Candidate Resistance Gene Discovery: Resistance Gene Analog Characterisation and Differential Gene Expression Analysis in Musa-Mycosphaerella Host-Pathogen Interactions

Miller, Robert N.; Passos, M.A.N.; Emediato, F.L.; Teixeira, Cristiane de Camargo; Júnior, G. J. Pappas

doi:10.17660/actahortic.2011.897.19

Cited by 6 publications

(5 citation statements)

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“…oryzae (Song et al 1995). In a previous study in M. acuminata (Miller et al 2011), sequence similarity analysis of amplification products generated using degenerate primers for RLKs identified numerous sequences with significant similarity to R -gene and RGA sequences for this class. A total of 20 serine/threonine protein kinases were also identified on the basis of KOG function assignment.…”

Section: Discussionmentioning

confidence: 93%

Development of expressed sequence tag and expressed sequence tag–simple sequence repeat marker resources for Musa acuminata

et al. 2012

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Section: Discussionmentioning

confidence: 93%

Development of expressed sequence tag and expressed sequence tag–simple sequence repeat marker resources for Musa acuminata

et al. 2012

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“…Previous studies have used the predicted protein structures and locations in cells to classify R genes into five groups: nucleotide binding site -leucine-rich repeat (NBS-LRR), in-tracellular serine -threonine protein kinase (PK), leucine-rich repeat -single transmembrane region (LRR-TM), PK-LRR-TM, and signaling anchor -coiled-coil domain (SA-CC) (Hammond-Kosack and Jones 1997;Meyers et al 1999;Jones 2001). Conserved motifs or hydrophobic sites at the NBS have enabled a novel polymerase chain reaction (PCR)-based approach that uses degenerate primers to amplify resistance gene analogs (RGA), as putative or tentative disease resistance genes, from many plant species (Kanazin et al 1996;Leister et al 1996;Spielmeyer et al 1998;Yan et al 2003Yan et al , 2004Miller et al 2011;Selvaraj et al 2011). Genetic analyses have associated many RGAs to known R genes that confer resistance to a number of diseases (Huang and Gill 2001;Madsen et al 2003;Mutlu et al 2006Mutlu et al , 2008Naik et al 2006;Shang et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Application of resistance gene analog markers to analyses of genetic structure and diversity in rice

Ren

Gao

et al. 2013

Genome

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Plant disease resistance gene analog (RGA) markers were designed according to the conserved sequence of known RGAs and used to map resistance genes. We used genome-wide RGA markers for genetic analyses of structure and diversity in a global rice germplasm collection. Of the 472 RGA markers, 138 were polymorphic and these were applied to 178 entries selected from the USDA rice core collection. Results from the RGA markers were similar between two methods, UPGMA and STRUCTURE. Additionally, the results from RGA markers in our study were agreeable with those previously reported from SSR markers, including cluster of ancestral classification, genetic diversity estimates, genetic relatedness, and cluster of geographic origins. These results suggest that RGA markers are applicable for analyses of genetic structure and diversity in rice. However, unlike SSR markers, the RGA markers failed to differentiate temperate japonica, tropical japonica, and aromatic subgroups. The restricted way for developing RGA markers from the cDNA sequence might limit the polymorphism of RGA markers in the genome, thus limiting the discriminatory power in comparison with SSR markers. Genetic differentiation obtained using RGA markers may be useful for defining genetic diversity of a suite of random R genes in plants, as many studies show a differentiation of resistance to a wide array of pathogens. They could also help to characterize the genetic structure and geographic distribution in crops, including rice, wheat, barley, and banana.

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“…The cultivated bananas are vegetatively propagated and the vegetative propagation leads to genetic erosion, owing to the limited availability of the gene pool, and plants become more prone to diseases, pests, and ecological changes (Miller et al 2011;Perrier et al 2011). A recent survey on banana diversity establishes the fact that wild species carry the traits critical to save bananas from climate change, pests, and diseases (Kallow et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Harnessing stress-tolerant wild bananas for crop improvement

Panwar

Chaudhry

Kumar

et al. 2022

Crop & Pasture Science

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Climate change impacts crop production through the imposition of different abiotic and biotic stresses, and by altering the prevalence of pests and diseases. The wild relatives of crop plants exhibit enhanced tolerance to environmental stresses due to reduced severity of selection of agriculturally important traits. Wild bananas represent a largely untapped wealth of genetic diversity. Although some wild relatives of the banana crop have been screened for their tolerance to different biotic and abiotic stresses, many remain to be explored. The wild bananas show more hardiness and higher resilience to different stressors in comparison to their cultivated counterparts. They have been harnessed in banana improvement programmes to enhance stress tolerance and productivity. To utilise wild bananas for crop improvement, they need to be readily available to breeders. This warrants devising conservation strategies and the development and maintenance of centres from which different accessions can be procured. In this article, we have discussed some important biotic and abiotic stresses including banana wilt disease, Black Sigatoka disease, viral diseases, salt stress and drought stress where wild bananas are used for imparting tolerance. The conservation of wild bananas, related challenges and contemporary limitations related to their use for crop improvement has also been outlined. Bananas being most important food crop in the world and generally understudied, here, we present a comprehensive review of the use of wild relatives of banana and their related germplasm for the improvement of biotic and abiotic stress tolerance.

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Candidate Resistance Gene Discovery: Resistance Gene Analog Characterisation and Differential Gene Expression Analysis in Musa-Mycosphaerella Host-Pathogen Interactions

Cited by 6 publications

References 0 publications

Development of expressed sequence tag and expressed sequence tag–simple sequence repeat marker resources for Musa acuminata

Development of expressed sequence tag and expressed sequence tag–simple sequence repeat marker resources for Musa acuminata

Application of resistance gene analog markers to analyses of genetic structure and diversity in rice

Harnessing stress-tolerant wild bananas for crop improvement

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