setting. Dogs spent significantly more time at the positive sample than the negative samples, suggesting that they differentiated between the positive and negative volatile signatures. We then trained the same dogs to search for CWD-positive faecal samples in a more naturalistic field setting. In the field, dogs found 8/11 CWD-positive samples and had an average false detection rate of 13%. These results suggest that dogs can be trained to differentiate CWD-positive faeces from CWD-negative faeces in both laboratory and field settings. Future studies will compare canine accuracy to other antemortem methods, as well as improved canine training methods.

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Section: Discussionmentioning

confidence: 99%

Canine detection of chronic wasting disease (CWD) in laboratory and field settings

Mallikarjun

Swartz

Kane

et al. 2023

Prion

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“…This is important as it has been demonstrated in certain disease conditions, that several metabolic changes may occur, impacting an individual's VOC profile, when they become unwell ( 42 ). When considering other diseases and infections, previous studies have demonstrated dogs' abilities to specifically detect target samples of interest while ignoring similar conditions or samples indicative of “ill-health” unrelated to the target condition ( 36 , 47 , 48 ). These findings are well aligned with our observations.…”

Section: Discussionmentioning

confidence: 99%

Dogs can detect an odor profile associated with Staphylococcus aureus biofilms in cultures and biological samples

Ramos,

Chang,

Wilson

et al. 2024

Front. Allergy

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IntroductionThe study investigated the utilization of odor detection dogs to identify the odor profile of Staphylococcus aureus (S. aureus) biofilms in pure in vitro samples and in in vivo biosamples from animals and humans with S. aureus periprosthetic joint infection (PJI). Biofilms form when bacterial communities aggregate on orthopedic implants leading to recalcitrant infections that are difficult to treat. Identifying PJI biofilm infections is challenging, and traditional microbiological cultures may yield negative results even in the presence of clinical signs.MethodsDogs were trained on pure in vitro S. aureus biofilms and tested on lacrimal fluid samples from an in vivo animal model (rabbits) and human patients with confirmed S. aureus PJI.ResultsThe results demonstrated that dogs achieved a high degree of sensitivity and specificity in detecting the odor profile associated with S. aureus biofilms in rabbit samples. Preliminary results suggest that dogs can recognize S. aureus volatile organic compounds (VOCs) in human lacrimal fluid samples.DiscussionTraining odor detection dogs on in vitro S. aureus, may provide an alternative to obtaining clinical samples for training and mitigates biosecurity hazards. The findings hold promise for culture-independent diagnostics, enabling early disease detection, and improved antimicrobial stewardship. In conclusion, this research demonstrates that dogs trained on in vitro S. aureus samples can identify the consistent VOC profile of PJI S. aureus biofilm infections. The study opens avenues for further investigations into a retained VOC profile of S. aureus biofilm infection. These advancements could revolutionize infectious disease diagnosis and treatment, leading to better patient outcomes and addressing the global challenge of antimicrobial resistance.

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“…Current research demonstrates that dogs can be trained to detect hypoglycemia [ 13 ], lung cancer [ 14 ], ovarian cancer [ 15 , 16 ], prostate cancer [ 17 ], and urinary tract infections [ 18 ]. More recently, several research groups have trained dogs to identify signature volatile organic compounds (VOCs) of SARS-CoV-2 in a variety of mediums; including pharyngeal secretions [ 19 ], urine [ 20 ], saliva [ 20 ], sweat [ 21 , 22 ], and breath [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…Various works have been published on the use of HS-SPME-GC-MS for the detection and characterization of VOCs from human specimens, including sweat, blood, saliva, urine, breath, hair, and fingernails (see [ 34 ] for a review). Complementary detection of VOCs by biosensors (dogs) and analytical instrumentation (HS-SPME-GC-MS) can narrow down areas of volatile biomarkers [ 16 ] and allow for further characterization of these unique VOC profiles, providing a critical step forward for the detection of COVID-19.…”

Section: Introductionmentioning

confidence: 99%

The Use of Biological Sensors and Instrumental Analysis to Discriminate COVID-19 Odor Signatures

et al. 2022

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The spread of SARS-CoV-2, which causes the disease COVID-19, is difficult to control as some positive individuals, capable of transmitting the disease, can be asymptomatic. Thus, it remains critical to generate noninvasive, inexpensive COVID-19 screening systems. Two such methods include detection canines and analytical instrumentation, both of which detect volatile organic compounds associated with SARS-CoV-2. In this study, the performance of trained detection dogs is compared to a noninvasive headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) approach to identifying COVID-19 positive individuals. Five dogs were trained to detect the odor signature associated with COVID-19. They varied in performance, with the two highest-performing dogs averaging 88% sensitivity and 95% specificity over five double-blind tests. The three lowest-performing dogs averaged 46% sensitivity and 87% specificity. The optimized linear discriminant analysis (LDA) model, developed using HS-SPME-GC-MS, displayed a 100% true positive rate and a 100% true negative rate using leave-one-out cross-validation. However, the non-optimized LDA model displayed difficulty in categorizing animal hair-contaminated samples, while animal hair did not impact the dogs’ performance. In conclusion, the HS-SPME-GC-MS approach for noninvasive COVID-19 detection more accurately discriminated between COVID-19 positive and COVID-19 negative samples; however, dogs performed better than the computational model when non-ideal samples were presented.

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Canine discrimination of ovarian cancer through volatile organic compounds

Cited by 9 publications

References 26 publications

Canine detection of chronic wasting disease (CWD) in laboratory and field settings

Canine detection of chronic wasting disease (CWD) in laboratory and field settings

Dogs can detect an odor profile associated with Staphylococcus aureus biofilms in cultures and biological samples

The Use of Biological Sensors and Instrumental Analysis to Discriminate COVID-19 Odor Signatures

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