Candida rugosa lipase-catalyzed polyurethane degradation in aqueous medium

Gautam, Rajeeb; Bassi, Amarjeet; Yanful, Ernest K.

doi:10.1007/s10529-007-9354-1

Cited by 61 publications

(45 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Eight of the most active organisms belonged to the Pestalotiopsis genus. The current literature reports some fungi with the ability to degrade PUR (4)(5)(6)8), although these studies have focused primarily on organisms isolated from soil samples. This is the first study that demonstrates PUR degradation by endophytic fungi.…”

Section: Discussionmentioning

confidence: 99%

“…The liquid medium assay followed the method described by Gautam et al (8). PUR-L liquid medium was prepared using the same recipe as for the solid PUR-A medium without agar.…”

Section: Methodsmentioning

confidence: 99%

“…Putative polyurethanases have been isolated and characterized from protein extracts of several organisms, including the bacteria Pseudomonas chlororaphis (25) and Comamonas acidovorans (1,2), as well as the fungus Candida rugosa (8). The active enzymes have been classified as esterases (5,13,16), lipases (26), and proteases and ureases (19), suggesting degradation of the PUR substrate by cleavage of the ester bond.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Biodegradation of Polyester Polyurethane by Endophytic Fungi

Russell

Huang

Anand

et al. 2011

Appl Environ Microbiol

426

190

View full text Add to dashboard Cite

Bioremediation is an important approach to waste reduction that relies on biological processes to break down a variety of pollutants. This is made possible by the vast metabolic diversity of the microbial world. To explore this diversity for the breakdown of plastic, we screened several dozen endophytic fungi for their ability to degrade the synthetic polymer polyester polyurethane (PUR). Several organisms demonstrated the ability to efficiently degrade PUR in both solid and liquid suspensions. Particularly robust activity was observed among several isolates in the genus Pestalotiopsis, although it was not a universal feature of this genus. Two Pestalotiopsis microspora isolates were uniquely able to grow on PUR as the sole carbon source under both aerobic and anaerobic conditions. Molecular characterization of this activity suggests that a serine hydrolase is responsible for degradation of PUR. The broad distribution of activity observed and the unprecedented case of anaerobic growth using PUR as the sole carbon source suggest that endophytes are a promising source of biodiversity from which to screen for metabolic properties useful for bioremediation.Tremendous increases in the manufacture and consumption of plastics over recent decades have led to numerous ecological and economic concerns. The persistence of synthetic polymers introduced into the environment by human industry poses a major threat to natural ecological systems. The low cost and ease of manufacture have increased global plastic demand more than 150-fold, with the production of 1.5 million tons in 1950 and 245 million tons as of 2006 (21). Despite recognition of the persistent pollution problems posed by plastic, global production is still increasing, with the largest increases expected in developing nations. The sheer volume of plastics produced each year presents a problem for waste disposal systems. The scale of this problem and the recalcitrance of some polymers to degradation necessitate investigation into effective methods for biodegradation of plastics. By gaining an understanding of the mechanisms of polymer degradation, a more efficient technique for the biodegradation of plastic waste can be achieved. To accomplish this goal, researchers need greater knowledge of how compounds are metabolized by existing organisms, an investigation of new organisms with bioremediation potential, and the characterization of novel metabolic capabilities.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The liquid medium assay followed the method described by Gautam et al (8). PUR-L liquid medium was prepared using the same recipe as for the solid PUR-A medium without agar.…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Biodegradation of Polyester Polyurethane by Endophytic Fungi

Russell

Huang

Anand

et al. 2011

Appl Environ Microbiol

426

190

View full text Add to dashboard Cite

show abstract

“…Polyurethanase protease activities have been reported for Pseudomonas fluorescens and Pseudomonas chlororaphis (Howard and Blake, 1998;, polyurethanase lipase activity has been detected in Bacillus subtilis strains (Rowe and Howard, 2002). Commerciallyavailable Candida rugosa lipase was successfully used to biodegrade synthetic polyester polyurethane particles in an aqueous medium (Gautam et al, 2007). Polyurethanase esterase activities have been reported for Corynebacterium sp., Comamonas acidovorans TB-35, and P. chlororaphis (Kay et al, 1993;Nakajima-Kambe et al, 1997;.…”

Section: Introductionmentioning

confidence: 99%

Degradation of polyurethane by novel bacterial consortium isolated from soil

et al. 2008

View full text Add to dashboard Cite

The present study describes the isolation of bacteria from soil with the ability to degrade plastic polyurethane (PU). Bacterial strains attached on the polyurethane film, after soil burial for 6 months, were isolated and identified as Bacillus sp. AF8, Pseudomonas sp AF9, Micrococcus sp. 10, Arthrobacter sp. AF11, and Corynebacterium sp. AF12. In plate assay, zones of hydrolysis were visualised around the bacterial colonies on mineral salt agar plates containing polyurethane as a sole carbon source. The results of the Sturm test for degradability showed more CO 2 production in the test than in control. The production of esterase was detected in the presence of polyurethane as a substrate. The Scanning Electron Microscopy and Fourier Transform Infrared Spectroscopy showed certain changes on the surface of PU film and formation of some new intermediate products after polymer breakdown.

show abstract

“…A high molecular weight polymer is primarily oxidized or hydrolyzed by enzymes to functional groups which improve the polymer hydrophilicity. Then, the polymer is depolymerized by microbial enzymes and then absorbed by microbial cells and ruined [10][11][12][13]. Biodegradability of PUs is generally achieved by incorporating labile and hydrolyzable moieties into the polymer backbone.…”

Section: Introductionmentioning

confidence: 99%

Study of Biological Degradation of New Poly(Ether-Urethane-Urea)s Containing Cyclopeptide Moiety and PEG by Bacillus amyloliquefaciens Isolated from Soil

Rafiemanzelat

Jafari

Emtiazi

2015

Appl Biochem Biotechnol

View full text Add to dashboard Cite

The present work for the first time investigates the effect of Bacillus amyloliquefaciens, M3, on a new poly(ether-urethane-urea) (PEUU). PEUU was synthesized via reaction of 4,4'-methylenebis(4-phenylisocyanate) (MDI), L-leucine anhydride cyclopeptide (LACP) as a degradable monomer and polyethylene glycol with molecular weight of 1000 (PEG-1000). Biodegradation of the synthesized PEUU as the only source for carbon and nitrogen for M3 was studied. The co-metabolism biodegradation of the polymer by this organism was also investigated by adding mannitol or nutrient broth to the basic media. Biodegradation of the synthesized polymer was followed by SEM, FT-IR, TGA, and XRD techniques. It was shown that incubation of PEUU with M3 resulted in a 30-44 % reduction in polymer's weight after 1 month. This study indicates that the chemical structure of PEUU significantly changes after exposure to M3 due to hydrolytic and enzymatic degradation of polymer chains. The results of this work supports the idea that this poly(ether-urethane) is used as a sole carbon source by M3 and this bacterium has a good capability for degradation of poly(ether-urethane)s.

show abstract

Candida rugosa lipase-catalyzed polyurethane degradation in aqueous medium

Cited by 61 publications

References 14 publications

Biodegradation of Polyester Polyurethane by Endophytic Fungi

Biodegradation of Polyester Polyurethane by Endophytic Fungi

Degradation of polyurethane by novel bacterial consortium isolated from soil

Study of Biological Degradation of New Poly(Ether-Urethane-Urea)s Containing Cyclopeptide Moiety and PEG by Bacillus amyloliquefaciens Isolated from Soil

Contact Info

Product

Resources

About