Candidate markers for stem and early progenitor cells, Musashi‐1 and Hes1, are expressed in crypt base columnar cells of mouse small intestine

Kayahara, Takahisa; Sawada, Mitsutaka; Takaishi, Shigeo; Fukui, Hirokazu; Seno, Hiroshi; Fukuzawa, Hiroaki; Suzuki, Kazuyuki; Hayashi, Hiroshi; Kageyama, Ryoichiro; Okano, Hideyuki; Chiba, Tsutomu

doi:10.1016/s0014-5793(02)03896-6

Cited by 281 publications

(253 citation statements)

References 27 publications

(45 reference statements)

Supporting

Mentioning

230

Contrasting

Unclassified

Order By: Relevance

“…2C) and Musashi-1 (Msi-1; Fig. 2D; IshizuyaOka et al, 2003), both of which have been proposed as candidate markers for mammalian intestinal epithelial stem cells and their immediate descendants (Kayahara et al, 2003;Potten et al, 2003;Okano et al, 2005;He et al, 2004). These immunohistochemical studies reinforce our hypothesis that the adult epithelial primordia of the X. laevis intestine include stem cells analogous to mammalian intestinal stem cells, suggesting the existence of conserved regulatory mechanisms.…”

Section: Amphibian Intestinal Stem Cells Are Analogous To the Mammalisupporting

confidence: 80%

Regulation of adult intestinal epithelial stem cell development by thyroid hormone during Xenopus laevis metamorphosis

Ishizuya‐Oka

Shi

2007

Developmental Dynamics

View full text Add to dashboard Cite

During amphibian metamorphosis, most or all of the larval intestinal epithelial cells undergo apoptosis. In contrast, stem cells of yet-unknown origin actively proliferate and, under the influence of the connective tissue, differentiate into the adult epithelium analogous to the mammalian counterpart. Thus, amphibian intestinal remodeling is useful for studying the stem cell niche, the clarification of which is urgently needed for regenerative therapies. This review highlights the molecular aspects of the niche using the Xenopus laevis intestine as a model. Because amphibian metamorphosis is completely controlled by thyroid hormone (TH), the analysis of TH response genes serves as a powerful means for clarifying its molecular mechanisms. Although functional analysis of the genes is still on the way, recent progresses in organ culture and transgenic studies have gradually uncovered important roles of cell-cell and cell-extracellular matrix interactions through stromelysin-3 and sonic hedgehog/bone morphogenetic protein-4 signaling pathway in the epithelial stem cell development. Developmental Dynamics 236:3358 -3368, 2007.

show abstract

Section: Amphibian Intestinal Stem Cells Are Analogous To the Mammalisupporting

confidence: 80%

Regulation of adult intestinal epithelial stem cell development by thyroid hormone during Xenopus laevis metamorphosis

Ishizuya‐Oka

Shi

2007

Developmental Dynamics

View full text Add to dashboard Cite

show abstract

“…Musashi-1-positive intestinal crypt cells are cells that have many characteristics of stem cells (Kayahara et al, 2003;Potten et al, 2003). The musashi-1 immunohistochemistry, however, did not show any differences in the number of musashi-1-positive cells in Reg þ / þ and in Reg À/À mice (data not shown).…”

Section: Resultsmentioning

confidence: 86%

Reg I-knockout mice reveal its role in regulation of cell growth that is required in generation and maintenance of the villous structure of small intestine

et al. 2006

View full text Add to dashboard Cite

Reg I (regenerating gene product I) is a growth factor that plays a central role in the generation and regeneration of the gastric mucosal architecture. On the other hand, mouse Reg I mRNA is expressed at the highest levels in the small intestine among the gastrointestinal tissues. In the current study, with the aim to clarify the role of Reg I protein in the small intestine, the temporal and spatial pattern of Reg I expression and the phenotype of Reg I-knockout mice in the tissue were examined. In the wild-type mice, immunohistochemistry localized Reg I protein expression in absorptive cells located in the lower half of the intestinal villi. Reg I expression was undetectable until embryonic day 13 (E13), when the fetal intestine still lacks villous structure; however, it dramatically increased at E17 along with the formation and maturation of the fetal intestinal villi. In the small intestine of the adult Reg I-knockout mice, less densely packed, round-shaped aberrant morphology of the absorptive cells was observed light microscopically, and electron microscopical examination revealed a strikingly loose connection of these cells to the basement membrane. Antiproliferating cell nuclear antigen staining and anti-Ki67 staining demonstrated the marked decrease in the number of proliferating cells in the small intestinal mucosa of the knockout mice. The cell migration speed visualized by one shot labeling of 5-bromodeoxyuridine was significantly slower in the knockout mice. These phenotypes of Reg I-knockout mice emerged, in accordance with the temporal pattern of Reg I expression described above, from E17. Reg I was considered to be a regulator of cell growth that is required to generate and maintain the villous structure of the small intestine.

show abstract

“…Msi-1 positively regulates the transcriptional repressor molecule, Hes-1 (Figure 7c). In the mouse small intestine, Msi-1 and Hes-1 are co-expressed in the cells just above the Paneth cell zone, although Hes-1 expression additionally extends to proliferative cells higher up the crypt [102]. Msi-1 also co-localizes with another putative stem cell marker, DCAMKL-1, at cell position 4 [103].…”

Section: Digestive Tractmentioning

confidence: 99%

Attributes of adult stem cells

Alison

Islam

2008

The Journal of Pathology

153

115

View full text Add to dashboard Cite

While cultured embryonic stem (ES) cells can be harvested in abundance and appear to be the most versatile of cells for regenerative medicine, adult stem cells also hold promise, but the identity and subsequent isolation of these comparatively rare cells remains problematic in most tissues, perhaps with the notable exception of the bone marrow. The ability to continuously self-renew and produce the differentiated progeny of the tissue of their location are their defining properties. Identifying surface molecules (markers) that would aid in stem cell isolation is a major goal. Considerable overlap exists between different putative organspecific stem cells in their repertoire of gene expression, often related to self-renewal, cell survival and cell adhesion. More robust tests of 'stemness' are now being employed, using lineage-specific genetic marking and tracking to show production of long-lived clones and multipotentiality in vivo. Moreover, the characterization of normal stem cells in specific tissues may provide a dividend for the treatment of cancer. The successful treatment of neoplastic disease may well require the specific targeting of neoplastic stem cells, cells that may well have many of the characteristics of their normal counterparts.

show abstract

Candidate markers for stem and early progenitor cells, Musashi‐1 and Hes1, are expressed in crypt base columnar cells of mouse small intestine

Cited by 281 publications

References 27 publications

Regulation of adult intestinal epithelial stem cell development by thyroid hormone during Xenopus laevis metamorphosis

Regulation of adult intestinal epithelial stem cell development by thyroid hormone during Xenopus laevis metamorphosis

Reg I-knockout mice reveal its role in regulation of cell growth that is required in generation and maintenance of the villous structure of small intestine

Attributes of adult stem cells

Contact Info

Product

Resources

About