Candida rugosa lipase for the biodiesel production from renewable sources

Iuliano, Mariagrazia; Sarno, Maria; Pasquale, S. De; Ponticorvo, Eleonora

doi:10.1016/j.renene.2020.08.019

Cited by 29 publications

(15 citation statements)

References 37 publications

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“…Xie and Huang obtained a 92.8% yield of methyl soybean biodiesel with CRL immobilized on graphene oxide Fe 3 O 4 nanocomposite 29 . Iuliano et al 30 . reached a 98% yield after 48 h of reaction at 45 °C using CRL immobilized on modified Fe 2 O 4 nanoparticles to obtain methyl biodiesel.…”

Section: Resultsmentioning

confidence: 99%

“…Candida rugosa lipase (CRL) is an enzyme used in several biotransformation processes; 23 among them its use as a catalyst lipase to obtain biodiesel stands out. Candida rugosa lipase has been studied for its ability to catalyze the reaction of biodiesel, being used in various immobilizing supports and with different types of raw materials 24–30 …”

Section: Introductionmentioning

confidence: 99%

“…Candida rugosa lipase has been studied for its ability to catalyze the reaction of biodiesel, being used in various immobilizing supports and with different types of raw materials. [24][25][26][27][28][29][30] p-Nitrobenzyl cellulose xanthate (NBXCel) was shown to be an efficient support for enzyme immobilization for biodiesel production. 31 Thus, considering NBXCel as a cellulosederived support to immobilize lipases, and the efficiency of CRL in catalyzing the reaction to obtain biodiesel, this work studied the obtainment of soybean biodiesel, using methanol and ethanol, with the CRL immobilized on the NBXCel support.…”

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confidence: 99%

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Biodiesel production using Candida rugosa as biocatalytic lipase immobilized on p‐nitrobenzyl cellulose xanthate (NBXCel)

Rial

Freitas

Cavalheiro

et al. 2021

Biofuels Bioprod Bioref

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This work investigated the best reaction conditions to obtain biodiesel from soybean oil using Candida rugosa lipase (CRL) immobilized on p‐nitrobenzyl cellulose xanthate (NBXCel) support. The alcohols used were methanol and ethanol and the molar ratio used was 1:4 (n/n). The CRL immobilized on NBXCel support showed greater catalytic activity at pH 7.00 and at 45 °C. Thus, 23 experiments were carried out at this pH and at this temperature, varying the reaction time, type of alcohol, and amount of immobilized CRL to achieve a conversion equal to or greater than 96.5% as recommended by EN 14103. The highest conversions of methyl biodiesel were achieved using 4.31%mCRL/mNBXCel and with 3.89%mCRL/mNBXCel. Ethyl biodiesel was achieved at the highest conversion using 5.18% and 4.76%mCRL/mNBXCel. The results were explored with regression analysis to identify the best reaction conditions to obtain a good conversion, and the response surface graphs were plotted to visualize other reaction conditions. We also evaluated the number of reuse cycles of CRL immobilized on NBXCel support, to lower biodiesel production cost, and after 15 cycles of reuse the conversions obtained were above 50%, demonstrating the efficiency of CRL immobilized on the NBXCel support in catalyzing the synthesis of biodiesel. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Biodiesel production using Candida rugosa as biocatalytic lipase immobilized on p‐nitrobenzyl cellulose xanthate (NBXCel)

Rial

Freitas

Cavalheiro

et al. 2021

Biofuels Bioprod Bioref

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“…New nanomaterials (nanostructure) are unique and promising carriers for lipase immobilization. [97][98][99][100][101] These materials exhibit substantial specific surface area, high lipase loading capacity, excellent dispersibility, low mass transfer restriction and other advantages, making them attractive biocatalytic carriers for biodiesel synthesis. 92 In recent years, different types of nanomaterials have received more attention as lipase immobilization carriers for biodiesel synthesis, such as carbon nanotubes, 10 nanoflowers, 102 metal-organic frameworks (MOFs), 92 nanofibers, 103 nanoparticles 104 and graphene oxide, 105…”

Section: New Carrier Materials For Lipase Immobilizationmentioning

confidence: 99%

“…Nanomaterials, mesoporous materials, are the most newly employed carriers for lipase immobilization. New nanomaterials (nanostructure) are unique and promising carriers for lipase immobilization 97–101 . These materials exhibit substantial specific surface area, high lipase loading capacity, excellent dispersibility, low mass transfer restriction and other advantages, making them attractive biocatalytic carriers for biodiesel synthesis 92 .…”

Section: Enzyme Immobilizationmentioning

confidence: 99%

Recent developments of lipase immobilization technology and application of immobilized lipase mixtures for biodiesel production

Abdulmalek

Yan

2022

Biofuels Bioprod Bioref

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Lipases are considered the most widely used industrial biocatalysts, and play a significant role in diverse industrial and biotechnological applications such as in biodiesel. Biodiesel is a renewable and ecologically friendly biofuel, and has received significantly increased attention in recent years. The reaction media of biodiesel synthesis via the transesterification process of fats and oils could be considered cascade reactions. The use of a mixture of immobilized lipases as biocatalysts in biodiesel reactions has received much consideration since it is a ‘greener technique’ and has several advantages over free lipases. The selection of appropriate carrier materials and immobilization techniques fundamentally affects the immobilized lipases’ catalytic performance for biodiesel production. Hence, this review discusses the recent developments for carrier materials and techniques for lipase immobilization as well as the current status of immobilized lipases mixtures and their application for biodiesel synthesis. The initial part of this review presents a brief introduction of lipases and their application for biodiesel preparation. New carrier materials and different basic and recent lipase immobilization techniques are also evaluated. Most significantly, studies carried out on mixtures of immobilized lipase‐catalyzed biodiesel for the last 15 years (2006–2021) are summarized. The economic and environmental evaluation of enzymatic biodiesel production is also presented. In the future, the application of mixed and co‐immobilized lipases may be enlarged to other enzymes and processes. © 2022 Society of Chemical Industry and John Wiley & Sons, Ltd

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Production of high quality biodiesel from sunflower soapstock acid oil as novel feedstock: Catalyzed by immobilized pancreatic lipase

Paluzar

2023

J Americ Oil Chem Soc

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Edible refined oils, which are utilized as raw materials in biodiesel production, have been replaced by by‐products (acid oil, fatty acid, deodorized distillate, and soapstock distillates) obtained from the vegetable oil refining industry, in recent years. This study aims to investigate the production of high quality biodiesel fuel in accordance with the standards (TS EN and ASTM) from sunflower soapstock acid oil. This feedstock was donated by an oil factory in the Thrace region, Turkey. Esterification reaction was performed by immobilized enzyme. For this, immobilization was carried out by covalent binding of pancreatic lipase in glutaraldehyde activated chitosan and optimum immobilization conditions were determined. The activity of the immobilized lipase and the retained activity were found to be 65.69 U/μg and 61.8%, respectively (pH: 7.5, 37°C). The Km (Michealis constant) and Vmax (maximum velocity of an enzymatically catalyzed reaction) values of the immobilized enzyme were found to be 5.1 mmol/L and 48.6 U/min/mg protein, respectively. The immobilized enzyme was employed as a biocatalyst for esterification of sunflower soapstock acid oil. Notably, an impressive yield of 75.6% was attained under the conditions of a 1:5 molar ratio of soapstock acid oil to methanol, with 10 wt% immobilized lipase as the catalyst, and a reaction temperature of 45°C for 36 h. The resulting biodiesel exhibits fuel characteristics that meet the standards outlined in TS EN 14214:2012 + A2 and ASTM D6751‐02.

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Candida rugosa lipase for the biodiesel production from renewable sources

Cited by 29 publications

References 37 publications

Biodiesel production using Candida rugosa as biocatalytic lipase immobilized on p‐nitrobenzyl cellulose xanthate (NBXCel)

Biodiesel production using Candida rugosa as biocatalytic lipase immobilized on p‐nitrobenzyl cellulose xanthate (NBXCel)

Recent developments of lipase immobilization technology and application of immobilized lipase mixtures for biodiesel production

Production of high quality biodiesel from sunflower soapstock acid oil as novel feedstock: Catalyzed by immobilized pancreatic lipase

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