Canine amniotic membrane mesenchymal stromal/stem cells: Isolation, characterization and differentiation

Borghesi, Jéssica; Lima, Mariana Ferreira; Mario, Lara Carolina; Anunciação, Adriana Raquel de Almeida da; Rabêlo, Ana Carolina Silveira; Silva, Marcella Giancoli Kato Cano da; Fernandes, Fernanda Dreux Miranda; Miglino, María Angélica; Carreira, Ana Cláudia Oliveira; Favaron, Phelipe Oliveira

doi:10.1016/j.tice.2019.04.007

Cited by 15 publications

(15 citation statements)

References 40 publications

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“…They are non-teratogenic [ 55 ], pluripotent, and also have regenerative properties and low immunogenicity [ 31 , 34 , 55 ]. Canine MSCs derived from the amniotic membrane have recently been isolated and proved to have the same characteristics, biosafety, and properties as other MSCs, which make them a good candidate for clinical trials [ 32 , 56 ]. The composition and potential use of cAMSC-CM have not been studied yet, but since CM contains cell paracrine factors [ 37 ], the application of cAMSC-CM in clinical studies seems to be promising.…”

Section: Discussionmentioning

confidence: 99%

“…They also secrete anti-inflammatory molecules and growth factors that protect cells from apoptosis when exposed to injuries [ 27 , 28 ]. Amniotic membrane-derived MSCs (AMSCs) have been isolated in dogs and humans [ 29 , 30 ]; although human AMSCs already proved to be useful in regenerative medicine [ 31 ], the use of canine AMSCs in this field have only been suggested but never applied [ 32 , 33 ]. In comparison with other stem cells, AMSCs are easier to obtain and isolate, which make them an ideal candidate for clinical trials [ 31 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

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Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters

Mahiddine

Kim

Qamar

et al. 2020

Animals

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This study investigated the effects of conditioned medium (CM) from canine amniotic membrane-derived MSCs (cAMSCs) on dog sperm cryopreservation. For this purpose, flow cytometry analysis was performed to characterize cAMSCs. The CM prepared from cAMSCs was subjected to proteomic analysis for the identification of proteins present in the medium. Sperm samples were treated with freezing medium supplemented with 0%, 5%, 10%, and 15% of the CM, and kinetic parameters were evaluated after 4–6 h of chilling at 4 °C to select the best concentration before proceeding to cryopreservation. Quality-related parameters of frozen–thawed sperm were investigated, including motility; kinetic parameters; viability; integrity of the plasma membrane, chromatin, and acrosome; and mitochondrial activity. The results showed that 10% of the CM significantly enhanced motility, viability, mitochondrial activity, and membrane integrity (p < 0.05); however, the analysis of chromatin and acrosome integrity showed no significant differences between the treatment and control groups. Therefore, we concluded that the addition of 10% CM derived from cAMSC in the freezing medium protected dog sperm during the cryopreservation process.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters

Mahiddine

Kim

Qamar

et al. 2020

Animals

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“…AF-MSCs are derived from the amniotic membrane and amniotic fluid of the embryos, which protect embryos and help maintaining proper development of fetal organs, and are usually discarded after childbirth. Except for the characteristics of MSCs, AF-MSCs have unique traits such as non-tumorigenicity and low immunogenicity, for which AF-MSCs are widely used in regenerative medicine [24].…”

Section: Amniotic Fluid-derived Mesenchymal Stem Cells (Af-mscs)mentioning

confidence: 99%

The cell repair research of spinal cord injury: a review of cell transplantation to treat spinal cord injury

Zhang

Wang

Song

2019

Journal of Neurorestoratology

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Through retrospective analysis of the literature on the cell repair of spinal cord injury worldwide, it is found that the mechanism of cell transplantation repairing spinal cord injury is mainly to replace damaged neurons, protect host neurons, prevent apoptosis, promote axonal regeneration and synapse formation, promote myelination, and secrete trophic factors or growth factors to improve microenvironment. A variety of cells are used to repair spinal cord injury. Stem cells include multipotent stem cells, embryonic stem cells, and induced pluripotent stem cells. The multipotent stem cells are mainly various types of mesenchymal stem cells and neural stem cells. Non-stem cells include olfactory ensheathing cells and Schwann cells. Transplantation of inhibitory interneurons to alleviate neuropathic pain in patients is receiving widespread attention. Different types of cell transplantation have their own advantages and disadvantages, and multiple cell transplantation may be more helpful to the patient’s functional recovery. These cells have certain effects on the recovery of neurological function and the improvement of complications, but further exploration is needed in clinical application. The application of a variety of cell transplantation, gene technology, bioengineering and other technologies has made the prospect of cell transplantation more extensive. There is a need to find a safe and effective comprehensive treatment to maximize and restore the patient’s performance.

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“…At present, in human clinical medicine, a variety of clinical trials with human MSCs are ongoing for the treatment of immunological diseases and degenerative diseases [6][7][8][9]. Currently, it has been reported that MSCs could be isolated from various tissues other than bone marrow [10], such as adipose [11], umbilical cord [12], dental pulp [13], placenta [14] and amniotic membrane [15]. Adipose-derived Mesenchymal stem cells (AD-MSCs) have gained in popularity due to the ease and abundance of harvesting adipose tissue, greater capacity of self-renewal multipotency and paracrine immunomodulatory [11,[16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Manufacturing and Banking Canine Adipose-Derived Mesenchymal Stem Cells for Veterinary Clinical Application

Luo

Li²,

Chen

et al. 2020

Preprint

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BACKGROUND: Mesenchymal stem cells (MSCs) have generated a great amount of interest in recent years as a novel therapeutic application for improving the quality of pet life and helping them free from painful conditions and diseases. It has now become critical to address the challenges related to the safety and efficacy of MSCs expanded in vitro. In this study, we establish a standardized process for manufacture of canine adipose-derived MSCs (AD-MSCs), including tissue sourcing, cell isolation and culture, cryopreservation, thawing and expansion, quality control and testing, and evaluate the safety and efficacy of those cells for clinical applications. RESULTS: After expansion, the viability of AD-MSCs manufactured under our standardized process was above 90 %. Expression of surface markers and differentiation potential was consistent with ISCT standards. Sterility, mycoplasma, and endotoxin tests were consistently negative. No adverse events were noted in two cases treated with intravenously AD-MSCs. CONCLUSION: Herein we demonstrated the establishment of a feasible bioprocess for manufacturing and banking canine AD-MSCs for veterinary clinical use.

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Canine amniotic membrane mesenchymal stromal/stem cells: Isolation, characterization and differentiation

Cited by 15 publications

References 40 publications

Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters

Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters

The cell repair research of spinal cord injury: a review of cell transplantation to treat spinal cord injury

Manufacturing and Banking Canine Adipose-Derived Mesenchymal Stem Cells for Veterinary Clinical Application

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