Candida nivariensis as a New Emergent Agent of Vulvovaginal Candidiasis: Description of Cases and Review of Published Studies

Aznar-Marín, Pilar; Galán-Sanchez, Fátima; Marín-Casanova, Pilar; García-Martos, Pedro; Rodríguez-Iglesias, Manuel

doi:10.1007/s11046-015-9978-y

Cited by 17 publications

(17 citation statements)

References 25 publications

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“…The strains were isolated from blood, bronchoalveolar lavage fluid (BALF) and urine of three patients over a 3-year study period (Alcoba-Flórez et al 2005). Current knowledge indicates that this species occurs worldwide and the number of published cases reaches 108 in Europe (Spain, the UK, France, Poland) (Borman et al 2008; Gorton et al 2013; López-Soria et al 2013; Parmeland et al 2013; Swoboda-Kopeć et al 2014; Aznar-Marin et al 2016; Angoulvant et al 2016), 31 in Asia (Japan, Indonesia, India, China, Malaysia) (Fujita et al 2007; Wahyuningsih et al 2008; Chowdhary et al 2010; Sharma et al 2013; Li et al 2014; Tay et al 2014; Feng et al 2015; Hou et al 2017), 11 in Australia (Lockhart et al 2009; Pinto et al 2011), four in Latin America (Argentina and Brazil) (Morales-López et al 2016; Figueiredo-Carvalho et al 2016) and one case in Africa (Burkina Faso) (Sanata et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of human pathogens of the clade Nakaseomyces in a culture collection—the first report on Candida bracarensis in Poland

et al. 2018

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Human pathogens belonging to the Nakaseomyces clade include Candida glabrata sensu stricto, Candida nivariensis and Candida bracarensis . Their highly similar phenotypic characteristics often lead to misidentification by conventional laboratory methods. Therefore, limited information on the true epidemiology of the Candida glabrata species complex is available. Due to life-threatening infections caused by these species, it is crucial to supplement this knowledge. The aim of the study was to estimate the prevalence of C. bracarensis and C. nivariensis in a culture collection of C. glabrata complex isolates. The study covered 353 isolates identified by biochemical methods as C. glabrata , collected from paediatric and adult patients hospitalised at four medical centres in Southern Poland. The multiplex PCR was used to identify the strains. Further species confirmation was performed via sequencing and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) analysis. One isolate was recognised as C. bracarensis (0.28%). To our knowledge, it is the first isolate in Poland. C. glabrata sensu stricto species has been confirmed for all the remaining isolates. No C. nivariensis was found. Our study has shown that the prevalence of C. nivariensis and C. bracarensis strains is infrequent. However, it should be emphasised that the incidence of these strains may differ locally and depend on environmental factors and the population.

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Section: Discussionmentioning

confidence: 99%

Prevalence of human pathogens of the clade Nakaseomyces in a culture collection—the first report on Candida bracarensis in Poland

et al. 2018

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“…In one study, four isolates collected from vagina were identified as C. nivariensis by the Bruker MS system (Bruker Daltoniks), with a log score between 1.802 and 2.086 (Aznar-Marin et al, 2015). MALDI-TOF MS has been reported to have the ability to distinguish within the C. glabrata clade, i.e., between C. glabrata, C. nivariensis and C. bracarensis , with scores of <1.700 as C. nivariensis (3/10 strains) and C. bracarensis (1/1 strain; Pinto et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Identification and Antifungal Susceptibility Profiles of Candida nivariensis and Candida bracarensis in a Multi-Center Chinese Collection of Yeasts

et al. 2017

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Candida nivariensis and C. bracarensis are two emerging cryptic species within the C. glabrata complex. Thirteen of these isolates from 10 hospitals in China were studied for their species identification and antifungal susceptibilities. Phenotypic and molecular [rDNA ITS sequencing, D1/D2 sequencing and ITS sequencer-based capillary gel electrophoresis (SCGE)] and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS identification methods were compared for their performance in species identification. Twelve of 13 (92.3%) isolates were identified as C. nivariensis and one as C. bracarensis using ITS sequencing as the reference method. Results obtained by D1/D2 sequencing and ITS SCGE were concordant with ITS sequencing results for all (100%) isolates. SCGE was able to subtype 12 C. nivariensis into four ITS SCGE length types. All isolates failed to be identified by the Vitek MALDI-TOF MS system (bioMérieux), whilst the Bruker MS system (Bruker Daltoniks) correctly identified all C. nivariensis isolates but using a lowered (≥1.700) cut-off score for species assignment; the C. bracarensis isolate was identified but with score <1.700. The Vitek 2 Compact system could not identify 11 C. nivariensis and one C. bracarensis isolate and misidentified the remaining C. nivarensis strain as “C. glabrata.” All isolates were susceptible-dose dependent to fluconazole [minimum inhibitory concentration (MIC) range 0.5–4 μg/mL] and were classed as susceptible to echinocandins (MICs ≤ 0.06 μg/mL). All 13 isolates had low MICs for other azoles (MICs ≤ 0.5 μg/mL), amphotericin B (MICs ≤ 2 μg/mL) and 5-flucytosine (MICs ≤ 0.25 μg/mL). Our results reinforce the need for molecular differentiation of species of C. nivarensis and C. bracarensis. The performance of MALDI-TOF may be improved by adding mass spectral profiles (MSPs) into the current databases. The antifungal susceptibility profile of isolates should be monitored.

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“…Inhibition of quorum sensing molecules with natamycin against C. tropicalis haven been used successfully by researchers (Agustín et al, 2019). However, the number of cases is low possibly due to the impossible phenotypic identification of C. glabrata (Aznar-Marin et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Pathogenesis and Clinical Relevance of Candida Biofilms in Vulvovaginal Candidiasis

et al. 2020

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The ability of Candida spp. to form biofilms is crucial for its pathogenicity, and thus, it should be considered an important virulence factor in vulvovaginal candidiasis (VVC) and recurrent VVC (RVVC). Its ability to generate biofilms is multifactorial and is generally believed to depend on the site of infection, species and strain involved, and the microenvironment in which the infection develops. Therefore, both cell surface proteins, such as Hwp1, Als1, and Als2, and the cell wall-related protein, Sun41, play a critical role in the adhesion and virulence of the biofilm. Immunological and pharmacological approaches have identified the NLRP3 inflammasome as a crucial molecular factor contributing to host immunopathology. In this context, we have earlier shown that Candida albicans associated with hyphae-secreted aspartyl proteinases (specifically SAP4-6) contribute to the immunopathology of the disease. Transcriptome profiling has revealed that non-coding transcripts regulate protein synthesis post-transcriptionally, which is important for the growth of Candida spp. Other studies have employed RNA sequencing to identify differences in the 1,245 Candida genes involved in surface and invasive cellular metabolism regulation. In vitro systems allow the simultaneous processing of a large number of samples, making them an ideal screening technique for estimating various physicochemical parameters, testing the activity of antimicrobial agents, and analyzing genes involved in biofilm formation and regulation ( in situ ) in specific strains. Murine VVC models are used to study C. albicans infection, especially in trials of novel treatments and to understand the cause(s) for resistance to conventional therapeutics. This review on the clinical relevance of Candida biofilms in VVC focuses on important advances in its genomics, transcriptomics, and proteomics. Moreover, recent experiments on the influence of biofilm formation on VVC or RVVC pathogenesis in laboratory animals have been discussed. A clear elucidation of one of the pathogenesis mechanisms employed by Candida biofilms in vulvovaginal candidiasis and its applications in clinical practice represents the most significant contribution of this manuscript.

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Candida nivariensis as a New Emergent Agent of Vulvovaginal Candidiasis: Description of Cases and Review of Published Studies

Cited by 17 publications

References 25 publications

Prevalence of human pathogens of the clade Nakaseomyces in a culture collection—the first report on Candida bracarensis in Poland

Prevalence of human pathogens of the clade Nakaseomyces in a culture collection—the first report on Candida bracarensis in Poland

Identification and Antifungal Susceptibility Profiles of Candida nivariensis and Candida bracarensis in a Multi-Center Chinese Collection of Yeasts

Pathogenesis and Clinical Relevance of Candida Biofilms in Vulvovaginal Candidiasis

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