Canine dendritic cells from peripheral blood and lymph nodes

Generation of Canine Dendritic Cells from Peripheral Blood Mononuclear Cells

“…This procedure is easy to perform and can be applied to samples from clinical cases. Canine DCs in lymph nodes or epidermis Langerhans cells are morphologically similar to human DCs and positive for esterase staining [15,32]. They display surface antigen CD1, CD8, CD11a, CD11c and MHC class II [1, 12, 29, 30].…”

“…Further maturation can be induced by stimulation with TNF-α, lipopolysaccharides (LPS) or CD40L [2,40]. Several studies have demonstrated that canine DCs could be directly purified from blood or lymph nodes by densitygradient centrifugation procedures [3,15,22]. These canine DCs are nonadherent cells characterized by very prominent, long dendritic processes morphologically identical to those of humans.…”

“…These canine DCs are nonadherent cells characterized by very prominent, long dendritic processes morphologically identical to those of humans. They also expressed surface molecules such as CD1, CD11 and MHC class II similar to those of human DCs [1,15,30], but the number of DC isolated was not enough for DC-based immunotherapy. Recently, Hagglund et al demonstrated that cells with a DC-like phenotype and functions could be cultured from canine CD34 + bone marrow cells in the presence of human GM-CSF and TNF-α, but they failed to induce DCs from peripheral blood [16].…”

Langerhans Cells in Feline Foetal Epidermis - Immunohistochemical Study of Spatial Distribution

“…LC stimulate proliferation of important T-helper lymphocytes by antigen presentation on their surface, thus inducing also cytotoxic T-lymphocytes to moderate the allogeneic reaction. LC produce interleukin 1 and other cytokines, moreover, they are capable of phagocytosis and contain many different enzymes (Goodell et al 1985).…”

“…LC stimulate proliferation of important T-helper lymphocytes by antigen presentation on their surface, thus inducing also cytotoxic T-lymphocytes to moderate the allogeneic reaction. LC produce interleukin 1 and other cytokines, moreover, they are capable of phagocytosis and contain many different enzymes (Goodell et al 1985).LC stem cells originate from pluripotent hematopoietic progenitor cells CD34+ in the bone marrow. Recent results identified monocytes with a high expression of Gr-1(Ly-6c/G) marker as the direct precursors for LC in vivo and showed the importance of the colony-…”