'Candidatus Odyssella thessalonicensis' gen. nov., sp. nov., an obligate intracellular parasite of Acanthamoeba species.

Birtles, Richard J.; Rowbotham, T. J.; Michel, R.; Pitcher, D.G.; Lascola, Bernard; Alexiou-Daniel, S.; Raoult, Didier

doi:10.1099/00207713-50-1-63

Cited by 79 publications

(76 citation statements)

References 34 publications

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“…The protozoan endosymbionts Holospora obtusa and 'Candidatus Odyssella thessalonicensis' (Birtles et al, 2000) formed a cluster with low bootstrap values in our study. The two strains also formed distinct lineages with a deep branch.…”

Section: The Cluster Of the Order Rickettsialesmentioning

confidence: 94%

The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov.

Lee

Liu

Anzai

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

263

118

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Section: The Cluster Of the Order Rickettsialesmentioning

confidence: 94%

The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov.

Lee

Liu

Anzai

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

263

118

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“…UV-7 from activated-sludge samples by co-cultivation with amoebae Free-living amoebae are known as natural hosts of environmental chlamydiae Fritsche et al, 2000;Horn et al, 2000) and of a variety of phylogenetically diverse obligate intracellular bacterial symbionts (Birtles et al, 2000;Fritsche et al, 1999;Horn et al, , 2002. In addition, they serve as vehicles for dissemination of different bacterial pathogens such as Legionella pneumophila, Francisella tularensis and Escherichia coli O157 (Abd et al, 2003;Barker et al, 1999;Goebel & Gross, 2001;Harb et al, 2000).…”

Section: Resultsmentioning

confidence: 99%

Recovery of an environmental chlamydia strain from activated sludge by co-cultivation with Acanthamoeba sp.

Collingro

Poppert²,

Heinz

et al. 2005

Microbiology

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Chlamydiae are a unique group of obligate intracellular bacteria comprising important pathogens of vertebrates as well as symbionts of free-living amoebae. Although there is ample molecular evidence for a huge diversity and wide distribution of chlamydiae in nature, environmental chlamydiae are currently represented by only few isolates. This paper reports the recovery of a novel environmental chlamydia strain from activated sludge by co-cultivation with Acanthamoeba sp. The recovered environmental chlamydia strain UV-7 showed the characteristic morphology of chlamydial developmental stages as revealed by electron microscopy and was identified as a new member of the family Parachlamydiaceae (98·7 % 16S rRNA sequence similarity to Parachlamydia acanthamoebae). Infection studies suggested that Parachlamydia sp. UV-7 is not confined to amoeba hosts but is also able to invade mammalian cells. These findings outline a new straightforward approach to retrieving environmental chlamydiae from nature without prior, tedious isolation and cultivation of their natural host cells, and lend further support to suggested implications of environmental chlamydiae for public health.

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“…Until recently, the ability to monitor the microbial community structure was limited by the lack of a suitable cultivation strategy to define the composition of species, and the relative abundance of specific populations, in microbial communities (Crawford, 2005). The method of amoebal coculture has been used to discover many previously unknown micro-organisms (Adekambi et al, 2004;Adeleke et al, 2001;Birtles et al, 2000;La Scola et al, 2000, 2004a, as well as to observe a new niche for survival of pathogenic microorganisms in water (Abd et al, 2005;Greub & Raoult, 2002;Landers et al, 2000). We use the term amoeba-resistant bacteria (ARB) instead of endosymbionts, since many of the bacteria able to resist destruction by free-living amoebae do not represent true endosymbionts, and it is also the case that endosymbionts may be endosymbiotic or lytic in a given amoeba, depending on the environmental conditions.…”

Section: Introductionmentioning

confidence: 99%

Amoeba co-culture of soil specimens recovered 33 different bacteria, including four new species and Streptococcus pneumoniae

et al. 2009

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Amoeba-resistant bacteria (ARB), such as Legionella spp., are currently regarded as potential human pathogens that live in the natural environment, and thus their habitat is regarded as a reservoir of human pathogens. To detect ARB in human and environmental samples, co-culture with amoebae has been demonstrated to be an efficient tool. However, to date, only water samples from cooling towers and hospital water supplies have been investigated as possible reservoirs of ARB using this procedure. In the present study, we studied the ARB population of 11 diverse soil and sand sources in proximity to human environments; these sources included the university, the station, hospitals, the square, parks and public beaches in the city of Marseilles, France. As a result, a total of 33 different species of ARB were identified. The ability to grow within and/or lyse amoebae was demonstrated, for what is believed to be the first time, for several species; moreover, 20 of the isolates (61 %), including Streptococcus pneumoniae, have been described as human pathogens. However, Legionella spp. were not isolated. Four isolates are likely to be the members of new or uncharacterized genera or species, and their capability to be human pathogens needs to be determined. This preliminary work demonstrates that soils and sands in the vicinity of humans are reservoirs of human pathogenic ARB.

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'Candidatus Odyssella thessalonicensis' gen. nov., sp. nov., an obligate intracellular parasite of Acanthamoeba species.

Cited by 79 publications

References 34 publications

The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov.

The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov.

Recovery of an environmental chlamydia strain from activated sludge by co-cultivation with Acanthamoeba sp.

Amoeba co-culture of soil specimens recovered 33 different bacteria, including four new species and Streptococcus pneumoniae

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