Candida parapsilosis CCTCC M203011 and the optimization of fermentation medium for stereoinversion of (S)-1-phenyl-1,2-ethanediol

Mu, Xiao Qing; Xu, Yan; Nie, Yao; Ouyang, Jian; Sun, Zongqiong

doi:10.1016/j.procbio.2004.09.026

Cited by 19 publications

(14 citation statements)

References 16 publications

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“…This work merely focused on the improvement of the composition of YM medium. As reported by Mu et al [19] for the stereoinversion of S-1-phenyl-1,2-ethanediol by C. parapsilosis, many other nutritional ingredients may affect the stereospecificity of cultured yeast's. Therefore, a detailed study on the medium composition design has already been carried out in our laboratory.…”

Section: Discussionmentioning

confidence: 84%

“…Dahl and Madsen [18] indicated that the growth conditions of the yeast, such as pH and oxygen supply greatly influenced the stereoselectivity in the reduction of 3-oxo esters by bakers' yeast. Mu et al [19] demonstrated that the optimization of fermentation medium markedly enhanced the conversion ratio and the cell's stereoselectivity in the stereoinversion of RS-1-phenyl-1,2-ethanediol to S-1-phenyl-1,2-ethanediol by the whole-cell catalysis of Candida parapsilosis.…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of asymmetric bioreduction of ethyl 4-chloro acetoacetate by the design of composition of culture medium and reaction conditions

Houng

Liao

et al. 2007

Process Biochemistry

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Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Enhancement of asymmetric bioreduction of ethyl 4-chloro acetoacetate by the design of composition of culture medium and reaction conditions

Houng

Liao

et al. 2007

Process Biochemistry

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“…Previous research in mono-aqueous phase suggested that potassium phosphate buffer was appropriate for stereoinversion of (R)-PED into (S)-PED catalyzed by C. parapsilosis CCTCC M203011 [7,25] and its high-valent inorganic salts tend to scrabble for water against polymers rather than low-valent inorganic salts for ATPS [26]. Figure 2 shows the effect of concentration of potassium phosphate buffer and the highest e.e.…”

Section: Effects Of Buffer Solutionmentioning

confidence: 99%

Enhancement of substrate concentration in microbial stereoinversion through one-pot oxidation and reduction by aqueous two-phase system

2009

Bioprocess Biosyst Eng

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An extractive biocatalytic method of aqueous two-phase system was employed for stereoinversing (R)-1-phenyl-1,2-ethanediol into (S)-1-phenyl-1,2-ethanediol by Candida parapsilosis CCTCC M203011. It was observed that substrate and product inhibitions in microbial stereoinversion through one-pot oxidation and reduction were removed efficiently by extractive biocatalysis in aqueous two-phase system with PEG 4000/phosphate potassium system, and that the substrate concentration was enhanced from 15 to 30 g/L with product optical purity of 99.02% e.e. and yield of 90% after 60 h. Simultaneously, it was observed that change in cell morphology impedes the further enhancement of substrate concentration in this system but can be reversibly changed after stereoinversion or cultivation in systems without PEG.

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“…Nitrogen which is a main component of protein and nucleic acid plays a key role in cell growth and reproduction [25]. The addition of peptone would provide nitrogen source for the fungal growth and enzyme production, resulted in a higher diosgenin yield.…”

Section: Optimization Of Medium Compositionmentioning

confidence: 99%

“…K ? is a key ion involved in enzyme stereo-inversion reaction [25]. Therefore, supplementation of K 2 HPO 4 was important to enhance diosgenin production.…”

Section: Optimization Of Medium Compositionmentioning

confidence: 99%

Process optimization for the production of diosgenin with Trichoderma reesei

Zhu

Huang

2009

Bioprocess Biosyst Eng

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Based on the response surface methodology, an effective microbial system for diosgenin production from enzymatic pretreated Dioscorea zingiberensis tubers with Trichoderma reesei was studied. The fermentation medium was optimized with central composite design (3(5)) depended on Plackett-Burmann design which identified significant impacts of peptone, K(2)HPO(4) and Tween 80 on diosgenin yield. The effects of different fermentation conditions on diosgenin production were also studied. Four parameters, i.e. incubation period, temperature, initial pH and substrate concentration were optimized using 4(5) central composite design. The highest diosgenin yield of 90.57% was achieved with 2.67% (w/v) of peptone, 0.29% (w/v) of K(2)HPO(4), 0.73% (w/v) of Tween 80 and 9.77% (w/v) of substrate, under the condition of pH 5.8, temperature 30 degrees C. The idealized incubation time was 6.5 days. After optimization, the product yield increased by 33.70% as compared to 67.74 +/- 1.54% of diosgenin yield in not optimized condition. Scale-up fermentation was carried out in a 5.0 l bioreactor, maximum diosgenin yield of 90.17 +/- 3.12% was obtained at an aeration of 0.80 vvm and an agitation rate of 300 rpm. The proposed microbial system is clean and effective for diosgenin production and thus more environmentally acceptable than the traditional acid hydrolysis.

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Candida parapsilosis CCTCC M203011 and the optimization of fermentation medium for stereoinversion of (S)-1-phenyl-1,2-ethanediol

Cited by 19 publications

References 16 publications

Enhancement of asymmetric bioreduction of ethyl 4-chloro acetoacetate by the design of composition of culture medium and reaction conditions

Enhancement of asymmetric bioreduction of ethyl 4-chloro acetoacetate by the design of composition of culture medium and reaction conditions

Enhancement of substrate concentration in microbial stereoinversion through one-pot oxidation and reduction by aqueous two-phase system

Process optimization for the production of diosgenin with Trichoderma reesei

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