Candidate nematicidal proteins in a new &lt;i&gt;Pseudomonas veronii&lt;/i&gt; isolate identified by its antagonistic properties against &lt;i&gt;Xiphinema index&lt;/i&gt;

Canchignia, Hayron; Altimira, Fabiola; Montes, Christian; Sanchez, Evelyn Aparecida Mecenero; Tapia, Eduardo; Miccono, María; Espinoza, Daniel; Aguirre, Carlos; Seeger, Michael; Prieto, Humberto

doi:10.2323/jgam.2016.07.001

Cited by 10 publications

(12 citation statements)

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“…cubense by 87.4% (Nel et al 2006). In addition, in diverse crop and tree species, such as radish (Raphanus sativus), grapevine (Vitis vinifera), and eucalyptus (Eucalyptus urophylla), WCS417 effectively controlled plant diseases (Canchignia et al 2017;Leeman et al 1996a;Ran et al 2005). The spectrum of effectiveness of the biological control activity seems to be relatively unspecific, as besides the soil-borne fungal diseases mentioned above, WCS417 was demonstrated to control other soil-borne diseases, such as bacterial wilt, caused by Ralstonia solanacearum (Ran et al 2005) and the nematode Xiphinema index (Canchignia et al 2017).…”

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“…In addition, in diverse crop and tree species, such as radish (Raphanus sativus), grapevine (Vitis vinifera), and eucalyptus (Eucalyptus urophylla), WCS417 effectively controlled plant diseases (Canchignia et al 2017;Leeman et al 1996a;Ran et al 2005). The spectrum of effectiveness of the biological control activity seems to be relatively unspecific, as besides the soil-borne fungal diseases mentioned above, WCS417 was demonstrated to control other soil-borne diseases, such as bacterial wilt, caused by Ralstonia solanacearum (Ran et al 2005) and the nematode Xiphinema index (Canchignia et al 2017). In a recent study, colonization of the roots of peppermint (Mentha piperita) by WCS417 improved the antioxidant status of plants grown under drought stress leading to enhanced drought tolerance (Chiappero et al 2019), and in the model plant Arabidopsis (Arabidopsis thaliana) it improved salt tolerance (Loo et al 2020).…”

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Pseudomonas simiae WCS417: star track of a model beneficial rhizobacterium

et al. 2020

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Background Since the 1980s, numerous mutualistic Pseudomonas spp. strains have been used in studies on the biology of plant growth-promoting rhizobacteria (PGPR) and their interactions with host plants. In 1988, a strain from the Pseudomonas fluorescens group, WCS417, was isolated from lesions of wheat roots growing in a take-all disease-suppressive soil. In subsequent trials, WCS417 limited the build-up of take-all disease in field-grown wheat and significantly increased wheat yield. In 1991, WCS417 was featured in one of the first landmark studies on rhizobacteria-induced systemic resistance (ISR), in which it was shown to confer systemic immunity in carnation (Dianthus caryophyllus) against Fusarium wilt. The discovery that WCS417 conferred systemic immunity in the model plant species Arabidopsis thaliana in 1996 incited intensive research on the molecular mechanisms by which PGPR promote plant growth and induce broad-spectrum disease resistance in plants. Since then, the strain name appeared in over 750 studies on beneficial plant-microbe interactions. Scope In this review, we will highlight key discoveries in plant-microbe interactions research that have emerged from over 30 years of research featuring WCS417 as a model rhizobacterial strain. WCS417 was instrumental in improving our understanding of the microbial determinants that are involved in root colonization and the establishment of mutually beneficial interactions with the host plant. The model strain also provided novel insight into the molecular mechanisms of plant growth promotion and the onset and expression of rhizobacteria-ISR. More recently, WCS417 has been featured in studies on host immune evasion during root colonization, and chemical communication in the rhizosphere during root microbiome assembly. Conclusions Numerous studies on the modes of action of WCS417 have provided major conceptual advances in our understanding of how free-living mutualists colonize the rhizosphere, modulate plant immunity, and promote plant growth. The concepts may prove useful in our understanding of the molecular mechanisms involved in other binary plant-beneficial microbe interactions, and in more complex microbial community contexts, such as the root microbiome.

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Pseudomonas simiae WCS417: star track of a model beneficial rhizobacterium

et al. 2020

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“…Pseudomonas strains have been isolated from each of the ecological niches within plants including roots (rhizosphere), leaves (phyllosphere), and tissues (endosphere) [108,109]. In previous studies, Pseudomonas strains isolated from Chilean agricultural soils with nematicidal and herbicide-degrading activities have been characterized [110,111]. On the other hand, the Stenotrophomonas genus has been associated with soil and plants [112][113][114], showing beneficial effects on plant growth and health [86].…”

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confidence: 99%

Microbial Diversity of Psychrotolerant Bacteria Isolated from Wild Flora of Andes Mountains and Patagonia of Chile towards the Selection of Plant Growth-Promoting Bacterial Consortia to Alleviate Cold Stress in Plants

et al. 2021

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Cold stress decreases the growth and productivity of agricultural crops. Psychrotolerant plant growth-promoting bacteria (PGPB) may protect and promote plant growth at low temperatures. The aims of this study were to isolate and characterize psychrotolerant PGPB from wild flora of Andes Mountains and Patagonia of Chile and to formulate PGPB consortia. Psychrotolerant strains were isolated from 11 wild plants (rhizosphere and phyllosphere) during winter of 2015. For the first time, bacteria associated with Calycera, Orites, and Chusquea plant genera were reported. More than 50% of the 130 isolates showed ≥33% bacterial cell survival at temperatures below zero. Seventy strains of Pseudomonas, Curtobacterium, Janthinobacterium, Stenotrophomonas, Serratia, Brevundimonas, Xanthomonas, Frondihabitans, Arthrobacter, Pseudarthrobacter, Paenarthrobacter, Brachybacterium, Clavibacter, Sporosarcina, Bacillus, Solibacillus, Flavobacterium, and Pedobacter genera were identified by 16S rRNA gene sequence analyses. Ten strains were selected based on psychrotolerance, auxin production, phosphate solubilization, presence of nifH (nitrogenase reductase) and acdS (1-aminocyclopropane-1-carboxylate (ACC) deaminase) genes, and anti-phytopathogenic activities. Two of the three bacterial consortia formulated promoted tomato plant growth under normal and cold stress conditions. The bacterial consortium composed of Pseudomonas sp. TmR5a & Curtobacterium sp. BmP22c that possesses ACC deaminase and ice recrystallization inhibition activities is a promising candidate for future cold stress studies.

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“…To allow protease activity, the gel was incubated in the presence of a buffer with co-factors (6.055 g/L Tris-HCl, 11.69 g/L NaCl, 0.07 g/L ZnCl 2 , 0.74 g/L CaCl 2 ·2H 2 O, and 0.2 g/L NaN 3 , pH 7.4) for 12 h at 25 °C. For detection, the gel was stained with Coomassie blue for 1 h. Proteolytic activity was assessed by visualization of a transparent area on the blue background of the stained gel [ 21 , 22 ].…”

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confidence: 99%

Identification of Exoenzymes Secreted by Entomopathogenic Fungus Beauveria pseudobassiana RGM 2184 and Their Effect on the Degradation of Cocoons and Pupae of Quarantine Pest Lobesia botrana

Arias-Aravena

Altimira

Gutiérrez

et al. 2022

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Beauveria pseudobassiana RGM 2184 has shown 80% maximum efficacy against the pest Lobesia botrana in the autumn and winter seasons. This suggests that the strain possesses an interesting battery of enzymes that are cold-adapted to penetrate the thick and hydrophobic cocoon of L. botrana. In this study, screening of the proteolytic, lipolytic, and chitinolytic activity of enzyme extracts secreted by the RGM 2184 strain was carried out in various culture media. The enzyme extracts with the highest activity were subjected to zymography and mass spectrometry. These analyses allowed the identification of two proteases, two lipases, and three chitinases. Comparative analysis indicated that the degree of similarity between these enzymes was substantially reduced when the highest degree of taxonomic relatedness between RGM 2184 and the entomopathogenic fungus strain was at the family level. These results suggest that there is a wide variety of exoenzymes in entomopathogenic fungi species belonging to the order Hypocreales. On the other hand, exoenzyme extract exposure of cocoons and pupae of L. botrana provoked damage at 10 °C. Additionally, an analysis of the amino acid composition of the RGM 2184 exoenzyme grouped them close to the cold-adapted protein cluster. These results support the use of this strain to control pests in autumn and winter. Additionally, these antecedents can form a scaffold for the future characterization of these exoenzymes along with the optimization of the strain’s biocontrol ability by overexpressing them.

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Candidate nematicidal proteins in a new <i>Pseudomonas veronii</i> isolate identified by its antagonistic properties against <i>Xiphinema index</i>

Cited by 10 publications

References 55 publications

Pseudomonas simiae WCS417: star track of a model beneficial rhizobacterium

Pseudomonas simiae WCS417: star track of a model beneficial rhizobacterium

Microbial Diversity of Psychrotolerant Bacteria Isolated from Wild Flora of Andes Mountains and Patagonia of Chile towards the Selection of Plant Growth-Promoting Bacterial Consortia to Alleviate Cold Stress in Plants

Identification of Exoenzymes Secreted by Entomopathogenic Fungus Beauveria pseudobassiana RGM 2184 and Their Effect on the Degradation of Cocoons and Pupae of Quarantine Pest Lobesia botrana

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