Candidate chemosensory genes identified in the endoparasitoid Meteorus pulchricornis (Hymenoptera: Braconidae) by antennal transcriptome analysis

Sheng, Sheng; Liao, Cheng-Wu; Zheng, Yu; Zhou, Yu; Xu, Yan; Song, Wen‐Miao; He, Peng; Zhang, Jian; Wu, Fu‐An

doi:10.1016/j.cbd.2017.01.002

Cited by 42 publications

(46 citation statements)

References 68 publications

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“…However, some recent studies have demonstrated that several OBPs are specifically distributed in nonchemosensory organs, such as the male reproductive organs (Ban et al., ; Sun, Huang, Pelosi, & Wang, ). In the endoparasitoid wasp Meteorus pulchricornis , several OBP s ( MpulOBP6 , MpulOBP9 , MpulOBP10 , MpulOBP12 , MpulOBP15 , and MpulOBP16 ) are more highly expressed in the antennae, but MpulOBP2 and MpulOBP3 are predominantly expressed in the legs and abdomen, respectively (Sheng et al., ). Therefore, further functional analyses of these OBP s are needed to broaden our knowledge of olfaction.…”

Section: Introductionmentioning

confidence: 99%

Identification and expression profiles of novel odorant binding proteins and functional analysis of OBP99a in Bactrocera dorsalis

Zhang

Luo

et al. 2018

Arch Insect Biochem Physiol

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Odorant-binding proteins (OBPs) in insects are essential for mating and oviposition host selection. How these OBPs respond to different hosts at the mRNA level and their effects on behavior remain poorly characterized. The oriental fruit fly Bactrocera dorsalis is a highly invasive agricultural pest with an extremely broad host range and high fecundity. Based on our previously constructed B. dorsalis transcriptome, six OBPs that were differentially expressed during three different physiological adult stages were identified. A phylogenetic tree was constructed to illustrate the relationships of these six OBPs with OBP sequences from three other dipteran species (Drosophila melanogaster, Anopheles gambiae, and Ceratitis capitata).The spatiotemporal expression profiles of the six OBPs were analyzed using quantitative real-time PCR. Our results revealed that OBP19c, OBP44a, OBP99a, and OBP99d were abundantly expressed from the prepupa stage to the adult stage, and most of the OBPs were mainly expressed in the head, wings, and antennae. The expression levels of these OBPs were upregulated when female flies were exposed to their preferred hosts. Silencing OBP99a resulted fewer eggs being laid compared with the control group when the females were exposed to their preferred host, that is, banana, whereas more eggs were laid when a non-preferred host, that is, tomato, was used.Furthermore, silencing OBP99a led to sexually dimorphic mating behavior. dsOBP99a-injected males dramatically reduced courtship, whereas enhanced courtship was observed in the treated females.These data indicate that OBPs may participate in different biological processes of B. dorsalis. Our study will provide insight into the molecular mechanism of chemoreception and help develop ecologically friendly pest-control strategies.Arch. Insect Biochem. Physiol. 2018;98:e21452.wileyonlinelibrary.com/journal/arch

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Section: Introductionmentioning

confidence: 99%

Identification and expression profiles of novel odorant binding proteins and functional analysis of OBP99a in Bactrocera dorsalis

Zhang

Luo

et al. 2018

Arch Insect Biochem Physiol

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“…In terms of the number of olfactory proteins, C. obducta had similar amount OBPs compared with the whole body transcriptome of Oedaleus infernalis . Compared with the insect antenna transcriptome (Chang et al, 2017;Nie et al, 2017;Rojas et al, 2018;Sheng et al, 2017;Wu et al, 2017;Yang et al, 2017Yang et al, , 2018) (File S8), C. obducta had an intermediate number of OBPs. The number of olfactory proteins identified in the whole body transcriptome of C. obducta is smaller than that inmost other species based on analyses of insect antennal genes.…”

Section: Discussionmentioning

confidence: 99%

The whole body transcriptome of Coleophora obducta reveals important olfactory proteins

Wang

Tao

et al. 2020

PeerJ

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Background The tiny casebearer moth Coleophora obducta, an important defoliator of Larix spp., is a major threat to ecological security in north China. Studies have shown that C. obducta is strongly specific to host plants; it is unable complete its life cycle without Larix spp. The sex pheromones of C. obducta Z5-10:OH have been elucidated; and eight types of antennae sensilla, have been detected, indicating that an exploration of its olfactory proteins is necessary, due to the general lack of information on this topic. Methods We investigated the whole body transcriptome of C. obducta, performed a phylogenetic analysis of its olfactory proteins and produced expression profiles of three pheromone-binding proteins (CobdPBPs) by qRT–PCR. Results We identified 16 odorant binding proteins, 14 chemosensory proteins, three sensory neuron membrane proteins, six odorant degrading enzymes, five antennal esterases, 13 odorant receptors, seven ionotropic receptors and 10 gustatory receptors, including three PBPs and one odorant co-receptor. Additionally, three putative pheromone receptors, two bitter gustatory receptors and five functional ionotropic receptors were found by phylogenetic analysis. The expression profiles of three PBPs in males and females showed that all of them exhibited male-specific expression and two were expressed at significantly higher levels in males. These data provide a molecular foundation from which to explore the olfactory recognition process and may be useful in the development of a new integrated pest management strategy targeting olfactory recognition of C. obducta.

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“…The total number of CSPs found in different insect species varies widely (Pelosi et al, 2014), but the low number of CSPs reported here is in agreement with that reported for the scarab beetle Anomala corpulenta . Further studies, including other non-sensory tissues such as wings and pheromone glands, may increase the quantity of the reported CSPs (Sheng et al, 2017) in addition to studying the expression of CSPs in other developmental stages . However, it is important to note that all four CSPs reported here exhibited palpbiased expression.…”

Section: Discussionmentioning

confidence: 99%

Peer Review #2 of "Sex- and tissue-specific expression of odorant-binding proteins and chemosensory proteins in adults of the scarab beetle Hylamorpha elegans (Burmeister) (Coleoptera: Scarabaeidae) (v0.1)"

2019

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In this study, we addressed the sex-and tissue-specific expression patterns of odorantbinding proteins (OBPs) and chemosensory proteins (CSPs) in Hylamorpha elegans (Burmeister), an important native scarab beetle pest species from Chile. Similar to other members of its family, this scarab beetle exhibit habits that make difficult to control the pest by conventional methods. Hence, alternative ways to manage the pest populations based on chemical communication and signaling (such as disrupting mating or host finding process) are highly desirable. However, developing pest-control methods based on chemical communication requires to understand the molecular basis for pheromone recognition/chemical perception in this species. Thus, with the aim of discovering olfactionrelated genes, we obtained the first reference transcriptome assembly of H. elegans. We used different tissues of adult beetles from males and females: antennae and maxillary palps, which are well known for embedded sensory organs. Then, the expression of predicted odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) was analyzed by qRT-PCR. In total, 165 transcripts related to chemoperception were predicted. Of these, 16 OBPs, including one pheromone-binding protein (PBP), and four CSPs were successfully amplified by qRT-PCR. All of these genes were differentially expressed in the sensory tissues with respect to the tibial tissue that was used as a control. The single predicted PBP found was highly expressed in the antennal tissues, particularly in males, while several OBPs and one CSP showed male-biased expression patterns, suggesting that these proteins may participate in sexual recognition process. In addition, a single CSP was expressed at higher levels in female palps than in any other studied condition, suggesting that this CSP would participate in oviposition process. Finally, all four CSPs exhibited palp

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Candidate chemosensory genes identified in the endoparasitoid Meteorus pulchricornis (Hymenoptera: Braconidae) by antennal transcriptome analysis

Cited by 42 publications

References 68 publications

Identification and expression profiles of novel odorant binding proteins and functional analysis of OBP99a in Bactrocera dorsalis

Identification and expression profiles of novel odorant binding proteins and functional analysis of OBP99a in Bactrocera dorsalis

The whole body transcriptome of Coleophora obducta reveals important olfactory proteins

Peer Review #2 of "Sex- and tissue-specific expression of odorant-binding proteins and chemosensory proteins in adults of the scarab beetle Hylamorpha elegans (Burmeister) (Coleoptera: Scarabaeidae) (v0.1)"

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