‘Candidatus phytoplasma solani’ genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors

Mitrović, Milana; Jakovljević, Miljana; Jović, Jelena; Krstić, Oliver; Kosovac, Andrea; Trivellone, Valeria; Jermini, Mauro; Toševski, Ivo; Cvrković, Tatjana

doi:10.1007/s10658-015-0800-y

Cited by 33 publications

(41 citation statements)

References 23 publications

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“…"Ca. P. solani", which is the cause of quarantine, is infectious in economically value crops such as tomato, grapevine, pepper, eggplant, corn, potato, and beet (Jovic et al, 2009;Belli et al, 2010;Quaglino et al, 2013Amaral-Mello et al, 2006Mitrovic et al, 2016;Ember et al, 2011;Del Serrone et al, 2001;Vellios andLioliopoulou, 2007, Azza andMohamed Eman, 2016).…”

Section: Discussionmentioning

confidence: 99%

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Güller¹,

Usta²

2020

Türk Tarım Ve Doğa Bilimleri Dergisi

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Phytoplasma-type symptoms were noted in tomato (Lycopersicum esculentum L.) in Bingöl province of Turkey. The remarkable symptoms include witch's broom, rosetting, purple curled and crispy leaves, small leaves in the upper branches and excessively elongated calyx. Genomic DNA isolation was performed to identify possible pathogens from the leaves of 11 plants with and without symptom. In the Nested-PCR test performed using universal primer sets, DNA bands of approximately 1200 bp size were obtained in 4 of 11 samples. Randomly chosen two DNA bands primed R16F2n/R16R2 were cloned into an appropriate plasmid vector to further characterizations. The recombinant plasmid DNAs purified were sequenced in both directions. Molecular assays of the 16S rRNA sequence confirmed the existence of the "Canditatus Phytoplasma solani" (16SrXII-A group) (similarity coefficient 1.00) (Accession no: MT279680) and the "Canditatus Phytoplasma trifolii" (16SrVI-A group) (similarity coefficient 1.00) (Accession no: MT279852) in the infected tomato samples. The isolates associated with tomato-phytoplasma were named as 'Bingöl D11' and 'Bingöl D90' isolates, respectively. The phylogenetic dendrogram created also confirmed where both pathogens belong. This current paper is documented in the first record of "Ca. P. solani" (16SrXII-A) and "Ca. P. trifolii" (16SrVI-A) in naturally diseased tomato in Bingöl of Turkey.

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Section: Discussionmentioning

confidence: 99%

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Güller¹,

Usta²

2020

Türk Tarım Ve Doğa Bilimleri Dergisi

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“…P. solani’ stamp genotypes identified at the Kladovo location (Rqg50, GGY and Rpm35) also contribute to this assumption by indicating possible multiple vectors due to the known occurrence of these genotypes in vectors of other ‘ Ca. P. solani’ pathosystems [ 6 , 20 , 23 , 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

“…P. solani’ and ‘ Ca. P. asteris’ isolates [ 6 , 23 , 39 , 40 , 42 , 44 ] and was applied to all C. arvensis samples from this study. This also included all non-symptomatic bindweed isolates negative for any phytoplasmas as determined by 16S rRNA analysis, which were used as negative controls.…”

Section: Methodsmentioning

confidence: 99%

“…P. solani’ and ‘ Ca. P. asteris’ isolates from previous studies [ 6 , 23 , 39 , 40 , 42 , 44 ]; B: negative control containing PCR mixture and molecular grade water; M: DNA ladder 100 bp (Serva).…”

Section: Figurementioning

confidence: 99%

“…Given the epidemiological and ecological complexity of ‘ Ca. P. solani’-induced diseases [ 6 , 7 , 8 , 19 , 20 , 21 , 22 , 23 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 ] and its evolutionary relatedness to ‘ Ca. P. convolvuli’, the focus of this study was to analyze the (co)occurrence of the two pathogens in shared host plant and related symptoms.…”

Section: Introductionmentioning

confidence: 99%

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Symptomatology, (Co)occurrence and Differential Diagnostic PCR Identification of ‘Ca. Phytoplasma solani’ and ‘Ca. Phytoplasma convolvuli’ in Field Bindweed

et al. 2021

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Field bindweed (Convolvulus arvensis) is one of the major natural plant hosts and reservoirs of ‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’), the causal agent of plant diseases in diverse agricultural crops, including Bois noir (BN) disease of grapevine. Phylogenetically, the most closely related phytoplasma to ‘Ca. P. solani’, the ‘Ca. P. convolvuli’, induces disease in field bindweed that is known by its symptoms as bindweed yellows (BY). The occurrence, coinfection and symptoms association of the two phytoplasmas in shared host plants were the subject of this study. Specific primers for the amplification of the elongation factor Tu gene (tuf) were developed for the identification of ‘Ca. P. convolvuli’ (by conventional nested PCR), as well as primers for simultaneous detection of ‘Ca. P. solani’ and ‘Ca. P. convolvuli’ by duplex SYBR Green real-time PCR. Among symptomatic bindweed plants, 25 and 41% were infected with a single phytoplasma species, ‘Ca. P. solani’ and ‘Ca. P. convolvuli’, respectively, while 34% were infected with both phytoplasmas. None of the non-symptomatic control plants carried phytoplasma, while non-symptomatic plants from our previous epidemiological studies in BN-affected vineyards were confirmed to be infected solely with ‘Ca. P. solani’. Stamp gene typing revealed Rqg50 and Rqg31 ‘Ca. P. solani’ genotypes in plants coinfected with ‘Ca. P. convolvuli’, while three diverse genotypes (Rqg50, GGY and Rpm35) were identified in a single locality with symptomatic bindweeds infected solely with ‘Ca. P. solani’. Variations in symptoms and their association with each of the phytoplasmas are described and documented. The symptom of bushy appearance could be single out as specific for ‘Ca. P. convolvuli’ infection, while occurrence of ‘Ca. P. solani’ could not be unequivocally associated with specific alterations in infected bindweeds. The results are discussed in the context of the epidemiological and ecological complexity of ‘Ca. P. solani’-induced diseases and the relationship between the two phytoplasma relatives in shared host plant.

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Phytoplasmas Infecting Vegetable, Pulse and Oil Crops

Martini

Delić

Liefting³

et al. 2018

Phytoplasmas: Plant Pathogenic Bacteria - I

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‘Candidatus phytoplasma solani’ genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors

Cited by 33 publications

References 23 publications

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Symptomatology, (Co)occurrence and Differential Diagnostic PCR Identification of ‘Ca. Phytoplasma solani’ and ‘Ca. Phytoplasma convolvuli’ in Field Bindweed

Phytoplasmas Infecting Vegetable, Pulse and Oil Crops

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