‘Candidatus Viridilinea mediisalina’, a novel phototrophic Chloroflexi bacterium from a Siberian soda lake

Gaisin, Vasil A.; Burganskaya, Ekaterina I; Grouzdev, Denis S.; Ашихмин, А. А.; Kostrikina, N. A.; Bryantseva, I. A.; Koziaeva, Veronika; Горленко, В. М.

doi:10.1093/femsle/fnz043

Cited by 18 publications

(14 citation statements)

References 30 publications

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“…Their presence in C. aggregans and "Ca. Viridilinea mediisalina" has been detected previously by negative stain TEM (Hanada et al, 1995;Gaisin et al, 2019a). Biogenesis of the chlorosomes is predicted in many Chloroflexales species owing to the presence of a bchK gene ( Supplementary Table S1), which encodes a bacteriochlorophyll c synthase (Frigaard et al, 2005).…”

Section: Chlorosomesmentioning

confidence: 89%

“…When imaged by negative stain TEM, gas vesicles have only been detected in mesophilic Chloroflexales represented by "Ca. Viridilinea mediisalina" (Gorlenko and Pivovarova, 1977;Keppen et al, 1994;Gorlenko et al, 2014;Gaisin et al, 2019a). Indeed, genes that are crucial for normal gas vesicle biogenesis, gvpA and gvpN (Tashiro et al, 2016), are only present in genomes of mesophilic species ( Supplementary Table S1).…”

Section: Gas Vesiclesmentioning

confidence: 99%

“…As of today, ultrastructural data on Chloroflexales bacteria are relatively scarce. Indeed, the discovery and description of ultrastructural traits in Chloroflexales bacteria have been confined to those readily detected by “conventional” electron microscopy, such as negative stain transmission electron microscopy (TEM) ( Pierson and Castenholz, 1974 ; Gorlenko and Pivovarova, 1977 ; Keppen et al, 1994 ; Hanada et al, 1995 , 2002 ; Gaisin et al, 2019a , b ). Although very insightful, this method usually does not expose the finer ultrastructural complexity of biological specimens.…”

Section: Introductionmentioning

confidence: 99%

“…Here, we used cryo-ET and cryo-FIB milling followed by cryo-ET to study the ultrastructural organization of the thermophilic Roseiflexus castenholzii , thermophilic Chloroflexus aggregans , and the recently described mesophilic bacterium “ Ca . Viridilinea mediisalina” ( Hanada et al, 1995 , 2002 ; Gaisin et al, 2019a ). We chose these species because each represents one of the three main lineages within the Chloroflexales order: bacteriochlorophyll a -containing Roseiflexus -related members, bacteriochlorophyll a and c -containing Chloroflexus -related members, and a group of mesophilic bacteriochlorophyll a , c and d -containing members with gas vesicles.…”

Section: Introductionmentioning

confidence: 99%

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Cryo-Electron Tomography Reveals the Complex Ultrastructural Organization of Multicellular Filamentous Chloroflexota (Chloroflexi) Bacteria

et al. 2020

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The cell biology of Chloroflexota is poorly studied. We applied cryo-focused ion beam milling and cryo-electron tomography to study the ultrastructural organization of thermophilic Roseiflexus castenholzii and Chloroflexus aggregans, and mesophilic "Ca. Viridilinea mediisalina." These species represent the three main lineages within a group of multicellular filamentous anoxygenic phototrophic Chloroflexota bacteria belonging to the Chloroflexales order. We found surprising structural complexity in the Chloroflexales. As with filamentous cyanobacteria, cells of C. aggregans and "Ca. Viridilinea mediisalina" share the outer membrane-like layers of their intricate multilayer cell envelope. Additionally, cells of R. castenholzii and "Ca. Viridilinea mediisalina" are connected by septal channels that resemble cyanobacterial septal junctions. All three strains possess long pili anchored close to cell-to-cell junctions, a morphological feature comparable to that observed in cyanobacteria. The cytoplasm of the Chloroflexales bacteria is crowded with intracellular organelles such as different types of storage granules, membrane vesicles, chlorosomes, gas vesicles, chemoreceptor-like arrays, and cytoplasmic filaments. We observed a higher level of complexity in the mesophilic strain compared to the thermophilic strains with regards to the composition of intracellular bodies and the organization of the cell envelope. The ultrastructural details that we describe in these Chloroflexales bacteria will motivate further cell biological studies, given that the function and evolution of the many discovered morphological traits remain enigmatic in this diverse and widespread bacterial group.

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Section: Chlorosomesmentioning

confidence: 89%

Section: Gas Vesiclesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cryo-Electron Tomography Reveals the Complex Ultrastructural Organization of Multicellular Filamentous Chloroflexota (Chloroflexi) Bacteria

et al. 2020

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“…Over multiple rounds of optimization, the growth medium was adjusted to contain 2 mM ferrous chloride and 1.2 mM sodium acetate and to be a 1:1 dilution of the original freshwater medium, kept at a pH of ~7.5. In addition, cultures were generally incubated in soft agar (0.2-0.3%; w/v) 38 to promote faster growth of 'Ca. Chx.…”

Section: Enrichment Cultivation Of 'Ca Chx Allophototropha'mentioning

confidence: 99%

Anoxygenic phototrophicChloroflexotamember uses a Type I reaction center

Tsuji

Shaw

Nagashima

et al. 2020

Preprint

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SummaryChlorophyll-based phototrophy is performed using quinone and/or Fe-S type reaction centers1,2. Unlike oxygenic phototrophs, where both reaction center classes are used in tandem as Photosystem II and Photosystem I, anoxygenic phototrophs use only one class of reaction center, termed Type II (RCII) or Type I (RCI) reaction centers, separately for phototrophy3. Here we report the cultivation and characterization of a filamentous anoxygenic phototroph within the Chloroflexota (formerly Chloroflexi4) phylum, provisionally named ‘Candidatus Chlorohelix allophototropha’, that performs phototrophy using a distinct fourth clade of RCI protein, despite placing sister taxonomically to RCII-utilizing Chloroflexota members. ‘Ca. Chx. allophototropha’ contains chlorosomes, uses bacteriochlorophyll c, and encodes the FMO protein like other RCI-utilizing phototrophs in the Chlorobiales and Chloracidobacterales orders5. ‘Ca. Chx. allophototropha’ also encodes the potential for carbon fixation using the Calvin-Benson-Bassham (CBB) cycle, unlike all known RCI-utilizing phototrophs6. The discovery of ‘Ca. Chx. allophototropha’, as the first representative of a novel Chloroflexota order (i.e., ‘Ca. Chloroheliales’), sheds light on longstanding questions about the evolution of photosynthesis, including the origin of chlorosomes among RCII-utilizing Chloroflexota members5,7. The Chloroflexota is now the only phylum outside the Cyanobacteria containing genomic potential for both quinone and Fe-S type reaction centers and can thus serve as an additional system for exploring fundamental questions about the evolution of photosynthesis.

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Biodiversity and taxonomy

2024

Anoxygenic Phototrophic Bacteria

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‘Candidatus Viridilinea mediisalina’, a novel phototrophic Chloroflexi bacterium from a Siberian soda lake

Cited by 18 publications

References 30 publications

Cryo-Electron Tomography Reveals the Complex Ultrastructural Organization of Multicellular Filamentous Chloroflexota (Chloroflexi) Bacteria

Cryo-Electron Tomography Reveals the Complex Ultrastructural Organization of Multicellular Filamentous Chloroflexota (Chloroflexi) Bacteria

Anoxygenic phototrophicChloroflexotamember uses a Type I reaction center

Biodiversity and taxonomy

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