Candida utilis and Cyberlindnera (Pichia) jadinii: yeast relatives with expanding applications

Abstract

The yeast Candida utilis is used as a food additive and as a host for heterologous gene expression to produce various metabolites and proteins. Reliable protocols for intracellular production of recombinant proteins are available for C. utilis and have now been expanded to secrete proteins into the growth medium or to achieve surface display by linkage to a cell wall protein. A recombinant C. utilis strain was recently shown to induce oral tolerance in a mouse model of multiple sclerosis suggesting future applications in autoimmune therapy. Whole genome sequencing of C. utilis and its presumed parent Cyberlindnera (Pichia) jadinii demonstrated different ploidy but high sequence identity, consistent with identical recombinant technologies for both yeasts. C. jadinii was recently described as an antagonist to the important human fungal pathogen Candida albicans suggesting its use as a probiotic agent. The review summarizes the status of recombinant protein production in C. utilis, as well as current and future biotechnological and medical applications of C. utilis and C. jadinii.

Cyberlindnera jadinii (teleomorph Candida utilis) candidaemia in a patient with aplastic anaemia: a case report

“…This report describes what is believed to be the first case of Cyberlindnera ( Pichia ) jadinii ( Candida utilis ) candidaemia in a patient with aplastic anaemia. Cyberlindnera jadinii is reported to be the teleomorphic parental species for Candida utilis , a non-pathogenic yeast used as a food additive [ 2 ]. Candida utilis is reported to been opportunistic pathogen and has been isolated from a few superficial clinical specimens taken from immunocompromised patients [ 3 ], newborns [ 4 ] and elderly adults [ 5 ].…”

Production of high‐purity galacto‐oligosaccharides by depleting glucose and lactose from galacto‐oligosaccharide syrup with yeasts

“…C. jadinii can be considered as a safe yeast because it has been approved as GRAS (generally recognized as safe) by the Food and Therefore, C. jadinii could be used safely for the production of glucose-free GOS; moreover, the yeast biomass can be considered as a valuable by-product and could be valorised as dried fodder yeast and for preparation of bioemulsifiers, cell wall mannans and glucans as well (Buerth, Tielker, & Ernst, 2016;Campos, Stamford, & Sarubbo, 2014). Depletion of monosaccharides and disaccharides by D. anomala DMB Da-VT from 10% GOS medium was slow and very limited ( Figure 6).…”

Biotechnological application of yeasts in food science: Starter cultures, probiotics and enzyme production

“…), Pichia jadinii (Buerth et al . ), Pichia kluyveri (Ogunremi et al . ), Pichia kudriavzevii (Ogunremi et al .…”