Candida theae sp. nov., a new anamorphic beverage-associated member of the Lodderomyces clade

(GTG)5 MSP-PCR Fingerprinting as a Technique for Discrimination of Wine Associated Yeasts?

“…On the other hand, the primer M13 was able to differentiate 16 strains of S. cerevisiae , although with different amplification conditions [32]. M13 or both M13 and (GTG) 5 primers are widely used for assessment of yeast communities [33], and description of new genus, species or genotypes within species [21], [34], although Libkind [28] suggested that the primer M13 is not able to separate fingerprinting profiles in a complex of closely related species because it amplifies more conserved regions of DNA. Thus, as our goal was to discriminate related and unrelated yeast species, both primers had similar discriminatory power with our subset of 16 isolates, and the primer (GTG) 5 grouped all the isolates of S. cerevisiae in the same cluster, we chose primer (GTG) 5 for further evaluation.…”

“…Among them, the Microsatellite/Minisatellite Primed (MSP)-PCR Fingerprinting technique has been widely applied in the literature using primers as (GAC) 5 , (GACA) 4 , (GTG) 5 and M13. For example, the primer (GTG) 5 was frequently used to discriminate species of the genus Saccharomyces [3]–[8], characterize strains of non- Saccharomyces species [9]–[12], analyze yeast diversity [13]–[20], and describe new yeast genus and species [21]–[24]. Most of these studies use MSP-PCR fingerprinting as a preliminary clustering step for the choice of representative strains to be sequenced.…”

An Overview of the Yeast Biodiversity in the Galápagos Islands and Other Ecuadorian Regions

“…As part of a study on traditional fermented beverages we were able to find this yeast species even in unexpected substrates such as cassava fermented beverages which are produced in Yasuní National Park, located in the deep Ecuadorian Amazonia. This millenary beverage is still being produced in the same traditional way by autochthonous tribes of Waorani people [28]. In the literature C. tropicalis is reported to be collected from clinical samples.…”

DNA barcoding revealed Nematodospora valgi gen. nov., sp. nov. and Candida cetoniae sp. nov. in the Lodderomyces clade

“…The clade includes an additional 30 anamorphic Candida species, among them the major human pathogens Candida albicans, Candida dubliniensis, Candida parapsilosis, Candida metapsilosis, Candida orthopsilosis, and Candida tropicalis (Lachance et al 2011a). Members of the clade were isolated from various sources, such as marine habitats, food, plants, and soil (Lachance et al 2011;Nitiyon et al 2011;Nakase et al 2009;Limtong et al 2012;Sipicszki 2013), soft drinks (Chang et al 2012), and human and animals (Weems 1991;Wingard 1995;Trofa et al 2008;Cooper 2011;Kim & Sudbery 2011;Lachance et al 2011a). Studies conducted in different geographic regions showed that insects frequently host yeasts belonging to the Lodderomyces clade, including the human pathogens (Nguyen et al 2007;Suh et al 2008;Ji et al 2009, Urbina 2012.…”